Anti-elastase, Antioxidant, Total phenolic and Total Flavonoid Content of Macassar Kernels (Rhus javanica L) from Pananjung Pangandaran Nature Tourism Park- Indonesia

Jump To References Section

Authors

  • Pharmacy Faculty, University of Indonesia, Depok ,ID
  • Department of Pharmacognosy, Phytochemistry, and Natural Materials, University of Indonesia, Depok ,ID
  • Department of Pharmaceutical Technology, University of Indonesia, Depok ,ID

DOI:

https://doi.org/10.18311/jnr/2020/24240

Keywords:

Antielastase, Antioxidant, Rhus javanica L., Total Flavonoid Content, Total Phenolic Content
pharmacognosy and phytochemistry

Abstract

Background: Phytochemicals are present as important substances in natural resources and therefore, have aided in invention of the anti-oxidant, and anti-elastase properties of polyphenol compounds present in Indonesian herbs such as Macassar Kernels (Rhus javanica L). Objective: This research aimed to investigate anti-elastase, and antioxidant properties. Methods: Ethanolic extract of Rhus Leaves (RL), Rhus Stem (RS), Rhus Greenish Fruit (RGF), and Rhus Blackish-grey Fruit (RBF) were prepared individually by solvent extraction method. Anti-elastase activity was carried out with elastase from porcine pancreas. For quantitative phytochemical screening, DPPH radical scavenging assay, Ferric Reducing Antioxidant Power Assay (FRAP), Total Phenolic Content (TPC), Total Flavonoid Content (TFC) of Rhus javanica were estimated. Result: RS showed highest anti-elastase activity (45.30%±0.087), compared with RL (12.30%±0.004), and there were no activities in RGF and RBF. In DPPH assay, RS had lowest activity (IC50 561.05 μg/ml), compared with RBF (IC50 239.28 μg /ml), RGF (IC50 189.3I μg/ml), and RL (IC50 157.81 μg/ml). RS also has lowest FRAP activity (% inhibition = 26.60%±0.002), and TPC value (28.50±0.03 mgGAE/g dry weight). Conclusion: Test extracts showed anti-aging properties in different mechanisms. RS possessed the highest anti-elastase activity but had the weakest antioxidant activity.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Downloads

Published

2020-04-09

How to Cite

Bunga Anggraini, N., Elya, B., & Iskandarsyah, . (2020). Anti-elastase, Antioxidant, Total phenolic and Total Flavonoid Content of Macassar Kernels (<i>Rhus javanica</i> L) from Pananjung Pangandaran Nature Tourism Park- Indonesia. Journal of Natural Remedies, 20(1), 61–67. https://doi.org/10.18311/jnr/2020/24240

Issue

Section

Short Communication
Received 2019-09-19
Accepted 2020-01-23
Published 2020-04-09

 

References

Rinnerthaler M, Bischof J, Streubel MK, Trost A, Richter K. Oxidative stress in human aging skin. Biomolecules. 2015; 5:545–89. https://doi.org/10.3390/biom5020545. PMid:25906193. PMCid:PMC4496685

Pandel R, Poljšak B, Godic A, Dahmane R. Skin photoaging and the role of antioxidants in its prevention. ISRN Dermatology. 2013:1–11. https://doi.org/10.1155/2013/930164. PMid:24159392. PMCid:PMC3789494

Kammeyer A, Luiten R. Oxidation events and skin aging. Ageing Research Reviews. 2015; 21:16–29. https://doi.org/10.1016/j.arr.2015.01.001. PMid:25653189

Weihermann AC, Lorencini M, Brohem CA, Carvalho CM. Elastin structure and its involvement in skin photoageing. International Journal of Cosmetic Science. 2016; 39(3):241– 7. https://doi.org/10.1111/ics.12372. PMid:27731897

You Y-O, Choi N-Y, Kang S-Y, Kim K-J. Antibacterial activity of rhus javanica against methicillin-resistant staphylococcus aureus. Evidence-Based Complementary and Alternative Medicine. 2013:1–8. https://doi.org/10.1155/2013/549207. PMid:24223060. PMCid:PMC3816054

Moon J, Yim E, Song G, Lee NH, Hyun C. Screening of elastase and tyrosinase inhibitory activity from Jeju Island plants. EurAsian Journal of BioSciences. 2010; 4(1):41–53. https://doi.org/10.5053/ejobios.2010.4.0.6

Ha SJ, Lee J, Kim H, Song K-M, Lee NH, Kim YE. Preventive effect of Rhus javanica extract on UVB-induced skin inflammation and photoaging. Journal of Functional Foods. 2016; 27:589–99. https://doi.org/10.1016/j.jff.2016.10.011

Cho J, Lee K, Park S, Jeong WC, Moon J, Ham K. Isolation and identification of α-glucosidase inhibitors from the stem bark of the nutgall tree (Rhus javanica Linné). Journal of the Korean Society for Applied Biological Chemistry. 2013; 56(5):547–52. https://doi.org/10.1007/ s13765-013-3140-7

Bobo-Garcí­a G, Davidov-Pardo G, Arroqui C, Ví­rseda P, Marí­n-Arroyo MR, Navarro M . Intra-laboratory validation of microplate methods for total phenolic content and antioxidant activity on polyphenolic extracts, and comparison with conventional spectrophotometric methods. Journal of the Science of Food and Agriculture. 2014; 95(1):204–9. https://doi.org/10.1002/jsfa.6706. PMid:24756821

Zhang C, Ma Y, Zhao Y, Hong Y, Cai S, Pang M. Phenolic composition, antioxidant and pancreatic lipase inhibitory activities of Chinese sumac (Rhus chinensis Mill.) fruits extracted by different solvents and interaction between myricetin-3-O-rhamnoside and quercetin-3-Orhamnoside. International Journal of Food Science and Technology. 2017; 53(4):1045–53. https://doi.org/10.1111/ ijfs.13680

Fernandes RPP, Trindade MA, Tonin FG, Lima CG, Pugine SMP, Munekata PES, et al. Evaluation of antioxidant capacity of 13 plant extracts by three different methods: Cluster analyses applied for selection of the natural extracts with higher antioxidant capacity to replace synthetic antioxidant in lamb burgers. Journal of Food Science and Technology. 2015; 53(1):451–60. https://doi.org/10.1007/s13197-0151994-x. PMid:26787964. PMCid:PMC4711430

Tomasina F, Carabio C, Celano L, Thomson L. Analysis of two methods to evaluate antioxidants. Biochemistry and Molecular Biology Education. 2012; 40(4):266–70. https:// doi.org/10.1002/bmb.20617. PMid:22807430

Wittenauer J, Mäckle S, SuíŸmann D, Schweiggert-Weisz U, Carle R. Inhibitory effects of polyphenols from grape pomace extract on collagenase and elastase activity. Fitoterapia. 2015; 101:179–87. https://doi.org/10.1016/j.fitote.2015.01.005. PMid:25598188

Popoola OK, Marnewick JL, Rautenbach F, Ameer F, Iwuoha EI, Hussein AA. Inhibition of oxidative stress and skin aging-related enzymes by prenylated chalcones and other flavonoids from Helichrysum teretifolium. Molecules. 2015; 20(4):7143–55. https://doi.org/10.3390/ molecules20047143. PMid:25903365. PMCid:PMC6272301

Shannon E, Jaiswal A, Abu-Ghannam N. Polyphenolic content and antioxidant capacity of white, green, black, and herbal teas: A kinetic study. Food Research. 2017; 2(1):1– 11. https://doi.org/10.26656/fr.2017.2(1).117

Farasat M, Nejad RA, Nabavi SM, Namjooyan F. Antioxidant activity, total phenolics and flavonoid contents of some edible green seaweeds from Northern Coasts of the Persian Gulf. Iranian Journal of Pharmaceutical Research. 2014; 13(1):163–70.

Moharram HA, Youssef MM. Methods for determining the antioxidant activity: A Review = داضملا طاشنلا ريدقت قرط يعجرم ضارعتسا : ةدسكألل . Alexandria Journal of Food Science and Technology. 2014; 11(1):31241. https://doi.org/10.12816/0025348

Маkаrеnkо О, Levitsky Ð. Biochemical mechanisms of therapeutic and prophylactic effects of bioflavonoids. Journal of Pharmacy and Pharmacology. 2016; 4(8). https:// doi.org/10.17265/2328-2150/2016.08.013