Modulatory effect of vitamin E and C on SO^ induced hematological alterations in albino rats


Affiliations

  • Department of Zoology, School of Life Sciences, Khandari Campus, Dr. B.R.AmbedkarUniversity, Agra, U.P, 282002, India

Abstract

The present study aimed to investigate the alterations in selected hematological parameters (total erythrocyte count, total leucocyte count, hemoglobin concentration, packed cell volume, erythrocyte sedimentation rate and red cell indices(MC V, MCH, MCHC) of wistar albino rats after short term (80 ppm, 1 h/ d for 28 days) exposure to SO ^ gas and protective effect of vitamin E (2.5mg/rat) andC (5mg/rat) individually and In combination. The results of the present study imply that hematological parameters altered after SO^ gas inhalation and lead to reduction in TEC, Hb.Conc, PCVand red cell indices, while elevation in TLC and ESR. However, SO^ induced hematological alterations modulated after supplementation of vitamin E and C individually and in combination due to antioxidant defense mechanism against toxic action of SO^ inhalation. The findings of the present study exhibit that the supplementation of vitamin E and C in combination have more potential to mitigate SO^ induced hematological alterations in albino rats than the supplementation of vitamin E and C individually.

Keywords

SO gas. Hematological alterations. Albino rats, Vitamin E and C.

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References

Andersson, E., Nilsson, T., Persson, B., Wingren, G. and Toren, K. (1998) Mortality from asthma and cancer among sulfite mill workers. Scand. J. Work. Environ. HIth., 24, 12-17.

Baskurt, O.K. (1988) Acute haematologic and haemorrheologic effects of sulphur dioxide inhalation. Arch. Environ. Heaith, 43, 344-348.

Dacie, J.v. and Lewis, S.M. (1969) Practical haematology (5'"ed.) J. and A. Charch Hill, Livingstone, Edinburgh.

EtIik, 0., Tomur, A., Kutman, M.N., Yorukan, S. and Duman, O.(1995) The effects of sulphur dioxide inhalation and antioxidant vitamins on red blood cell lipoperoxidation.Environ. Res., 71, 25-8.

EtIik, O., Tomur,A.,Tuncer, M., Ridvanagaoglu, A.Y. and Andac, O.(1997) Protective effect of antioxidant vitamins or red blood cell lipoperoxidation induced by SO^ inhalation. J. Basic Clin.Phystol. Phannacol., 8, 31-43.

Goniz, A., Llacuna, S., Riera, M., Riera, J. and Nadal, J. (1996) Effects of air pollution on hematological and plasma parameters In Apodemus sylvaticus and Mus masculus. Arch. Environ.Contam. Toxicol., 31, 153-158.

Guyton, C. and Hall, J.E. (2002) Textbook of Medical Physiology 10" ed. Sounders College Publishing, Philadelphia, 1064 pp.

Haider, S.S. (1985) Effects of exhaust pollutant sulphur dioxide on lipid metabolism of guinea pig organs. Ind. Health, 23, 81-87.

Halliwell, B.,Aeschbach, R., Loliger, J. and Aruoma, 0.1. (1995) The characterization of antioxidants. FoodChem. Toxicol., 33, 601-617.

Jain, S.K. (1989) Hyperglycemia can cause membrane lipid peroxidation and osmotic fragility in human red blood cells. J. Biol. Chem., 264, 21340-21345.

Lal, K., Dutta, K.K., Vachhrayani, K.D. and Gupta, G.S.D. (1993) Histomorphological changes in lungs of rats following exposure to wood smoke. Ind. J. Exp. Biol., 31, 761-764.

Mukherjee, K.L. (1997) Bleeding disorders. Medical laboratory technology I'' ed. Tata Mc Graw-Hill publishing company limited, New Delhi., pp 481.

Pant, M.C. (2004) Essentials of Biochemistry (9'" ed). Kedarnath Ramnath and Co. Meerut, pp 670.

Regnault, C, Postaire, ERR., Rousset, G.J.P., Bejot, M. and Hazebroucg, G.F. (1993) Influence of beta carotene, vitamin E, and vitamin C on endogenous antioxidant defenses in erythrocytes. Ann. Parmacothe, 27,13491350.

Sapakal, V.D., Shikalgar, TS., Ghadge, R.V., Adnaik, R.S., Naikwade, N.S. and Magdum, C.S. (2008) In vivo screening of antioxidant profile: A Review. J. Herbal Med. Toxicol., 2,1-8.

Senturk, U.K., Yalcin, C , Gunduz, F., Kuru, 0., Meiselman, H.J.and Baskurt, O.K. (2005) Effect of antioxidant vitamin treatment on the time course of hematological and hemorheological alterations after an exhausting exercise episode in human subjects. J. Appl. Physiol., 98, 1272-1279.

Smith, J.A., Kolbouch-Braddon, M., Gillam, I., Telford, R.D. and Weidemann, M.J. (1995) Changes in the susceptibility of red blood cells to oxidative and osmotic stress following submaximal exercise. Eur J. Appl. Physiol., 70, 427-436.

Tewari, A. and Shukia, N.P. (1991) Air pollution adverse effects of sulphur dioxide. Rev. Environ. HIth., 9, 39-46.

Toker, K., Ozer, N.K., Yalcin, AS., Tuzuner, S., Gogus, FY. and Emerk, K. (1990) Effect of chronic halothane exposure on lipid peroxidation, osmotic fragility and morphology of rat erythrocytes. J. Appl. Toxicol., 10, 407-409.

Wintrobe, MM., Lee, G.R., Boggs, D.R., Bithell, T C , Foerster, J., Athens, J.W. and Lukens, J.N. (1981) Clinical hematology, (8'" ed). Lea & Febiger, Philadelphia, pp 1882.


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