TY - JOUR AU - Oviya, R. AU - Thiruvudainambi, S. AU - Ramamoorthy, V. AU - Thamizh Vendan, R. AU - Vellaikumar, S. PY - 2022/12/14 Y2 - 2024/03/29 TI - Gas Chromatography Mass Spectrometry (GCMS) analysis of the antagonistic potential of Trichoderma hamatum against Fusarium oxysporum f. sp. cepae causing basal rot disease of onion JF - Journal of Biological Control JA - JBC VL - 36 IS - 1 SE - Research Articles DO - 10.18311/jbc/2022/30754 UR - https://informaticsjournals.com/index.php/jbc/article/view/30754 SP - 17-30 AB - <p><em>Fusarium oxysporum</em> f. sp. <em>cepae</em> causing basal rot disease of onion is a destructive phytopathogen resulting in 30-50% yield loss and remains as a major constraint in onion productivity. The management of disease through application of fungicide is not feasible and economically viable. Hence, the present study is focused on investigation of effective <em>Trichoderma</em> sp. and identifying the effective volatile organic compounds produced by it against the basal rot pathogen in onion. A total of ten <em>Trichoderma</em> spp. were isolated from rhizospheric soil of healthy onion plants and tested against virulent <em>Fusarium oxysporum</em> f. sp. <em>cepae</em> isolate FCIM1. The <em>Trichoderma</em> isolate (TIM2) showed 77.40% inhibition on mycelial growth of pathogen followed by the isolate (TIV1) with 70.36% inhibition. The molecular identification of effective <em>Trichoderma</em> isolate through the analysis of the rDNA of Internal Transcribed Spacers (ITS) region revealed isolate TIM2 as <em>Trichoderma hamatum</em>. The GC-MS analysis of <em>Trichoderma hamatum</em> unravelled the important volatile organic compounds like Methyl stearate, n-Hexadecanoic, Eicosane, 9-cyclohexy, Heptadecane, Dodecane, 2-cyclohexyl, to 2H-Pyran-2-one, 6-pentyl, 5-Hydroxymethylfurfural, Tetrapentacontane, 1-Dodecanol, 2-Propenoic acid, pentadecyl ester, Benzene, (2-methylbutyl) and 1,2-Dimethyltryptamine with peak area and retention time. These bioactive compounds exert a strong antifungal activity against <em>Fusarium oxysporum</em> f. sp. <em>cepae</em>. The scanning electron micrographs of <em>Fusarium</em> paired with effective <em>Trichoderma</em> (TIM2) showed the swollen hyphae with cell wall damage which is clear evident of antagonistic interaction of volatile compounds produced by <em>Trichoderma hamatum</em>.</p> ER -