Acute, Sub-Acute and Genotoxicity Assessment of Cocculus hirsutus Hydroalcoholic Leaf Extract in Wistar Rats


Affiliations

  • Koneru Lakshmaiah Education Foundation, College of Pharmacy, Vaddeswaram, Andhra Pradesh, 522502, India
  • Vignan Institute of Pharmaceutical Technology, Visakhapatnam, Andhra Pradesh, 534202, India

Abstract

Acute (0, 500, 1000 and 2000 mg/kg) and 28-day (0, 100, 200 and 400 mg/kg) toxicity assessments of Cocculus hirsutus hydroalcoholic leaf extract (CH-Ex) were performed in Wistar rats consistent with OECD guidelines 423 and 407 respectively. Average body weights were recorded, blood and biochemical parameters were analyzed and histopathological studies were performed. At doses (12.5–100 μg/ml), genotoxicity assessments were conducted on pUC 19 plasmid by evaluating DNA damage protection. Acute toxicity assessment categorized CH-Ex as safe and non-toxic (category 5). The 28-day toxicity assessments showed no significant (p>0.05) changes in body weights, hematology and histopathology. At 400 mg/kg females exhibited a slight decline in SGPT, creatinine and BUN levels, while male rats exhibited a slight enhancement of SGOT and ALP levels (p<0.05). CH-Ex exhibited strong ability to protect DNA damage from oxidative stress in a dose-dependent manner.

Keywords

Biochemical, Cocculus hirsutus, DNA Damage, Histopathological, Oxidative Stress, Toxicity

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