Endocrine, Metabolic and Ovarian Features of Human PCO Repeat in Sprague Dawley Rats- An Experimental Study


  • St. Thomas College, Research Department of Zoology, Kozhencherry, Kerala, 689642, India
  • Pushpagiri Research Centre, Pushpagiri Institute of Medical Sciences, Molecular Biology and Phytoceuticals Research Laboratory, Thiruvalla, Kerala, 689101, India


Polycystic Ovary Syndrome (PCOS) affects 9-18 % of women in the reproductive age group, and is manifested as hyperandrogenism and infertility. However, the etiology of PCOS is still not clear, so knowledge from experimental animal models may perhaps enhance the knowledge regarding the mechanisms of establishment and advancement of PCOS. The present study was undertaken to validate the role of high fructose intake and the consequent alterations in the hormone levels as the causative factors of polycystic ovaries in females. Healthy, young colony-bred female albino rats, Sprague Dawley breed, weighing 80-85 g, were divided into three groups. Rats in group I served as placebo control. Group II rats received fructose, 10 g/10 mL/kg body weight (bw) per day through oral gavage. Group III rats received ip injection of insulin at 0.5 IU/kg bw/day. The treatment lasted 90 days. A significant (p<0.05) increase was detected in FSH, LH, insulin, testosterone and estradiol levels in fructose- and insulin-treated animals compared to the control. The levels of blood glucose, protein, cholesterol and triglyceride were significantly (p<0.05) increased in the treated groups. Cytoplasmic vacuolation, altered hepatic sinusoids, hepatic necrosis, etc., were observed in liver sections of treated rats. The histoarchitecture of ovary of treated animals presented fluid-filled cysts without granulosa cells and follicles. In the treated animals, histolpathological examination of fallopian tubal segments revealed infiltration of inflammatory cells with harshly crowded epithelial cells. Thus, fructose and insulin treatments reiterate certain endocrine, metabolic and ovarian characteristics of human polycystic ovary in therat.


Estradiol, Histoarchitecture, Polycystic Ovarian Syndrome, Testosterone

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