Lysozyrne-DPPC Interactions in the Model Membrane, DPPC-H2O

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Authors

  • Solid State Physics Division, Bhabha Atomic Research Centre, Trombay, Bombay 400085 ,IN
  • Biochemistry Division, Bhabha Atomic Research Centre, Trombay, Bombay 400085 ,IN
  • Applied Chemistry Divison, Bhabha Atomic Research Centre, Trombay, Bombay 400085 ,IN
  • Solid State Physics Division, Bhabha Atomic Research Centre, Trombay, Bombay 400085 ,IN
  • Solid State Physics Division, Bhabha Atomic Research Centre, Trombay, Bombay 400085 ,IN
  • Sophisticated Instruments Facility, Indian Institute of Science, Bangalore 560012 ,IN
  • Sophisticated Instruments Facility, Indian Institute of Science, Bangalore 560012 ,IN
  • Sophisticated Instruments Facility, Indian Institute of Science, Bangalore 560012 ,IN
  • Sophisticated Instruments Facility, Indian Institute of Science, Bangalore 560012 ,IN

DOI:

https://doi.org/10.18311/jsst/1992/2303

Keywords:

DSC, 1H NMR, Fluorescence, Model Membrane, Lysozyme, Protein-Lipid Interactions.

Abstract

The effect of the protein, lysozyme, on the hydrocarbon chain melting phase trasition of the model membrane system, DPPC-H2O/D2O, as been investigated by DSC, 1H NMR and fluorescence spectroscopy. It is found that the protein binds to the lipid headgroups and that the effective lipid headgroup-headgroup interaction is increased due to lipid-protein interaction. The tryptophan residue(s) of the proteinis implicated in thisassociation. The interaction isstronger when the membrane is in the form of vesicles rather than that of lamellar stacks. The lipid headgroup-lysozyrne binding results in reduced mobility of the acyl chains as well as the headgroup of the lipid molecule.