Authenticating the Anti-cancer Properties of Couroupita guianensis in Western Ghats using HL60 Humanleukemia Cell Line


Affiliations

  • Bannari Amman Institute of Technology, Department of Biotechnology, Sathyamangalam, Tamil Nadu, 638401, India

Abstract

The target of this work is to evaluate the in–vitro anticancer activity and cellular toxicity of Couroupita guianensis (CG) towards the Human promyelocytic leukemia HL60 cells contrasted with the normal cell lines. The anti-cancer properties of Couroupita guianensis extracted with Chloroform and DMSO (Dimethyl sulfoxide) were analysed and studied throughout this paper. HL60 cell lines were permitted to develop in DMEM (Dulbecco's Modified Eagle's medium) and incubated with differed concentration of DMSO CG extract. MTT assay drew out the best approach to decide the cell feasibility and assessment was done with the optical absorbance at 570nm and 620nm as reference. The floral extract inhibited 50% growth (IC50) of HL60 cell lines at 10 mg/ml of extract concentration. Inhibitory adequacy of CG showed the cell – plausibility in time and dosage relied route with predictable morphological changes. Flow cytometer assessed the apoptosis insisting the cell toxicity esteem for MTT at IC50 with 70 % cell suitability. Morphological investigation likewise plainly expresses that no apoptosis has been found in charge and comparably in CG treated when contrasted with destructive HL60 cell – line. Assessment of cell toxicity impact of CG demonstrated it can be an inert source of great transformation in human Acute promyelocytic leukemia indicating (revealing) that chemotherapeutic agent.

Keywords

Cellular Toxicity, Growth Inhibition, Leukemia DMSO

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