Development and Validation of Analytical Method for Estimation of Calcium in Herbomineral Formulation by Atomic Absorption Spectrophotometry
Keywords:AAS, Bhasma, Complexometric Titration, Maxcal-C, Wet Digestion
Calcium determination is normally done by conventional titrimetric methods (complexometric titration). In this study, a simple, precise, accurate, rapid and sensitive method has been developed for calcium (Ca) determination in Maxcal-C Tablet (herbo-mineral formulation) and its raw materials by Atomic Absorption Spectrophotometer (AAS). For complete digestion of the sample, closed vessel wet digestion technique was used with 69% HNO3 and 30% H2O2 (7:1) as digestive solvents. Analysis was performed on AAS (AA-6300) at λmax 422.7 nm. Calcium nitrate Ca(NO3)2 was used as a standard for Calcium and the linearity of the standard was excellent over a concentration range of 0.5 to 20 μgmL-1. The regression coefficient (r2) was 0.999. For intraday and interday precision % RSD was obtained between 0.48%-1.95% and 3.48%-5.01%, respectively which complied with the standard range for AAS. Recovery was achieved at 93.24%, 99.73%, 101.75% for 3 levels of spikes (80%, 100%, 120%) respectively, which complied with the standard range 75- 125% for AAS. However, the AAS method produced accurate and reproducible results and also overcame the colour and other ions interference, which are common problems with the titrimetric method. The results of both techniques (the AAS method and the titrimetric method) were compared, which showed good estimation by AAS.
Garg M, Das S, Jaspreet S, Sukender K. AS estimation of Heavy Metals and Trace elements in Indian Herbal Cosmetics Preparation. Research Journal of Chemical Science. 2012; 2(3):46-51.
Rasmussen R, Hedegaard R. Development and validation of an SPE HG-AAS method for determination of inorganic arsenic in sample of marine origine. Anal Bioanal Chem. 2012; 2825-2834. https://doi.org/10.1007/s00216-012-6006-7 DOI: https://doi.org/10.1007/s00216-012-6006-7
Ayivor, Okine L, Nyarko B, Debrah S. The application of Westcott Formalism k0 NAA method to estimate short and medium lived elements in some Ghanaian herbal medicines complemented by AAS. Radiation Physics and Chemistry. 2012; 81:403-409. https://doi.org/10.1016/j. radphyschem.2011.12.034 DOI: https://doi.org/10.1016/j.radphyschem.2011.12.034
Carneiro D, Pedrini G, Teraza M, Henrique F. Determination of Na, K, Ca, Mg in biodiesel samples by flame AAS using microemulsion as sample preparation. Microchemical. 2010; 96:180-185. https://doi.org/10.1016/j. microc.2010.03.005 DOI: https://doi.org/10.1016/j.microc.2010.03.005
Njoku P, Ohia C. Spectrophotometric estimation studies of Mineral nutrient in Three Cocoyam Cultivators. Pakistan Journal of Nutrition. 2007; 6:616-619.https://doi. org/10.3923/pjn.2007.616.619 DOI: https://doi.org/10.3923/pjn.2007.616.619
Hseu Z. Evaluating heavy metal contents in nine composts using four digestion methods. Bioresource Technology. 2004; 95:53-59. https://doi.org/10.1016/j.biortech.2004.02.008 DOI: https://doi.org/10.1016/j.biortech.2004.02.008
Soylak M, Tuzen M, Narin I, Sari H. Comparison of Microwave, Dry and Wet Digestion Procedures for the Determination of Trace Metal Contents in Spice Samples Produced in Turkey. Journal of Food and Drug Analysis. 2004; 12(3):254-258. https://doi.org/10.38212/2224-6614.2634 DOI: https://doi.org/10.38212/2224-6614.2634
Bastos L, Soarse M, Correia L. Validation of an Electrothemal Atomic Absorption Spectrometry Method for the Determination of Aluminum, Copper and Lead in grapes. Toxicology Department, Faculty of Pharmacy, University of Porto, Portugal.
Alarcon M, Lopez M, Lopez M, Segado A. Magnesium and Calcium content in food drom SE Spain: influencing factors and estimation pf daily dietary products. The Science of the Total Environment. 2003; 312:47-58. https:// doi.org/10.1016/S0048-9697(03)00199-2 DOI: https://doi.org/10.1016/S0048-9697(03)00199-2
Mocak J, Borosova D, Miskovic P. Validation and Quality Assurance of Arsenic determination in Urine by GFAAS after toluene extraction. Polish Journal of Environmental studies. 2002; 11:617-623.
Barbas C, Marin A, Gonzalvez A. Development and validation of extraction methods for determination of zinc and arsenic speciation in soils using focused ultrasound application to heavy metals study in mud and soils. Analytica Chimica Acta. 2001; 442:305-318. https://doi.org/10.1016/ S0003-2670(01)01169-2 DOI: https://doi.org/10.1016/S0003-2670(01)01169-2
Soylak M, Dogan M, Narin I, Elci L. Determination of trace metal ions by AAS in natural water samples after preconcentration of pyrocatechol violet complexes on an activated carbon column. Talanta. 2000; 52:1041-1046. https://doi. org/10.1016/S0039-9140(00)00468-9 DOI: https://doi.org/10.1016/S0039-9140(00)00468-9
Pybus J, Feldman F, Bowers G. Measurement of total Calcium in serum by Atomic Absorption Spectrometry with use of a strontium Internal Reference. Journal of clinical chemistry. 1970; 6:338-1007. https://doi.org/10.1093/ clinchem/16.12.998 DOI: https://doi.org/10.1093/clinchem/16.12.998
Thin C, Thomson P. Estimation of Calcium and Magnesium in serum and urine by Atomic Absorption Spectrometry. Journal of clinical pathology.1967; 20:280-282. https://doi. org/10.1136/jcp.20.3.280 DOI: https://doi.org/10.1136/jcp.20.3.280
Anthony P. Atomic absorption spectrometric determination of calcium and other metallic elements in some animal protein source. Talanta. 2000; 52:749-754. https://doi. org/10.1016/S0039-9140(00)00368-4 16. Krolak E, Zdanowski B. Phosphrous and calcium in the mussels Sinanodonta woodiana and dreissena polymorpha in konin lakes. Archieves of Polish Fisheries. 2007; 15:287-294. DOI: https://doi.org/10.1016/S0039-9140(00)00368-4
Pinheiro J, Diniz A, Vasconcellos M, Soido C. An improvement of calcium determination technique in shell of Molluscs. Brazilian archieves of biology and technology. 2009; 52:93-98. https://doi.org/10.1590/S1516- 89132009000100012 DOI: https://doi.org/10.1590/S1516-89132009000100012
Indian Pharmacopoeia, Indian pharmacopoeial commission. Ghaziabad, 2010; 2:962.
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