Evaluation of Mutagenic Potential of Ethanolic Extract of Leaves of Couroupita guianensis using Salmonella typhimurium Tester Strains

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Authors

  • Rajendra M. Nagane
     Department of Toxicology (Mutagenicity), Jai Research Foundation, Valvada – 396105, Gujarat ,IN
  • Karishma R. Desai
     Department of Toxicology (Mutagenicity), Jai Research Foundation, Valvada – 396105, Gujarat ,IN
  • Indrajitsinh M. Barad
     Department of Toxicology (Mutagenicity), Jai Research Foundation, Valvada – 396105, Gujarat ,IN
  • Manish V. Patel
     Department of Toxicology (Mutagenicity), Jai Research Foundation, Valvada – 396105, Gujarat ,IN
  • Urjita V. Sheth
     C. G.Bhakta Institute of Biotechnology, Uka Tarsadia University (UTU), Maliba Campus, Bardoli – 394350, Gujarat ,IN
  • Kathirvelu Baskar
     Department of Ecotoxicology, Jai Research Foundation, Valvada – 396105, Gujarat ,IN
  • Bindi S. Patel
     Department of Toxicology (Mutagenicity), Jai Research Foundation, Valvada – 396105, Gujarat ,IN
  • Diwakar K. Shukla
     Department of Toxicology (Mutagenicity), Jai Research Foundation, Valvada – 396105, Gujarat ,IN
  • R. Krishnamurthy
     C. G. Bhakta Institute of Biotechnology, Uka Tarsadia University (UTU), Maliba Campus, Bardoli – 394350, Gujarat ,IN

DOI:

https://doi.org/10.18311/ti/2021/v28i3/27432

Keywords:

Reverse Mutation, Bacteria Strains, Couroupita guianensis

Abstract

India has a golden resource and well-recorded traditionally practised incomparable awareness of herbal medicine. Not all herbals are considered safe as minimal data is available concerning the safety of Couroupita guianensis to cause mutations. Considering the enormous therapeutic potential and the absence of specific safety data, we planned this research to provide data on the mutagenic potential of Couroupita guianensis. Therefore, the present study was conducted with ethanolic extract of leaves of Couroupita guianensis using Salmonella typhimurium tester strains TA1537, TA1535, TA98, TA100, and TA102 at different concentrations (viz., 156.25, 312.5, 625, 1250, 2500, and 5000 μg/plate) along with the negative control and respective positive controls. The methodology followed was the standard plate incorporation method as per the internationally approved OECD TG 471 guidelines. The study was performed with and without S9 mix using the plate incorporation method. Results were evaluated after meeting the acceptance criteria for the study. The extracts did not show mutagenicity when compared to the negative control (spontaneous mutation) and respective positive controls. There was no concentration-response increase of revertant colonies in treatments from any strain. This is the first of its kind of research having been conducted following all the internationally set guidelines and evidenced the safety of the plant material. Therefore, it is concluded that ethanolic extract of leaves of Couroupita guianensis did not show mutagenic potential in bacterial reverse mutation test with and without S9 mix.

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Published

2021-08-23

How to Cite

Nagane, R. M., Desai, K. R., Barad, I. M., Patel, M. V., Sheth, U. V., Baskar, K., Patel, B. S., Shukla, D. K., & Krishnamurthy, R. (2021). Evaluation of Mutagenic Potential of Ethanolic Extract of Leaves of <i>Couroupita guianensis</i> using <i>Salmonella typhimurium</i> Tester Strains. Toxicology International, 28(3), 255–265. https://doi.org/10.18311/ti/2021/v28i3/27432

Issue

Volume 28, Issue 3, July-September 2021

Section

Original Research
Crossref
Scopus
Google Scholar
Europe PMC
Received 2021-03-27
Accepted 2021-04-06
Published 2021-08-23

 

References

Idu M, Onyibe HI, Timothy O, Erhabor JO. Ethnomedicinal flora of Otuo people of Edo State, Nigeria. Asian J Plant Sci. 2008; 7(1):8212. https://doi. org/10.3923/ajps.2008.8.12 DOI: https://doi.org/10.3923/ajps.2008.8.12

Plewa MJ, Wagner ED. Activation of promutagens by green plants. Annu Rev Genet. 1993; 27:93–113. https://doi.org/10.1146/annurev.ge.27.120193.000521. PMid:8122914 DOI: https://doi.org/10.1146/annurev.ge.27.120193.000521

Mattana CM, Cangiano MA, Alcaráz LE, et al. Evaluation of cytotoxicity and genotoxicity of acacia aroma leaf extracts. Sci World J. 2014; 2014. https://doi. org/10.1155/2014/380850. PMid:25530999. PMCid: PMC4228826 DOI: https://doi.org/10.1155/2014/380850

Gothai S, Vijayarathna S, Chen Y, et al. In vitro and in vivo-scientific evaluation on cytotoxicity and genotoxicity of traditional medicinal plant Couroupita guianensis aubl. flower. Pharmacologyonline. 2019; 2:24–38.

de Queiroz FM, de Oliveira Matias KW, da Cunha MMF, Schwarz A. Evaluation of (anti)genotoxic activities of Phyllanthus niruri L. in rat bone marrow using the micronucleus test. Brazilian J Pharm Sci. 2013; 49(1):137–48. https://doi.org/10.1590/S1984-82502013000100015 DOI: https://doi.org/10.1590/S1984-82502013000100015

Sheba LA, Anuradha V. An updated review on Couroupita guianensis Aubl: A sacred plant of India with myriad medicinal properties. J Herbmed Pharmacol. 2020; 9(1):1–11. https://doi.org/10.15171/jhp.2020.01 DOI: https://doi.org/10.15171/jhp.2020.01

Elumalai A, Eswaraiah M, Didala A. Investigations on anti-oxidant, anti-arthritic and anti-platelet studies in Couroupita guianensis Aubl leaves by in-vitro methods. Pharma Sci Monit. 2012;3(3):2262-2269.

Umachigi SP, Jayaveera KN, Kumar CKA, Kumar GS. Antimicrobial, wound healing and antioxidant potential of Couroupita guianensis in rats. Pharmacologyonline. 2007; 3:269–81.

Khan MR, Kihara M, Omoloso AD. Antibiotic activity of Couroupita guianensis. J Herbs, Spices Med Plants. 2003; 10(3):95–108. https://doi.org/10.1300/ J044v10n03_10 DOI: https://doi.org/10.1300/J044v10n03_10

Kavitha R, Kamalakannan P, Deepa T, Elamathi R, Sridhar S, Suresh JK. In vitro antimicrobial activity and phytochemical analysis of Indian medicinal plant Couroupita guianensis aubl. J Chem Pharm Res. 2011; 3(6):115–21.

Sivakumar T, Shankar T, Geetha G. Efficacy of Couroupita guianensis against selected human pathogens. Adv Biol Res (Rennes). 2012; 6(2):59–63.

Prabhu V, Ravi S. Quantification of quercetin and stigmasterol of Couroupita guianensis aubl by HPTLC method and in-vitro cytototoxic activity by MTT assay of the methanol extract against HeLa, NIH 3T3 and HepG2 cancer cell lines. Int J Pharm Pharm Sci. 2012; 4(4):126–30.

Baskar K, Ignacimuthu S, Jayakumar M. Toxic effects of Couroupita guianensis against Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). Neotrop Entomol. 2015; 44(1):84–91. https://doi.org/10.1007/ s13744-014-0260-7. PMid:26013016 DOI: https://doi.org/10.1007/s13744-014-0260-7

Maheswaran R, Baskar K, Ignacimuthu S, Packiam SM, Rajapandiyan K. Bioactivity of Couroupita guianensis Aubl. against filarial and dengue vectors and nontarget fish. South African J Bot. 2019; 125:46–53. https://doi.org/10.1016/j.sajb.2019.06.020 DOI: https://doi.org/10.1016/j.sajb.2019.06.020

Otabe A, Ohta F, Takumi A, Lynch B. Mutagenicity and genotoxicity studies of aspartame. Regul Toxicol Pharmacol. 2019; 103:345–51. https://doi. org/10.1016/j.yrtph.2018.01.023. PMid:29408486 DOI: https://doi.org/10.1016/j.yrtph.2018.01.023

Han EH, Lim MK, Lee SH, Rahman MM, Lim YH. An oral toxicity test in rats and a genotoxicity study of extracts from the stems of Opuntia ficus-indica var. saboten. BMC Complement Altern Med. 2019; 19(1). https://doi.org/10.1186/s12906-019-2442-7. PMid:30691445. PMCid:PMC6350306 DOI: https://doi.org/10.1186/s12906-019-2442-7

Reddeman RA, Glávits R, Endres JR, et al. A toxicological evaluation of Germanium sesquioxide (Organic Germanium). J Toxicol. 2020; 2020. https:// doi.org/10.1155/2020/6275625. PMid:32322266. PMCid:PMC7160733 DOI: https://doi.org/10.1155/2020/6275625

Dodda S, Alluri VK, Golakoti T, Sengupta K. Acute, subacute, and genotoxicity assessments of a proprietary blend of Garcinia mangostana fruit rind and Cinnamomum tamala leaf extracts (CinDura®). J Toxicol. 2020; 2020. https://doi.org/10.1155/2020/1435891. PMid:32802054. PMCid: PMC7414347 DOI: https://doi.org/10.1155/2020/1435891

Dantas FG da S, Castilho PF de, Almeida-Apolonio AA de, Araújo RP de, Oliveira KMP de. Mutagenic potential of medicinal plants evaluated by the Ames Salmonella/microsome assay: A systematic review. Mutat Res - Rev Mutat Res. 2020;786. https://doi.org/10.1016/j.mrrev.2020.108338. PMid:33339578 DOI: https://doi.org/10.1016/j.mrrev.2020.108338

Al-Anazi KM, Al-Mareed AA, Farah MA, Ali MA, Hailan WAQ, Al-Hemaid FM. Protective effect of Capparis spinosa Extract against potassium bromate induced oxidative stress and genotoxicity in mice. Evidence-based Complement Altern Med. 2021;2021. https://doi.org/10.1155/2021/8875238. PMid:33531925. PMCid:PMC7837764 DOI: https://doi.org/10.1155/2021/8875238

Wang YH. Traditional uses, chemical constituents, pharmacological activities, and toxicological effects of Dendrobium leaves: A review. J Ethnopharmacol. 2021;270. https://doi.org/10.1016/j.jep.2021.113851. PMid:33485987 DOI: https://doi.org/10.1016/j.jep.2021.113851

Gupta VH, Gunjal MA, Wankhede SS, Deshmukh VS. Neuropharmacological evaluation of the methanolic extract of Couroupita guianensis Aubl. Flower in Mice. Int J Pharm Pharmacol Res. 2012; 1(5):242–6.

Elumalai A, Bargavi K, Krishna S, And Eswaraiah MC. Evaluation of anti-oxidant and hepatoprotective activity of Couroupita guianensis Leaves. J Cell Tissue Res. 2013; 13(2):3745–8.

Organ Econ Co-operation Dev. OECD Test No. 471: Bacterial reverse mutation test[Internet]. 1997. https://doi.org/10.1787/9789264071247-en

De Flora S, Izzotti A, Randerath K, et al. DNA adducts and chronic degenerative diseases. Pathogenetic relevance and implications in preventive medicine. Mutat Res - Rev Genet Toxicol. 1996;366(3). https://doi.org/10.1016/S0165-1110(96)00043-7 DOI: https://doi.org/10.1016/S0165-1110(96)00043-7

Andreassi MG, Botto N, Colombo MG, Biagini A, Clerico A. Genetic instability and atherosclerosis: Can somatic mutations account for the development of cardiovascular diseases? Environ Mol Mutagen. 2000;35(4). https://doi.org/10.1002/1098-2280(2000) 35:4<265::AID-EM1>3.0.CO;2-M DOI: https://doi.org/10.1002/1098-2280(2000)35:4<265::AID-EM1>3.0.CO;2-M

Aruoma OI. Methodological considerations for characterizing potential antioxidant actions of bioactive components in plant foods. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 2003; 523–4. https://doi.org/10.1016/S0027-5107(02)00317-2 DOI: https://doi.org/10.1016/S0027-5107(02)00317-2

Ross CA, Margolis RL. Neurogenetics: Insights into degenerative diseases and approaches to schizophrenia. Clin Neurosci Res. 2005;5(1 SPEC. ISS.). https://doi.org/10.1016/j.cnr.2005.07.001 DOI: https://doi.org/10.1016/j.cnr.2005.07.001

Test No. 471: Bacterial Reverse Mutation Test. OECD; 2020. https://doi.org/10.1787/9789264071247-en DOI: https://doi.org/10.1787/9789264071247-en

Sánchez F, Radaeva TV, Nikonenko BV, et al. Multigenic control of disease severity after virulent Mycobacterium tuberculosis infection in mice. Infect Immun. 2003;71(1). https://doi.org/10.1128/IAI.71.1.126-131. 2003. PMid:12496157. PMCid:PMC143141 DOI: https://doi.org/10.1128/IAI.71.1.126-131.2003

Food. International Conference on Harmonisation. Guidance on S2(R1) genotoxicity testing and data interpretation for pharmaceuticals intended for human use; availability. Notice [Internet].2012.

Johara FT, Aovi FI. Pharmacological screening of in vitro activity of methanol extract of nagalinga flower. Pharmacologyonline. 2020;2.

George SA, Bhadran S, Sudhakar M, Harini BP. Comprehensive in vitro evaluation of pharmacological activities of selected mass spectrometry profiling of Flacourtia jangomas flower extract. Asian J Pharm Clin Res. 2017; 10(5):237–44. https://doi.org/10.22159/ajpcr.2017.v10i5.17419 DOI: https://doi.org/10.22159/ajpcr.2017.v10i5.17419

Begum K, Shammi M, Hasan N, Asaduzzaman M, Appiah KS, Fujii Y. Potential allelopathic candidates for land use and possible sustainable weed management in South Asian ecosystem. Sustain. 2019;11(9). https://doi.org/10.3390/su11092649 DOI: https://doi.org/10.3390/su11092649

Gupta VH, Wankhede SS, Gunjal MA, Juvekar AR. Antidepressant like effect of Couroupita guianensis Aubl. flowers in animal model of depression. Int J Toxicol Pharmacol Res. 2012; 4(2):12–16.

Pinheiro MMG, Fernandes SBO, Fingolo CE, Boylan F, Fernandes PD. Anti-inflammatory activity of ethanol extract and fractions from Couroupita guianensis Aublet leaves. J Ethnopharmacol. 2013; 146(1):324–30. https://doi.org/10.1016/j.jep.2012.12.053. PMid: 23313951 DOI: https://doi.org/10.1016/j.jep.2012.12.053

Edziri H, Mastouri M, Mahjoub A, et al. Toxic and mutagenic properties of extracts from Tunisian traditional medicinal plants investigated by the neutral red uptake, VITOTOX and alkaline comet assays. South African J Bot. 2011; 77(3):703–10. https://doi.org/10.1016/j.sajb.2011.03.007 DOI: https://doi.org/10.1016/j.sajb.2011.03.007

Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Investig Suppl. 1968; 97:77–89.

Pradhan D, Panda PK, Tripathy G. Evaluation of the immunomodulatory activity of the methanolic extract of Couroupita guianensis aubl. Flowers in rats. Nat Prod Radiance. 2009; 8(1):37–42.

Moosavi M, Jalali A, Siahpoosh A, Farajzadeh-Shikh A, Kianipur F. Assessing mutagenicity of methanolic exteract of Abrus precatorius seeds using ames bioassay. J Med Sci. 2013; 13(2):118–23. https://doi.org/10.3923/jms.2013.118.123 DOI: https://doi.org/10.3923/jms.2013.118.123

Lee JS, Cho JH, Lee DS, Son CG. Genotoxicity evaluation of an ethanol extract mixture of Astragali radix and Salviae miltiorrhizae Radix. Evidence-based Complement Altern Med. 2018; 2018. https://doi.org/10.1155/2018/5684805. PMid:30402128. PMCid: PMC6198562 DOI: https://doi.org/10.1155/2018/5684805

Sharififar F, Dehghan-Noudeh G, Moshafi M, et al. Antimutagenic activity of major fractions of Zataria multiflora Boiss by Ames method. Asian J Pharm. 2015; 9(3):195–9. https://doi.org/10.4103/0973-8398.160316 DOI: https://doi.org/10.4103/0973-8398.160316

Abdul-Hafeez EY, Karamova NS, Ilinskaya ON. Evaluation of mutagenic and antimutagenic potential of stem bark aqueous extracts of eight trees by the bacterial reverse mutation assay. Ecol Genet. 2018; 16(3):55–61. https://doi.org/10.17816/ecogen16355-61 DOI: https://doi.org/10.17816/ecogen16355-61

EFSA. Guidance for submission for food additive evaluations. EFSA J. 2012;10(7). https://doi.org/10.29 03/j.efsa.2012.2760 DOI: https://doi.org/10.2903/j.efsa.2012.2760

Hardy A, Benford D, Halldorsson T, et al. Clarification of some aspects related to genotoxicity assessment. EFSA J. 2017; 15(12):5113. https://doi.org/10.2903/j. efsa.2017.5113. PMid:32625393 DOI: https://doi.org/10.2903/j.efsa.2017.5113

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