Ameliorative Effects of Operculina turpethum and its Isolated Stigma‑5,22dien‑3-o-b-D-glucopyranoside on the Hematological Parameters of Male Mice Exposed to N‑Nitrosodimethylamine, a Potent Carcinogen


Affiliations

  • Banasthali University, Department of Bioscience and Biotechnology, Banasthali, Rajasthan, India

Abstract

Objectives: Enormous propensity of plants to synthesize a variety of structurally diverse bioactive compounds, has made the plant kingdom a potential source of chemical constituents with various therapeutic values, including antitumor and cytotoxic activities. Blood is a good indicator to determine the physiological and pathological status of man and animal. The objective of the present study is to determine the effect of Operculina turpethum root extract and its isolated glycoside treatment on the hematological parameters in the mice with N‑Nitrosodimethylamine (NDMA) induced cancer. Materials and Methods: The body weights of the animals were recorded before and after the experiment. Non‑coagulated blood was tested for total erythrocyte count, total leukocyte count, hemoglobin, differential leukocyte count (DLC) and for other blood indices. Results: A significant (P < 0.01), (P < 0.001) recovery of the red blood cell and white blood cell counts, packed cell volume and hemoglobin content in the host after 21 day treatment was shown. Conclusion: These results show that the extract of Operculina turpethum is relatively safe following oral administration and have possible stimulatory effect on red blood cell production and there was dose dependent therapeutic effect.

Keywords

Blood, haematology, N‑Nitrosodimethylamine, Operculina turpethum

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