Journal Logo   Journal of Natural Remedies

Authenticating the Anti-cancer Properties of Couroupita guianensis in Western Ghats using HL60 Humanleukemia Cell Line


Affiliations

  • Bannari Amman Institute of Technology, Department of Biotechnology, Sathyamangalam, Tamil Nadu, 638401, India

Abstract

The target of this work is to evaluate the in–vitro anticancer activity and cellular toxicity of Couroupita guianensis (CG) towards the Human promyelocytic leukemia HL60 cells contrasted with the normal cell lines. The anti-cancer properties of Couroupita guianensis extracted with Chloroform and DMSO (Dimethyl sulfoxide) were analysed and studied throughout this paper. HL60 cell lines were permitted to develop in DMEM (Dulbecco's Modified Eagle's medium) and incubated with differed concentration of DMSO CG extract. MTT assay drew out the best approach to decide the cell feasibility and assessment was done with the optical absorbance at 570nm and 620nm as reference. The floral extract inhibited 50% growth (IC50) of HL60 cell lines at 10 mg/ml of extract concentration. Inhibitory adequacy of CG showed the cell – plausibility in time and dosage relied route with predictable morphological changes. Flow cytometer assessed the apoptosis insisting the cell toxicity esteem for MTT at IC50 with 70 % cell suitability. Morphological investigation likewise plainly expresses that no apoptosis has been found in charge and comparably in CG treated when contrasted with destructive HL60 cell – line. Assessment of cell toxicity impact of CG demonstrated it can be an inert source of great transformation in human Acute promyelocytic leukemia indicating (revealing) that chemotherapeutic agent.

Keywords

Cellular Toxicity, Growth Inhibition, Leukemia DMSO

Full Text:

References

Moorthi V, Jasmine SF. Couroupita guianensis: the reservoir of medicinal compounds of human welfare. Asian J Pharm Clin Res. 2017; 10:50–2. https://doi. org/10.22159/ajpcr.2017.v10i3.16174

Kekuda TP. Antimicrobial, radical scavenging, and insecticidal activity of leaf and flower extracts of Couroupita guianensis Aubl. Int. J. Green Pharm. 2017; 11:55–8.

Gousia SK, Kumar KA, Kumar TV, Latha JNL. Biological activities and medicinal properties of Couroupita guianensis. Int J Pharm Pharm Sci. 2013; 3:140–3.

Bergman J, Lindström JO, Tilstam ULF. The structure and properties of some indolic constituents in Couroupita guianensis Aubl. Tetrahedron. 1985; 41:2879–81. https://doi.org/10.1016/S0040-4020(01) 96609-8

Ramachandran RP, Madhivanan S, Sundararajan R, Lin CWY, Sankaranarayanan K. An in vitro study of electroporation of leukemia and cervical cancer cells. Electroporation-Based Therapies for Cancer: From Basics to Clinical Applications. 2014; 12:161–83. https://doi.org/10.1533/9781908818294.161

Collins SJ. The HL-60 promyelocytic leukemia cell line: Proliferation, differentiation, and cellular oncogene expression. Blood. 1987; 70(5):1233–44. https://doi.org/10.1182/blood.V70.5.1233.1233. PMid:3311197

Panchal HN, Desai SD, Soni MK, Mishra P, Meshram DB. In-vitro antiurolithiatic and antioxidant activity of Couroupita guianensis aubl leaves. Pelagia Research Library Der Pharmacia Sinica. 2012; 35:3455–77.

Uppala PK, Kumar KA, Dadi JVR, Gorla US. Evaluation of antimicrobial activity of the aqueous and chloroform extracts of leaves of Couroupita guianensis by well diffusion method. Eur J Pharm Med Res. 2016; 3(11).

Sumathi S, Anuradha R. Couroupita guianensis Aubl: An updated review of its phytochemistry and pharmacology. Asian J Pharm Pharmacol. 2017; 3:1–8.

Premanathan M, Radhakrishnan S, Kulangiappar K, Singaravelu G, Thirumalaiarasu V, Sivakumar T, et al. Antioxidant and anticancer activities of isatin (1H-indole-2, 3-dione), isolated from the flowers of Couroupita guianensis Aubl. Indian J Med Res. 2012; 136:822–4.

Bharathi TR. International Journal of Phytopharmacy Research Article. The Indian journal of medical research, 2015; 12:22-26.

Keerthana V, Devakumar J, Sudha SS. Qualitative analysis of phytochemicals, antibacterial activity, and mycelial growth inhibition of three different plants. Drug Invent. Today. 2018; 10.

Singh R, Kumari N, Gangwar M, Nath G. Qualitative characterization of phytochemicals and in vitro antimicrobial evaluation of leaf extract of Couroupita guianensis Aubl. - A threatened medicinal tree. Int. J. Pharm. Pharm. Sci. 2015; 7:212–15.

Al-Dhabi NA, Balachandran C, Raj MK, Duraipandiyan V, Muthukumar C, Ignacimuthu S, Rajput VS. Antimicrobial, antimycobacterial and antibiofilm properties of Couroupita guianensis Aubl. fruit extract. BMC Complement Altern Med. 2012; 12:242. https://doi.org/10.1186/1472-6882-12-242. PMid:23206492. PMCid:PMC3537531

Vaijanathappa J. Phytochemical screening, antioxidant and antiproliferative activities of successive extracts of Couroupita guianensis Aubl. plant. Indian J Nat Prod Resour. 2018; 9:22–7.

Kavitha R, Kamalakannan P, Deepa T, Elamathi R, Sridhar S, Kumar JS. In vitro antimicrobial activity and phytochemical analysis of Indian medicinal plant Couroupita guianensis Aubl. J Chem Pharm Res. 2011; 3:115–21.

Bahuguna A, Khan I, Bajpai VK, Kang SC. MTT assay to evaluate the cytotoxic potential of a drug. Bangladesh J. Pharmacol. 2017; 12:234–84. https:// doi.org/10.3329/bjp.v12i2.30892


Refbacks

  • There are currently no refbacks.