Pimpinella alpina Molk Administration is Capable of Increasing Antioxidant and Decreasing Prooxidant Level following UVB Irradiation


  • Sultan Agung Islamic University, Department of Biochemistry, Faculty of Medicine, Semarang, Indonesia
  • Sultan Agung Islamic University, Department of Chemistry, Faculty of Medicine, Semarang, Indonesia


Introduction: Indonesian male population has traditionally used Pimpinella alpina Molk (PaM) to prevent degenerative disease. However, the scientific evidence of PaM effect on increase in antioxidant and decrease in prooxidant level and their negative correlation remains unclear. Objective: To prove the effect of PaM on increase in antioxidant and decrease in prooxidant and their negative correlation following Ultraviolet B (UVB) exposed repeatedly. Methods: Forty male rats were assigned into 8 groups, treatment groups for 7 days: PaM 50 mg (PaM50-7), PaM 100 mg (PaM100-7), PaM 150 mg (PaM150-7), and for 15 days: PaM 100 mg (PaM100-15), PaM 150 mg (PaM150-15). The increase in antoxidant and decrease in prooxidant levels were measured by ELISA and Spectophotometer. Results: Statistical analysis indicated that antioxidant Catalase (CAT) and Super Oxyde Dismutase (SOD) activities in PaM groups were significantly higher, p < 0.001. In contrary, prooxidant levels marked by Malondialdehide (MDA) and 8-hydroxy-2-deoxyguanosine (8OHdG) concentrations in PaM groups were significantly lower, p < 0.001. There was also a negative correlation between antioxidant and prooxidant levels, p < 0.001. Conclusion: PaM administration with 50-150 mg daily dosage for 7-15 days capable of increasing antioxidant and decreasing Prooxidant levels, with a negative correlation following UVB irradiation repeatedly.


Oxidative Stress, Pimpinella alpina Molk, CAT, MDA, SOD, 8OHdG

Full Text:


Abikusno N. Older population in Indonesia: Trends, issues and policy responses. Bangkok: UNFPA Indonesia and Country Technical Services Team for East and SouthEast Asia; 2007 Nov.

Noveria M. Challenges of population ageing in Indonesia. Conference on Impact of Ageing: A Common Challenge for Europe and Asia; Vienna. 2006 Jun 7-9.

McCarthy M. Boom in Latin American and Caribbean elderly population. The Lancet. 2004; 363:458–9. Crossref.

Dobriansky Pj, Suzman RM, Hodes RJ. Why population aging matters: A global perspective. National Institute on Aging National Institutes of Health. US Department of Health and Human Services. US Department of State; 2007.

Fries JF. Aging, natural death, and the compression of morbidity. The New England Journal of Medicine. 1980; 303:130–5. Crossref. PMid:7383070

Huebschmann AG, Kohrt WM, Regensteiner JG. Exercise attenuates the premature cardiovascular aging effects of type 2 diabetes mellitus. Vascular Medicine. 2011; 16(5):378–90. Crossref. PMid:21893560

Hillman CH, Kirk I. Erickson KI, Kramer AF. Be smart, exercise your heart: Exercise effects on brain and cognition. Neuroscience. 2008; 9:52–63. Crossref.

SyslovaK, Bohmova A, Mikoska M, Kuzma M, Pelclova D, KaIer P. Multimarker screening of oxidative stress in aging. Oxidative Medicine and Cellular Longevity. 2014; 1–14. Crossref. PMid:25147595 PMCid:PMC4124763

Taufiqurrachman. The effect of buceng extracts on androgen production in Sprague Dawley male rats. Medical Journal of Indonesia. 2012; 21(1):29–31. Crossref.

Afaq F, Syed DN, Malik A, Hadi N, Sarfaraz S. Delphinidin, an Anthocyanidin in Pigmented Fruits and Vegetables, Protects Human HaCaT Keratinocytes and Mouse Skin Against UVB Mediated Oxidative Stress and Apoptosis. Journal of Investigative Dermatology. 2007; 127:222–232. Crossref. PMid:16902416

Bowden GT. Prevention of non-melanoma skin cancer by targeting ultraviolet-B-light signalling. Nat Rev Cancer. 2004; 4:23–35. Crossref. PMid:14681688

Sproul CD, Mitchell DL, Rao S, Ibrahim JG, Kaufmann WK. Cyclobutane pyrimidine dimer density as a predictive biomarker of the biological effects of ultraviolet radiation in normal human fibroblast. Photochemistry and Photobiology. 2014; 90:145–54. Crossref. PMid:24148148 PMCid:PMC4454621

Chang H, Oehrl W, Elsner P, Thiele JJ. The role of H2O2 as a mediator of UVB-induced Apoptosis in Keratinocytes. Free Radical Research. 2003; 37:655–63. Crossref. PMid:12868492

Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. Oxidative stress and antioxidant defense. WAO Journal. 2012; 5:9–19. Crossref.

Cadet J, Sage E, Douki T. Ultraviolet radiation-mediated damage to cellular DNA. Mutat Res. 2005; 571:3–17. Crossref. PMid:15748634

Budiyanto A, Ahmed NU, Wu A, Bito T, Nikaido O, Osawa T et al. Protective effect of topically applied olive oil against photocarcino genesis following UVB exposure of mice. Carcinogenesis. 2000; 21:2085–90. Crossref. PMid:11062172

Clydesdale GJ, Dandie GW, Muller HK. Ultraviolet light induced injury: Immunological and inflammatory effects. Immunology and Cell Biology. 2001; 79:547–68. Crossref. PMid:11903614

De la Fuente M, Miquel J. An update of the oxidationinflammation theory of aging: The involvement of the immune system in oxi-inflamm-aging. Current Pharmaceutical Design. 2009; 15:3003–026. Crossref. PMid:19754376 19. Romano AD, Serviddio G, de Matthaeis A2, Bellanti F, Vendemiale G. Oxidative stress and aging. J Nephrol. 2010; 23(S15):S29–36. PMid:20872368

Suzery M, Cahyono B, Taufiqurrachman. Produksi Senyawa Afrodisiakdari Purwoceng (Pimpinella Alpina Molk): Pengembangan Potensi “Natural Resources” Khas Jawa Tengah. Diponegoro University Documentation; 2004.

Pandey AK, Mishra AK, Mishra A. Antifungal and antioxidative potential of oil and extracts derived from leaves of Indian spice plant Cinnamomumtamala. Cellular and Molecular Biology. 2012; 58:142–7. PMid:23273204

Rahal A, Kumar A, Singh V, Yadav B, Tiwari R, Chakraborty S, Dhama K. Oxidative stress, prooxidants, and antioxidants: The interplay. Bio Med Research International. 2014; 1–19. Crossref. PMid:24587990 PMCid:PMC3920909

Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: An overview. The Scientific World Journal. 2013; 1–16. Crossref. PMid:24327805 PMCid:PMC3845396

Chen WS, Lee YJ, Yu YC, et al. Enhancement of p53mutant human colorectal cancer cells radio sensitivity by flavonoid fisetin. Int J Radiation Oncology Biol Phys. 2010; 77:1527–35. Crossref. PMid:20637980

Cao G, Sofic E, Prior RL. Antioxidant and prooxidant behaviors of flavonoids: Structure activity relationships. Free Radic Biol Med. 1997; 22:749–60. Crossref.

Shadnia S, Azizi E, Hosseini R, Khoei S, Fouladdel S, Pajoumand A, Jalali N, Abdollahi M. Evaluation of oxidative stress and genotoxicity in organo phosphorus insecticide formulators. Human and Experimental Toxicology. 2005; 24:439–45. Crossref. PMid:16235732

Amaral S, Redmann K, Sanchez V, Mallidis C, Santos JR, Schlatt S. UVB irradiation as a tool to assess ROS-induced damage in human Spermatozoa. Andrology. 2013; 1: 707–14. Crossref. PMid:23836725

Erik L. Regalado, Rodríguez M, Menendez R, Angel A, et al. Repair of UVB-damaged skin by the antioxidant sulphated flavone glycoside thalassiolin B isolated from the marine plant thalassiatestudinum banks ex König. Mar Biotechnol. 2009; 11:74–80. Crossref. PMid:18607659

Hattori Y, Nishigori C, Tanaka T, Uchida K, Nikaido O, et al. 8-hydroxy-2’-deoxyguanosme is increased in epidermal cells of hairless mice after chronic ultraviolet B exposure. J Invest Dermatol. 1997; 107:733–7. Crossref.

Halliwell B, Aruoma OI. DNA and free radicals. Chichester, UK: Ellis Horwood; 1993.

Clarkson PM and Thompson HS. Antioxidants: what role do they play in physical activity and health? Am J ClinNutr. 2000; 72(suppl):637S–46S. Crossref. PMid:10919970

Santos AL, Oliveira V, Baptista I, Henriques I, Gomes NCM, et al. (2013) Wavelength dependence of biological damage induced by UV radiation on bacteria. Arch Microbiol 2013; 195:63–74. Crossref. PMid:23090570

Zhan JYX, Wang XF, Liu YH, Zhang ZB, Wang L et al. Andrographolide sodium bisulfate prevents UV-induced skin photoaging through inhibiting oxidative stress and inflammation. Mediators of Inflammation. 2016; 1–12. Crossref. PMid:26903706 PMCid:PMC4745921

Cejkova J, Stipek S, Crkovska J, Ardan T. Changes of superoxide dismutase, catalase and glutathione peroxidase in the corneal epithelium after UVB rays. Histochemical and biochemical study. Histol Histopathol. 2000; 15:1043–50. PMid:11005228

Yin Y, Li W, Son YO, Sun L, Lu J, et al. Quercitrin protects skin from UVB-induced oxidative damage. Toxicol Appl Pharmacol. 2013; 269(2):89–9. Crossref. PMid:23545178 PMCid:PMC3700335

Dalton TP, Shertzer HG, Puga A. Regulation of gene expression by reactive oxygen. Ann Rev Pharmacol Toxicol. 1999; 39:67–101. Crossref. PMid:10331077 37. Okada K. Takahashi Y. Ohnishi K. Isliikawa O, Miyachi Y. Time dependent effect of chronic UV irradiation on superoxide dismutase and catalase activity in hairless mice skin. J Deiinalol Sci. 1994; 8:183–6. Crossref.

Gul MZ, Ahmad F, Kondapi AK, Qureshi IA, Ghazi IA. Antioxidant and antiproliferative activities of Abrusprecatorius leaf extracts - an in vitro study. BMC Complementary and Alternative Medicine. 2013; 13:53. Crossref. PMid:23452983 PMCid:PMC3600005

Fukushima K, Murata M, Tsukimori K, Eisuke K, Wake N. 8-hydroxy-2-deoxyguanosine staining in placenta is associated with maternal serum uric acid levels and gestational age at diagnosis in pre-eclampsia. American Journal of Hypertension. 2011; 24(7):829–33. Crossref. PMid:21415844

Zern TL, Fernandez ML. Cardioprotective effects of dietary polyphenols. J Nutr. 2005; 135:2291–4. Crossref. PMid:16177184

Giovannini C, Masella R. Role of polyphenols in cell death control. Nutritional Neuroscience. 2012; 15(3):134–9. Crossref. PMid:22584012

Van A, van den Berg SABE, Tromp DJMNJL et al. Structural aspects of antioxidant activity of flavonoids. Free Radical Biology and Medicine. 1996; 20(3):331–42. Crossref.

Pannala AS, Chan TS, Brien PJO, Rice Evans CA. Flavonoid B-ring chemistry and antioxidant activity: Fast reaction kinetics. Biochemical and Biophysical Research Communications. 2001; 282(5):1161–8. Crossref. PMid:11302737

Kelly EH, R. Anthony T, Dennis JB. Flavonoid antioxidants: Chemistry, metabolism and structureactivity relationships. Journal of Nutritional Biochemistry. 2002; 13(10):572–84. Crossref.

Bakir T, Sonmezoglu I, Imer F, Apak RS. Antioxidant/ prooxidant effects of a-tocopherol, quercetin and isorhamnetin on linoleic acid peroxidation induced by Cu(II) and H2O2. Int J Food Sci Nutr. 2014; 65(2):226–34. Crossref. PMid:24152374

Blessing EO, Francis M. Dose-related effect of quercetin in the human breast carcinoma MCF-7 cell line. American Journal of Scientific and industrial research. Science. 2010. Available from: Crossref.

Resende FA, Vilegas W, Santos LC, Varanda EA. Mutagenicity of flavonoids assayed by bacterial reverse (Ames) test. Molecules. 2012; 17:5255–68. Crossref. PMid:22565478

Sghaier MB, Skandrani I, Nasr N, Franca MGD, Ghedira LC, Ghedira K. Flavonoids and sesquiterpenes from Tecuriumramosissimum promote antiproliferation of human cancer cells andenhance antioxidant activity: A structure–activity relationship study. Environmental Toxicology and Pharmacology. 2011; 32:336–48. Crossref. PMid:22004952

Farombi EO, Abarikwu SO, Adesiyan AC, Oyejola TO. Quercetin exacerbates the effects of subacute treatment of atrazine on reproductive tissue antioxidant defence system, lipid peroxidation and sperm quality in rats. Andrologia. 2013; 45:256–65. Crossref. PMid:22897171

Yin Y, Li W, Son YO, Sun L, Lu J, et al. Quercitrin protects skin from UVB-induced oxidative damage. Toxicol Appl Pharmacol. 2013; 269(2):89–99. Crossref. PMid:23545178 PMCid:PMC3700335


  • There are currently no refbacks.