Journal of Biological Control

Prevalence and molecular characterization of viruses causing diseases in Bombyx mori L. (Lepidoptera: Bombycidae) from different climatic regions of India


Affiliations

  • Central Silk Board, Pampore, Central Sericultural Research and Training Institute, Ministry of Textiles, Government of India, Jammu and Kashmir, 192 121, India
  • Central Silk Board, Mysore, Central Sericultural Research and Training Institute, Ministry of Textiles, Government of India, Karnataka, 570 008, India
  • Central Silk Board, Mysore, Central Sericultural Research & Training Institute, Karnataka, 570 008, India

Abstract

A number of viruses are known to cause the disease in mulberry silkworm, Bombyx mori L. and cause significant cocoon crop loss to the farmers. The study has been undertaken to assess the prevalence of silkworm viral diseases caused by B. Mori Nucleopolyhedrovirus (BmNPV), B. mori Infectious Flacherie Virus (BmIFV) and B. mori Densovirus1 (BmDNV1) through external symptoms and molecular characterization from different climatic regions of India. During the intensive exploratory surveys in 2017, silkworm larvae with typical symptoms of BmNPV, BmIFV and BmDNV1 were collected across seven provinces from North India [Kashmir (temperate), Jammu, Ghumarwin in Himachal Pradesh, Dehradun in Uttarakhand (subtropical) and South India [Karnataka, Andhra Pradesh and Tamil Nadu (tropical)]. Dissection of diseased specimens confirmed the presence of virus through anatomical changes viz, size, shape and colour of organs after disease attack. The viruses were isolated and identified through PCR amplification of highly conserved genes. The results reveal that BmNPV, BmIFV and BmDNV1 are evenly prevalent across India. The infection percentage of BmNPV, BmIFV and BmDNV1 in North India (11.41 ± 1.21, 7.81 ± 0.67 and 7.40 ± 0.61) was significantly higher than South India (1.61 ± 0.17, 1.52 ± 0.14 and 1.62 ± 0.14), respectively. The highest prevalence of these viruses was observed from subtropical followed by temperate and tropical climate. The knowledge of the prevalence of these viruses in India and their synergism with bacteria and influence of other possible factors is important for preventing cocoon crop losses caused by viral diseases in India.

Keywords

Bombyx mori, BmNPV, BmIFV, BmDNV1, characterization, prevalence

Subject Discipline

Entomology; Sericulture; Silkworm Pathology

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References

Gani M, Chouhan S, Babulal, Gupta RK, Khan G, Kumar NB, Saini P, Ghosh MK. 2017. Bombyx mori nucleopolyhedrovirus (BmNPV): its impact on silkworm rearing and management strategies. J Biol Control 31(3): 119–122. https://doi.org/10.18311/jbc/2017/16269

Gupta RK, Gani M, Jasrotia P, Srivastava K. 2013. Development of the predator Eocanthecona furcellata on different proportions of nucleopolyhedrovirus infected Spodoptera litura larvae and potential for predator dissemination of virus in the field. BioControl 58(4): 543–552. https://doi.org/10.1007/s10526-013-9515-1

Illahi I, Nataraju B. 2007. Prevalence of nuclear polyhedrosis in mulberry silkworm, Bombyx mori L. in Jammu and Kashmir. Indian J Seric. 46(1): 43–4

Jehle JA, Lange M, Wang H, Hu Z, Wang Y, Hauschild, R. 2006. Molecular identification and phylogenetic analysis of baculoviruses from Lepidoptera. Virology 346: 180–193. doi: PMid: 16313938 https://doi.org/10.1016/j.virol.2005.10.032

Khurad AM, Kanginakudru S, Qureshi SO, Rathod MK, Rai MM. 2006. A new Bombyx mori larval ovarian cell line highly susceptible to nucleopolyhedrovirus. J Invertebr Pathol. 92: 59–65. PMid: 16713602 https://doi.org/10.1016/j.jip.2006.03.005

Lange M, Wang H, Zhihong H, Jehle JA. 2004. Towards a molecular identification and classification system of lepidopteran-specific baculoviruses. Virology 325(1): 36–47. PMid: 15231384 https://doi.org/10.1016/j.virol.2004.04.023

Luo Y, Nartker S, Miller H, Hochhalter D, Wiederoder M, Wiederoder S, Setterington E, Drzal LT, Alocilja EC. 2010. Surface functionalization of electrospun nanofibers for detecting Escherichia coli O157:H7 and BVDV cells in a direct–charge transfer biosensor. Biosens Bioelectron. 26: 1612–1617. doi: 10.1016/j.bios.2010.08.028 PMid: 20833013

Nataraju B, Sivaprasad V, Datta RK, Gupta SK, Shamim M. 1994. Colloidal textile dye-based dipstick immunoassay for the detection of nuclear polyhedrosis virus (BmNPV) of silkworm, Bombyx mori L. J Invertebr Pathol. 63(2): 135–139. https://doi.org/10.1006/jipa.1994.1026

O’Reilly DR, Miller LK, Luckow VA. 1992. Baculovirus expression vectors: A laboratory manual. W. H. Freeman & Co., New York. https://doi.org/10.1016/00928674(93)90288-2

Palhan VB, Gopinathan KP. 1996. Characterization of a local isolate of Bombyx mori nuclear polyhedrosis virus. Curr Sci. 70(2): 147–153.https://www.jstor.org/ stable/24096983

Ravikumar G, Urs SR, Prakash NV, Rao CGP, Vardhana KV. 2011. Development of a multiplex polymerase chain reaction for the simultaneous detection of microsporidians, nucleopolyhedrovirus, and densovirus infecting silkworms. J Invertebr Pathol. 107(3): 193–197. PMid: 21570404 https://doi.org/10.1016/j.jip.2011.04.009

Reddy BK, Rao JVK. 2009. Seasonal occurrence and control of silkworm diseases, grasserie, flacherie and muscardine and insect pest, Uzi fly in Andhra Pradesh, India. Int J Indus Entomol. 18(2): 57–61.

Savanurmath CJ, Basavarajappa S, Hinchigeri SB, Ingalhalli SS, Singh KK, Sanakal RD. 1992. Relative incidence of silkworm viral disease in agroclimatic zones of northern Karnataka, India. National conference on mulberry sericulture research, CSR & TI, Mysore, Dec. p. 123.

Sivaprasad V. Chandrasekharaiah C. Misra S. Kumar KPK. Rao YUM. 2003. Screening of silkworm breeds for tolerance to Bombyx mori nuclear polyhedrosis virus (BmNPV). Int J Indus Entomol. 7: 87–91.

Tang FB, Zhanga Y, Shaoa Y, Zhua F, Huanga P, Baia X. 2017. Isolation and identification of a new Bombyx mori nucleopolyhedrovirus strain isolated from Yunnan, China. Science Asia 43: 26–32. https://doi.org/10.2306/scienceasia1513-1874.2017.43.026

Vootla SK, Lu XM, Kari N, Gadwala M, Lu Q. 2013. Rapid detection of infectious flacherie virus of the silkworm, Bombyx mori, using RT-PCR and nested PCR. J Insect Sci. 13(1): 120. PMid: 24785655 https://doi.org/10.1673/031.013.12001


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