Evaluation of Wound Healing Effect of Eel Mucus Ointment (Belutidine) in Mice by Incision Model


Affiliations

  • Universitas Islam Sultan Agung, Faculty of Medicine, Research Institute, Department of Pharmacy, Indonesia

Abstract

Background: It has been reported by the Ministry of Health that the prevalence of wounded and worsening events is increasing in Indonesia. This situation requires more intensive attention. Eel mucus has been an alternative treatment of wounds in some parts of Indonesian society since ancient times. Aim and Objectives: To explore the effectiveness of eel mucus ointment as a natural wound healing product. Material and Methods: This experimental study used 45 mice divided into 3 groups; 15 mice in each group. All mice were aseptically wounded 1.5 cm on their anterior region of back skin using a sterile scalpel, and then treated with placebo, povidone iodine ointment, and with eel mucous ointment (Belutidine). On the third, sixth and ninth day, the mice were sacrificed. Data on wound length were taken on 9th day. Microscopic data of histologic slides collected were: number of neutrophil, mono-nuclear, neo-vascular and fibrosis cells. Data were analyzed using statistical analysis. Results: There was a significant difference in wound length between the groups (p = 0.008). The number of mono-nuclear cells on the third day was different significantly among the groups (p = 0.008). There was no significant difference of the overall observed cells on day 6 and there was a difference in mean number of neutrophils and fibrosis on day nine (p < 0.05). On day nine, the group treated with eel mucus ointment had the highest number of mean of neo-vascular and fibrosis cells. Conclusion: Eel mucous ointment is effective for wound healing.

Keywords

Eel Mucus Ointment, Fibrosis Cells, Mono-nuclear, Neo-vascular, Neutrophil, Wound Healing.

Full Text:

References

Hashemi SA, Madani SA, Abediankenari S. The review on properties of Aloe vera in healing of cutaneous wounds. BioMed Research International. 2015; 1–6. Crossref. PMid:26090436 PMCid:PMC4452276

Ministry of Health Republic Indonesia. Report on Basic Health Studies (RISKESDAS). 2013.

Rahmawati I. The different effects of wound care using Leucaena glauce benth and povidone iodine 10% in accelerating wound healing in Cavia pocellus. J Wiyata. 2014; 1:227–34.

Serafini MR, Guimaraes AG, Quintans-Junior LG, Nunes PS, Matos IG, Sravanan S, De Souza AAA. Recent patents on medicinal plants/natural products as a therapeutic approach to wounds and burns healing. Recent Pat Biotechnol. 2014;8:231–9. Crossref. PMid:27099146

Andrade PHM, Schmidt R E, Carollo CA, Rodrigues MML, Viana LH, De Souza SA, et al. Effect of powdered shells of the snail Megalobulimus lopesi on secondaryintention wound healing in an animal model. Evidencebased Complement Altern Med. 2015; 1–9.

Builders PF, Kabele-Toge B, Builders M, Chindo B, Anwunobi P, and Isimi Y. Wound healing potential of formulated extract from hibiscus sabdariffa calyx. Indian Journal of Pharmaceutical Sciences. 2013; 75(1):45–52. Crossref. PMid:23901160 PMCid:PMC3719149

Hajiaghaalipour F, Kanthimathi MS, Abdulla MA, and Sanusi J, The effect of camellia sinensis on wound healing potential in ananimal model. Evidence-Based Complementary and Alternative Medicine. 2013; 1–7. Crossref. PMid:23864889 PMCid:PMC3705756

Mulyani D, Febriyenti F, Almahdy A. The Effect of Monopterus albus extract on burns of Sprague-Dawley White Rats. Journal Sains Farmasi and Klinis, 2016; 2(2):191–4. Crossref.

Kumar S, Ghosh D, Biswas TK, Dutta U, Das P, Kundu S. Spermatheca gland extract of snail (Telescopium telescopium) has wound healing potential: an experimental study in rabbits. International Journal of Low Extrem Wounds. 2008; 7(4):204–9. Crossref. PMid:19019846

Harmon C, Hawkins J, Li J, Connell C, Miller M, Saenger M, et al. Effects of topical application of silver sulfadiazine cream, triple antimicrobial ointment, or hyperosmolar nanoemulsion on wound healing, bacterial load, and exuberant granulation tissue formation in bandaged fullthickness equine skin wounds. Am J Vet Res. 2017 May; 78(5):638–46.

Liang Y, Guan R, Huang W, Xu T. Isolation and identification of a novel inducible antibacterial peptide from the skin mucus of Japanese Eel, Anguilla japonica. Protein Journal. 2011; 30:413–21. Crossref. PMid:21796440

Divadi A, Yuliani S. Preparation and effectiveness test of scare-less wound gel of Anredera cordifolia extract and piroxicam. J Farm sains dan komunitas. 2015; 12(2):41–7.

Adibhesami M, Ahmadi M, Farshid AA, Sarrafzadehrezaei F, Dalir-naghadeh B. Effects of silver nanoparticles on Staphylococcus aureus contaminated open wounds healing in mice: An experimental study. Vet Res Forum.

; 8(1):23–8. PMid:28473893 PMCid:PMC5413307 14. Kumar V, Cotran R., Robbins SL. Robbins Basic Patholoy. 10th ed. Elsevier Inc. 2017. p.75-82.

Pastar I, Stojadinovic O, Yin NC, Ramirez H, Nusbaum AG, Sawaya A, et al. Epithelialization in Wound Healing: A Comprehensive Review. Advanced Wound Care 2014;3(7):445–64. Crossref. PMid:25032064 PMCid: PMC 4086220

Huang S, Lin CH, Chang KP, et al. Clinical Evaluation Comparing the Efficacy of Aquacel Ag with Vaseline Gauze versus 1% Silver Sulfadiazine Cream in Toxic Epidermal Necrolysis. Advances in Skin and Wound Care. 2014; 27(5):210–15. Crossref. PMid:24732124

Delay C, Cameron Journal of Wound Management. Oxfford: Wiley Blackwell; 2008. p.104.

Olczyk P, Mencner A, Vasse KK. The Role of the Extracellular Matrix Components in Cutaneous Wound Healing. Hindawi Publ Corp BioMed Res Int. 2014;1–7.


Refbacks

  • There are currently no refbacks.