Short-Term in Situ Action of Melatonin on Ion Transport in Mice Kept at Restraint Stress


  • Department of Zoology, University of Kerala, Kariavattom, Thiruvananthapuram 695 581, Kerala
  • Department of Zoology, University of Kerala, Kariavattom, Thiruvananthapuram 695 581, Kerala



ATPase, Melatonin, Mice, Stress.


Melatonin, a pleiotropic hormone, is involved in many physiological functions including combating oxidative stress. However, its role in ion transport during stress response is not yet understood. The dose-dependent effect of in situ melatonin was examined in Swiss albino mice. Perfusion of melatonin at 10-7 M for 20 minutes produced a significant decrease in Na+, K+-ATPase activity in the kidney, liver, stomach and intestinal tissues. A dose-responsive decrease in cytosolic and mitochondrial H+ ATPase activity was found in these tissues after melatonin perfusion. Likewise, the cytosolic and mitochondrial Ca2+ ATPase activities decreased in the kidney, liver, stomach and intestine. The mitochondrial Mg2+ ATPase activity decreased in the tested tissues in a dose-responsive manner. Subjecting mice to restraint stress for seven days increased the Na+, K+-ATPase, H+ ATPase, Ca2+ ATPase and Mg2+ ATPase activities to significant levels in kidney, liver, stomach and intestinal tissues. On the contrary, in-situ perfusion of melatonin to stressed mice at 10-9 M caused decrease in the stress-induced hyperactivity of these transmembrane ion transporters. These results provide evidence for a role of melatonin in ion transporter activity and point to a protective role of melatonin in ion transport during stress response in mice.


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How to Cite

Sajeeb Khan, A., & Subhash Peter, M. C. (2015). Short-Term <I>in Situ Action</I> of Melatonin on Ion Transport in Mice Kept at Restraint Stress. Journal of Endocrinology and Reproduction, 19(1), 20–33.



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