Voltammetric Approach of Arsenic (Total) Determination in Blood using Sctrace Gold Electrode


  • Kamakshi Mehta Department of Anatomy, All India Institute of Medical Sciences, New Delhi – 110029
  • Rohit Kanojia Department of Chemistry, University of Delhi, New Delhi – 110007
  • A. K. Jaiswal Department of Forensic Medicine and Toxicology, All India Institute of Medical Sciences, New Delhi – 110029
  • Tabin Millo Department of Forensic Medicine and Toxicology, All India Institute of Medical Sciences, New Delhi – 110029




Arsenic, Blood, scTrace Gold Electrode, Voltammetry


Normal levels of Arsenic (As) in the blood of unexposed individuals arereported to be less than 1 μg/L. The quantitative determination of arsenic traces and its compounds is significant to assess its deleterious effects on human health. The use of conventional methods (AAS and ICP–MS) for trace metal detection extend several issues such as high cost of equipment, need of highly trained engineers and extensive sample preparations. An alternativevoltammetric method has been developed for arsenic determination in blood using scTrace Gold elcetrode. The scTrace Gold sensor holds all three electrodes together required for a voltammetric determination. The working electrode is a gold microwire whereas reference and auxiliary electrodes are screen printed electrodes. Human blood was processed by closed microwave digestion using nitric acid and hydrogen peroxide. Arsenic determination was carried out by standard addition method using primary solution being swept at a rate of 0.992 V/s and pulse amplitude of 0.020 V. Cleaning was done at – 1.0V for 60 seconds and potential was scanned from 0.4V to -1.0V on RDE/SSE at 2400 rpm speed. With this method, the total arsenic i.e., As(III) + As(V) in the sample can be determined. As(V) species being electrochemically inactive are reduced in-situ by nascent hydrogen to As (III). Along with the As(III) present in the sample, it is further reduced electrochemically to As(0) and deposited on the gold working electrode in the same step. During the subsequent stripping step the deposited As(0) is reoxidized to As(III) giving the analytical signal. Arsenic was deposited on the electrode at -0.250 V for 5 seconds. The deposited metal was swept by scanning the potential from -0.300V to 0.40 V using square wave mode. The stripping current was correlated with the concentration of the metal present in the sample. The detection limit of arsenic was found to be 0.9 μg/L and the calibration was linear up to a concentration of 20 μg/L.


Tchounwou PB, Yedjou CG, Patlolla AK, Sutton DJ. Heavy metal toxicity and the environment. Molecular, Clinical and Environmental Toxicology. Basel: Springer; 2012. p. 133–64. PMid:22945569 PMCid:PMC4144270. https:// doi.org/10.1007/978-3-7643-8340-4_6

World Health Organization (WHO). Health risks of heavy metals from long range trans boundary air pollution. Jt. WHO l Conv. Task Force Heal. Asp. Air Pollution. 2007. p. 2–144.

World Health Organization (WHO).| Ten chemicals of major public health concern. 2010.

Pereira JA, Das P, Chaklader M, Chatterjee S, Basak P, Chaudhuri S et al. Effects of inorganic arsenic on bone marrow hematopoietic cells: An emphasis on apoptosis and Sca-1/c-Kit positive population. Journal of Stem Cells. 2011; 5(3):117–27.

Hughes MF, Beck BD, Chen Y, Lewis AS, Thomas DJ. Arsenic exposure and toxicology: A historical perspective. Toxicological Sciences. 2011 1; 123(2):305– 32. PMid:21750349 PMCid:PMC3179678. https://doi.org/10.1093/toxsci/kfr184

Lestarini DT, Ivandini TA. Electrochemical detection of As3+ and As5+ by anodic stripping voltammetry at a gold electrode. IOP Conference Series: Materials Science and Engineering 2019; 496(1): 012030. IOP Publishing. https://doi.org/10.1088/1757-899X/496/1/012030

Dahal BM, Fuerhacker M, Mentler A, Karki KB, Shrestha RR, Blum WE. Arsenic contamination of soils and agricultural plants through irrigation water in Nepal. Environmental Pollution. 2008 Sep 1;155(1):157–63. PMid:18068879. https://doi.org/10.1016/j.envpol.2007.1 0.024

World Health Organization. Arsenic in drinking-water: Background document for development of WHO guidelines for drinking-water quality. World Health Organization; 2003.

Singh N, Kumar D, Sahu AP. Arsenic in the environment: Effects on human health and possible prevention. Journal of Environmental Biology. 2007; 28(2):359.

Yang HC, Rosen BP. New mechanisms of bacterial arsenic resistance. Biomedical Journal. 2016; 39(1):5– 13. PMid:27105594 PMCid:PMC6138428. https://doi.org/10.1016/j.bj.2015.08.003

Jadoon S, Malik A. DNA damage by heavy metals in animals and human beings: An overview. Biochem Pharmacol. 2017; 6(3):1–8. https://doi.org/10.4172/2167 -0501.1000235

Ratnaike RN. Acute and chronic arsenic toxicity. Postgraduate Medical Journal. 2003; 79(933):391–6. PMid:12897217 PMCid:PMC1742758. http://dx.doi.org /10.1136/pmj.79.933.391

Tan TH, Chen YH, Hsu CC, Lai CC. Intraabdominal Radiopacities after Arsenic Intoxication. Journal of Emergency Medicine. 2015; 49(5):e159–60. PMid:26234715. https://doi.org/10.1016/j.jemermed.20 15.01.041

Kuivenhoven M, Mason K. Arsenic (Arsine) Toxicity. StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2020 Jan. https://www.ncbi.nlm.nih.gov/ books/NBK541125/ Bookshelf ID: NBK541125; PMID: 31082169

Rahman MM, Chowdhury UK, Mukherjee SC, Mondal BK, Paul K, Lodh D, Biswas BK, Chanda CR, Basu GK, Saha KC, Roy S. Chronic arsenic toxicity in Bangladesh and West Bengal, India-a review and commentary. Journal of Toxicology: Clinical Toxicology. 2001; 39(7) :683–700. PMid:11778666. https://doi.org/10.1081/CLT100108509

World Health Organization (WHO). Guideline for drinking-water quality, 3rd edition (Recommendations). World Health Organization, Geneva; 2004.

Agency for Toxic Substances and Disease Registry (ATSDR). Toxicological Profile for Arsenic. Atlanta, GA: U.S. Department of Health and Human Services, Public Health Services; 2007.

Opresko DM. Risk assessment information system database, Oak Ridge Reservation Environmental Restoration Program, 1992. http://risk.lsd.ornl.gov/tox/ profiles/arsenic.shtml)

Kim HS, Kim YJ, Seo YR. An overview of carcinogenic heavy metal: Molecular toxicity mechanism and prevention. Journal of Cancer Prevention, 2015; 20(4):232. PMid:26734585 PMCid:PMC4699750. https://doi.org/10.15430/JCP.2015.20.4.232

Ajees AA, Marapakala K, Packianathan C, Sankaran B, Rosen BP. Structure of an As (III) S-adenosylmethionine methyltransferase: Insights into the mechanism of arsenic biotransformation. Biochemistry. 2012; 51(27):5476–85. PMid:22712827 PMCid:PMC3447999.


Jaishankar M, Tseten T, Anbalagan N, Mathew BB, Beeregowda KN. Toxicity, mechanism and health effects of some heavy metals. Interdisciplinary toxicology. 2014; 7(2):60–72. PMid:26109881 PMCid:PMC4427717. https://doi.org/10.2478/intox-2014-0009

Garbinski LD, Rosen BP, Chen J. Pathways of arsenic uptake and efflux. Environment International. 2019; 126:585–97. PMid:30852446 PMCid:PMC6472914. https://doi.org/10.1016/j.envint.2019.02.058

Grandjean P, Herz KT. Trace elements as paradigms of developmental neurotoxicants: Lead, methylmercury and arsenic. Journal of Trace Elements in Medicine and Biology. 2015; 31:130–4. PMid:25175507 PMCid: PMC4321972. https://doi.org/10.1016/j.jtemb.2014.07.023

Neha C, Pandey SK, Devendra M. Determination of arsenic content in the water and blood samples of ballia region using hydride generation atomic absorption spectrophotometer. Research Journal of Forensic Sciences. 2013; 1(4):1–3.

Jaiswal AK, Das S, Kumar V, Gupta M, Singh N. Simultaneous determination of zinc (Zn), cadmium (Cd), lead (Pb) and copper (Cu) in blood using differential-pulse anodic-stripping voltammetry. International Journal of Engineering Research. 2015; 4(5):235–9. https://doi.org/10.17950/ijer/v4s5/505

Jaiswal AK, Mali BU. Differential pulse cathodic stripping voltammetric determination of trace level of arsenic in blood and urine of a poisoned patient: A case study. Indian Journal of Forensic Medicine and Pathology. 2014; 7(4):153.



How to Cite

Mehta, K., Kanojia, R., Jaiswal, A. K., & Millo, T. (2022). Voltammetric Approach of Arsenic (Total) Determination in Blood using Sctrace Gold Electrode. Toxicology International, 27(3&4), 211–219. https://doi.org/10.18311/ti/2020/v27i3&4/26046



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