Pimpinella alpina Molk Administration is Capable of Increasing Antioxidant and Decreasing Prooxidant Level following UVB Irradiation

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Authors

  • Department of Biochemistry, Faculty of Medicine, Sultan Agung Islamic University, Semarang ,ID
  • Department of Chemistry, Faculty of Medicine, Sultan Agung Islamic University, Semarang ,ID

DOI:

https://doi.org/10.18311/jnr/2018/20187

Keywords:

Oxidative Stress, Pimpinella alpina Molk, CAT, MDA, SOD, 8OHdG

Abstract

Introduction: Indonesian male population has traditionally used Pimpinella alpina Molk (PaM) to prevent degenerative disease. However, the scientific evidence of PaM effect on increase in antioxidant and decrease in prooxidant level and their negative correlation remains unclear. Objective: To prove the effect of PaM on increase in antioxidant and decrease in prooxidant and their negative correlation following Ultraviolet B (UVB) exposed repeatedly. Methods: Forty male rats were assigned into 8 groups, treatment groups for 7 days: PaM 50 mg (PaM50-7), PaM 100 mg (PaM100-7), PaM 150 mg (PaM150-7), and for 15 days: PaM 100 mg (PaM100-15), PaM 150 mg (PaM150-15). The increase in antoxidant and decrease in prooxidant levels were measured by ELISA and Spectophotometer. Results: Statistical analysis indicated that antioxidant Catalase (CAT) and Super Oxyde Dismutase (SOD) activities in PaM groups were significantly higher, p < 0.001. In contrary, prooxidant levels marked by Malondialdehide (MDA) and 8-hydroxy-2-deoxyguanosine (8OHdG) concentrations in PaM groups were significantly lower, p < 0.001. There was also a negative correlation between antioxidant and prooxidant levels, p < 0.001. Conclusion: PaM administration with 50-150 mg daily dosage for 7-15 days capable of increasing antioxidant and decreasing Prooxidant levels, with a negative correlation following UVB irradiation repeatedly.

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Published

2018-05-09

How to Cite

Nasihun, T., & Widayati, E. (2018). <I>Pimpinella alpina</I> Molk Administration is Capable of Increasing Antioxidant and Decreasing Prooxidant Level following UVB Irradiation. Journal of Natural Remedies, 18(1), 29–39. https://doi.org/10.18311/jnr/2018/20187

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Articles
Received 2018-03-20
Accepted 2018-04-19
Published 2018-05-09

 

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