Role of Ocimum sanctum as a Genoprotective Agent on Chlorpyrifos-Induced Genotoxicity


Affiliations

  • St. Aloysius’ College, Department of Zoology and Biotechnology, India
  • Government Science College, Pachpedi, Jabalpur, MP, India
  • Mata Gujri Women’s College, Jabalpur, Madhya Pradesh, India

Abstract

Protective effect of Ocimum sanctum was evaluated on chlorpyrifos-induced genotoxicity in in vivo and in vitro models. Two different concentrations of pesticide were taken, i.e., 1/5 and 1/15 of LD50 of chlorpyrifos for the in vivo study. Rats were pre-treated orally with O. sanctum extract (OE) at 50 mg/kg b.wt. For thein vitro studies, human lymphocyte cultures were exposed to 75 μg/ml chlorpyrifos with and without OE. Structural and numerical (both aneuploidy and euploidy types) chromosomal aberrations (CAs) were scored for the assessment of induced genotoxic effects, while the variation in mitotic index (MI) was considered as a monitor for induced cellular toxicity. The same concentration of the pesticide (75 μg/ml) was taken to study the DNA damage by comet assay. Results showed that lymphocytes treated with the pesticide exhibited increased DNA damage but the increase was statistically insignificant (P>0.05). In rats pretreated with OE, a significant (P<0.01) increase in MI was observed and there was a significant decrease in the frequency of aberrant cells as compared to the rats treated with chlorpyrifos alone. A significant (P<0.05) increase in CA was observed in cultures treated with 75 μg/ml chlorpyrifos as compared to controls, which decreased significantly (P<0.05) with OE pretreatment.

Keywords

Chlorpyrifos, chromosomal aberration, comet assay, Ocimum sanctum

Subject Discipline

Zoology

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References

WHO specifications and evaluations for public health pesticides, Evaluation Report 221, Chlorpyrifos. Geneva: WHO; 2002. p. 13.

Satyawati GV, Gupta KA, Tandon N. Ocimum sanctum (Linn.) in Medicinal Plants of India. New Delhi: Indian Council for Medical Research; 1987.

Aruna K, Shivaramakrishnan VM. Plant products as protective agents against cancer. Indian J Exp Biol 1990;28:1008-11.

Uma Devi P. Radio protective, anticarcinogenic and antioxidant properties of the Indian holy basil, Ocimum sanctum (Tulsi). Indian J Exp Biol 2001;39:185-90.

Uma Devi P, Ganasoundari A, Vrinda B, Srinivasan KK, Unnikrishnan MK. Radiation protection by the Ocimum flavonoids orientin and vicenin: Mechanism of action. Radiat Res 2000;154:455-60.

Balaji M, Sasikala K. Cytogenetic effects of malathion in in vitro culture of human peripheral blood. Mutat Res 1993;301:13-7.

Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988;175:184-91.

Nadin SB, Vargas-Roig LM, Ciocca DZ. A silver staining method for single- cell Gel Assay. J Histochem Cytochem 2001;49:1183-6.

Silva J, Freitas TR, Marinho JR, Speit G, Erdtmann B. An alkaline single-cell gel electrophoresis (comet) assay for environmental biomonitoring with native rodents. Genetics Mol Biol 2000;23:241-5.

Uma Devi P, Ganasoundari A. Radioprotective effect of leaf extract of Indian medicinal plant Ocimum sanctum. Indian J Exp Biol 1995;33:205-8.

Moorhead PS, Nowell PC, Mellman WJ, Battips DM, Hungerford DA. Chromosome preparations of Leucocytes Cultured from Human Peripheral Blood. Exp Cell Res 1960;20:613-6.

Rahman MF, Mahboob M, Danadevi K, Saleha Banu B, Grover P.

Assessment of genotoxic effects of chlorpyrifos and acephate by comet assay in mice leukocytes. Mutat Res 2002;516:139-47.

Ribas G, Frenzilli G, Barale R, Marcos R. Herbicide-induced DNA damage in human lymphocytes evaluated by the single-cell gel electrophoresis (SCGE) assay, Mutat Res 1995;344:41-54.

Mehta A, Verma RS, Srivastava N. Chlorpyrifos induced DNA damage in rat liver and brain cells. Environ Mol Mutagen 2008;49:426-33.

Vigreux C, Poul JM, Deslandes E, Lebailly P, Godard T, Sichel F, et al. DNA damaging effects of pesticides measured by the single cell gel electrophoresis assay (comet assay) and the chromosomal aberration test, in CHOK1 cells. Mutat Res 1998;419:79-90.

Paramjit G, Danadevi K, Mahboob M, Rozati R, Banu BS, Rahman MF. Evaluation of genetic damage in workers employed in pesticide production utilizing the comet assay. Mutagenesis 2003;18:201-5.

Bolognesi C. Genotoxicity of pesticides: A review of human biomonitoring studies. Mutat Res 2003;543:251-72.

Awa AA.Chromosome damage in atomic bomb survivors and their offspring- Hiroshima and Nagasaki. In Radiation-induced cytogenetic damage in Man. T. Ishihara and M.S. Sasaki, ed. New York: Alan R. Liss; 1983;433-453

Au WW, Cantelli-Forti G, Hrelia, P, Legator MS. Cytogenetic assays in genotoxic studies, somatic cell effects of benzene and germinal cell effects of dibromochloropropane. Teratog Carcinog Mutagen 1990;10:125-34.

Von Sontag C. The chemical basis for Radiation Biology. London: Taylor and Francis; 1987.

Bagchi D, Bagchi M, Hassoun EA, Stohs SS. In vitro and in vivo generation of reactive oxygen species, DNA damage and LDH leakage by selected pesticides. Toxicol 1999;104:129-30.


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