Analysis of bioactive minor compounds in three olive oils from varieties of olive tree eastern Algerian (Bouricha, Limli and Blanquette).


  • Faculty of Science, Department of Agronomy,El Tarf University, 36000, Algeria
  • Department of Biology, Faculty of Sciences, El Tarf University, 36000, Algeria
  • Department of Biochemistry, Faculty of Sciences, Badji-Mokhtar University, Annaba, 23000, Algeria


The study of bioactive compounds on the health of the unsaponifiable fraction of three olive oils from three cultivars of eastern Algeria carried out by gas chromatography revealed the presence of 11 compounds with the predominance of b-Sitosterol, Sterol characteristic of olive oils and having a nutritional and pharmacological value. The highest content was found in Limli oil (81.59%). Two triterpene dialcohols were chromatographed with sterols (erythrodiol and uvaol), the content of which can be identified fraud and to detect whether the virgin oil is mixed with the oil of pomace. Analysis of the alcohol fraction by GPC-FID revealed the predominance of a triterpene alcohol, 24-methylene cycloarenol for the three varieties with a higher content for Blanquette (48.03%) followed by cycloarenol. For the aliphatic alcohols, the analysis shows the predominance of four alcohols with 22, 23, 24 and 28 carbon atoms whose contents are higher for Limli oil. The spectrophotometric determination of antioxidant pigments shows that Limli oil is richer in chlorophylls with an average of 13.53 mg / kg and a significantly higher carotenoid content for Bouricha and Limli (13.10 -12.82 mg / Kg). The total polyphenols were lower for Blanquette (83.36 mg / kg) compared with Bouricha and Limli (133.3 mg / kg and 121.3 mg / kg), making them more stable during oxidation storage and whose beneficial effects of olive oil are mainly attributed to these substances, in particular the antioxidant action that would protect against diseases of oxidative stress.


Antioxidant, Bioactive Minor Components, Carotenoid, Olive Oils, Phytosterols

Subject Collection

East of algeria

Subject Discipline

biology: biochemistry; Nutrition ; pharmacology

Full Text:


Servili M, Selvaggini R, Esposto S, Taticchi A, Montedoro G. and Morozzi M. Health and sensory properties of virgin olive oil hydrophylic phenols: Agronomic and technological aspects of production that affect their occurrence in the oil. Journal of Chromatography A.2004; 1054(1-2):113–27.

Servili M, Montedoro G. Contribution of phenolic compound in virgin olive oil quality. European Journal of Lipid Science and Technology. 2002; 104:602–13. https://<602::AIDEJLT602> 3.0.CO;2-X

Cicerale S, Lucas L, Keast R. Biological activities of phenolic compounds present in Virgin Olive Oil. Int J Mol Sci. 2010; 11(2):458–79. PMid:20386648 PMCid:PMC2852848.

Phillips KM, Ruggio DM, Toivo JI, Swank MA. Simpkins AH. Free and esterified sterol composition of edible oils and fats. J Food Compos Anal. 2002; 15:123–42. https://

Boskou D, Blekas G, Tsimidou M. Olive oil composition.Boskou DD, editor. Olive Oil, Chemistry and Technology. 2nd ed. Champaign Illinois: American Oïl Chemists Society; 2006. p. 41–72. PMid:17110101.

Hendriks HF, Weststrate JA, van Vliet T, Meijer GW.Spreads enriched with three different levels of vegetable oil sterols and the degree of cholesterol lowering in normocholesterolaemic and mildly hypercholesterolaemic subjects. Eur J Clin Nutr. 1999; 53:319–27. PMid:10334658.

Herrera MD, Rodriguez-Rodriguez R, Ruiz-Gutierrez V.Fonctional properties of pentacyclic triterpenes contained in « Orujo » olive oil. Current Nutr Food Sci. 2006; 2:45–9.

Tekaya IB, Hassouna M. Étude de la stabilité oxydative de l’huile d’olive vierge extra tunisienne au cours de son stockage). OCL. 2005; 12(5-6):447–54.

Giuffrida D, Salvo F, Salvo A, La Pera L, Dugo G. Pigments composition in monovarietal virgin olive oils from various Sicilian olive varieties. Food Chemistry. 2007; 101:833–7.

Rebour H. Situation actuelle de l’oléiculture en Algérie.Alger. 2005; 46:1–6.

COI/OH/ Doc. Nº 1. Novembre 2011.Guide pour la détermination des caractéristiques des olives à huile.

COI/T.20/Doc. No 30/Rev. 1. Method of analysis determination of the composition and content of sterols and Triterpene dialcohols by Capillary column gas chromatography; 2013 Nov.

Lopez-Lopez A, Monta-o A, Ruiz-Mendez MV, GarridoFernandez A. Sterols, fatty alcohols, and triterpenic alcohols in commercial table olives. Journal of the American Oil Chemists Society. 2008; 85:253–62. https://

Montedoro G, Servili M, Baldioli M, Miniati E. Simple and hydrolyzable phenolic compounds in virgin olive oil. 2. Initial characterization of the hydrolyzable fraction. J Agric Food Chem. 1992; 40(9):1577–80.

Roncero AV, Del Valle CJ. Determination de la teneur en polyphenols totaux dans l’huile d’olive. Grasas y Aceites.1973; 24(6):350–7.

Wolff JP. Manuel d’analyse des corps gras. Paris: Azoulay.1968. p. 245.

Minguez MIM, Rejano L, Guandul B, Sanchez AH, Garido J. Color pigment, correlation in virgin olive oil. J Am Oil Chem. 1991; 68:332–6. BF02657688

Giuffre AM, Louadj L, Poiana M, Macario A. Sterol composition of oils extracted from olives cultivars of the province of Reggio Calabria (south of Italy). Riv Ital Delle Sostanze Grasse. 2012; 89(3):177–83.

Gracia S. Composicion quimica de distintas calidades de aceites de oliva virgen de la variedad “Empeltre” en el bajo Aragón. Grasas Aceites. 2001; 52(1):52–8.

Ranalli A, Pollastri L, Contento S, Di Loreto G, Iannucci E, Lucera L. Sterol and alcohol components of seeds, pulp, whole olive fruit oils. Their use to characterise olive fruit variety by multivariates. Journal of the Science of Food and Agriculture. 2002; 82:854–9. jsfa.1116

Sims RJ, Fioriti JA, Kanuk MJ. Sterol additives aspolymerization inhibitors for frying oils. J Am Oil Chem Soc. 1972; 49:298–301. BF02637578

Konuskan DB. Mungan B. Effects of variety, maturation and growing region on chemical properties, fatty acid and sterol compositions of virgin olive oils. J Am Oil Chem Soc. 2016; 93:1499–508.

Quillez J, Garcila-Lorda P, Salas-Salvadol J. Potential uses and benefits of phytosterols in diet: Present situation and future directions. Clin Nutr. 2003; 22:343–51.

Pardo JE, Cuesta MA, Alvarruiz A. Evaluation of potential and real quality of virgin olive oil from the designation of origin’Aceite Campo de Montiel (Ciudal Real,Spain).Food Chem. 2007; 100:977–84.

Alves MR, Cunha SC, Amaral JS, Pereira JA, Oliveira MB.Classification of PDO olive oils on the basis of their sterol composition by multivariate analysis. Analytica Chimica Acta. 2005; 549:166–78.

Temime SB, Manai H, Methenni K, Baccouri B, Abaza L, Daoud D, Casas JS, Bueno EO, Zarrouk M. Sterolic composition of Chéetoui virgin olive oil: Influence of geographical origin. Food Chem. 2008 ; 110:368–74. PMid:26049228.

Stiti N, Msallem M, Triki S, Cherif A. Etude de la fraction insaponifiable de l’huile d’olive de differentes varietes Tunisiennes. La Rivista Italiana delle Sostanze Grasse.2002; 79(10):357–63.

Gutierrez F, Varona I, Albi MA. Relation of acidity and sensory quality with sterol content of olive oil from stored fruit. J Agric Food Chem. 2000; 48:1106–10.PMid:10775357.

Gracia MS, Benavente-Garcia O, Castillo J, Lorente J, Ortuno A, Del Rio JA. Antioxidant activity of phenolics extracted from Olea europaea L leaves. Food Chemistry.2000; 68:457–62.

Ostlund RE Jr. Phytosterols in human nutrition. Annu Rev Nutr. 2002; 22:533–49. PMid:12055357.

Ambavade SD, Misar AV, Ambavade PD. Pharmacological, nutritional, and analytical aspects of β-sitosterol: A review.Oriental Pharmacy and Experimental Medicine. 2014; 14(3):193–211.

Homapour M, Ghavami M, Piravi-Vanak Z, Hosseini SE. Chemical properties of virgin olive oil from Iranian cultivars grown in the Fadak and Gilvan regions. Grasas Aceites. 2014; 65(4):e043. gya.0351141

Pardo JE, Cuesta MA, Alvarruiz A. Evaluation of potential and real quality of virgin olive oil from the designation of origin’ Aceite Campo de Montiel (Ciudal Real,Spain).Food Chem. 2007; 100:977–84.

Mariani C, Bellan G, Morchio G, Pellgrino A. Free and esterified minors compoments of olive and hazelnut: Their potential utilisation in checking oil blend. Rivista italiana delle sostanze grasse. 1999; 76(7/8):297–305.

Fuentes de Mendoza M, De Miguel Gordillo C, Expoxito JM, Sanchez Casas J, Cano MM, Vertedor DM, Baltasar MNF. Chemical composition of virgin olive oils according to the ripening in olives. Food Chemistry. 2013; 141:2575–81. PMid:23870997.

Sarolic M, Gugic M, Friganovic E, Giovanni Tuberoso CI, Jerkovic I. Phytochemicals and other characteristics of Croatian monovarietal extra virgin olive oils from Oblica, Lastovka and Levantinka varieties. Molecules. 2015; 20:4395–409. PMid:25759956. molecules20034395

Zegane O, Keciri S, Louaileche H. Physicochemical characteristics and pigment content of algerian olive oils: effect of olive cultivar and geographical origin.International Journal of Chemical and Biomolecular Science. 2015; 1(3):153–7.

Tanouti K, Serghini-Caid H, Chaieb E, Benali A, Harkous M, Elamrani A. Quality improvement of olive oils produced in the eastern Morocco. Les technologies de Laboratoire. 2011; 6(22):1–12.

Hoshina C, Tomita K, Shioi Y. Antioxidant activity of chlorophylls: its structure activity relationship.Photosynthesis: Mechanisms Effects. 1998; 4:3281–4.

Ferruzzi MG, Blakeslee J. Digestion, absorption, and cancer preventative activity of dietary chlorophyll derivatives. Nutrition Research. 2007; 27:1–12.

Hsu C-Y, Chao P-Y, Hu S-P, Yang CM. The antioxidant and free radical scavenging activities of chlorophylls and pheophytins. Food and Nutrition Sciences. 2013; 4:1–8.

Mayne ST. Beta-carotene, carotenoids, and disease prevention in humans. FASEB. 1996; 10:690–701.

Stahl W, Sies H. Bioactivity and protective effects of natural carotenoids. Biochimica et Biophysica Acta. 2005; 1740:101–7. PMid:15949675.

Benaziza A, Semad D. Oleiculture: Caracterisation de six variétés d’olives introduites dans le sud-est Algerien.European Scientific Journal Novembre. 2016; 12:537–53.

Garcia A, Brenes M, Garcia P, Romero C, Garrido A.Phenolic content of commercial olive oils. European Food Research and Technology. 2003; 216(6):520–5.

Bonanome A, Pagnan A, Caruso D, Toia A, Xamin A, Fedeli E, Berra B, Zamburlini A, Ursini F, Galli G. Evidence of postprandial absorption of olive oil phenols in humans.Nutrition Metabolism and Cardiovascular Diseases. 2000; 10:111–20. PMid:11006919.

Edwin N. Frankel. Nutritional and biological properties of extra virgin olive oil. J Agri Food Chem. 2011; 59(3):785– 92. PMid:21210703.

Covas MI, Nyyssonen K, Poulsen HE, Kaikkonen J, Zunft HJ, Kiesewetter H, Gaddi A, de la Torre R, Mursu J, Baumler H, Nascetti S, Salonen JT, Fito M, Virtanen J, Marrugat J, EUROLIVE Study Group. The effect of polyphenols in olive oil on heart disease risk factors: A randomized trial. Ann Intern Med. 2006; 145(5):333–41.

Edwin N. Frankel. Lipid oxidation. 2nd ed. University of California, USA: The Oily Press 2005. p. 486.

Hernaez A, Fernandez-Castillejo S, Farras M, Catalan U, Subirana I, Montes R, Sola R, Mu-oz-Aguayo D, Gelabert-Gorgues A, Diaz-Gil O, Nyyssonen K, Zunft HJ, De La Torre R, Martin-Pelaez S, Pedret A, Remaley AT, Covas MI, Fito M. Olive oil polyphenols enhance highdensity lipoprotein function in humans: a randomized controlled trial. Arterioscler Thromb Vasc Biol. 2014 Sep; 34(9):2115–9. PMid:25060792. ATVBAHA.114.303374


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