Variability in parasitic ability of Trichoderma isolates against sclerotia of Sclerotium rolfsii associated with chickpea


  • Regional Agricultural Research Station, Tirupati - 517 502, Andhra Pradesh
  • NRRI-Central Rainfed Upland Rice Research Station, Hazaribagh, Jarkhand
  • Department of Plant Protection, Palli siksha Bavana, Viswava Bharati, Bolpur – 731235, West Bengal
  • Regional Agricultural Research Station, Tirupati - 517 502, Andhra Pradesh



Chickpea, Competitive parasitic ability, Trichoderma spp


Parasitic ability of Trichoderma isolates was tested against Sclerotium rolfsii in two types of soils viz., natural and sterilized soils to assess the ecological adaptability of Trichoderma spp. to a soil. The isolate ATPU 1 (EID50 of 2.1 mycelia/gm of soil) was most effective in parasitization of sclerotia of S. rolfsii when used as live bait, under the natural soil of, when mycelial form of inoculum was used. This isolate was closely followed by KNO 2 and ATPP 6 which had the same EID50 of 2.2 mycelia/gm of soil, the next best isolates were KNP 3 and ATPPE 6 with EID50 of 2.3 mycelia/gm of soil. The isolate KT 6 (EID50 5.7) was rated as poorest competitive colonizer, requiring comparatively highest inoculum level to colonize 50% sclerotia of S. rolfsii . However the conidial form of inoculum from KNK1 (EID50 value of 2.2 conidia/gm of soil) was most aggressive isolate requiring lowest inoculum dose for 50% colonization of sclerotia of S. rolfsii.


Download data is not yet available.


Metrics Loading ...

Author Biography

P. Nagamani, Regional Agricultural Research Station, Tirupati - 517 502, Andhra Pradesh

Department of Plant Pathology


Bae YS, Knudsen GR. 2005. Soil microbial biomass on growth and biocontrol efficacy of Trichoderma harzianum. Biological Contro. 32: 236–242. Crossref.

Benhamou N, Chet I. 1996. Parasitism of scletotia of S. rolfsii by Trichoderma harzianum: Ultrastructural and Cytochemical aspects of the interaction. Phytopathology 86: 405–416.Crossref.

Bhagat S, Pan S. 2011. Parasitic ability of Trichoderma isolates against sclerotia of Sclerotium rolfsii and management of collar rot of brinjal. Biopesticide Int. 7(1): 52–59.

Bhagat S, Birah A, Kumar R, Yadav MS, Chattopadhyay C. 2014. Plant disease management: prospects of pesticides of plant origin. Advances in Plant Biopesticides 119–129.

Bhagat S, Pan S. 2011. Competitive parasitic ability of Trichoderma spp. and biocontrol of damping off of solanaceous vegetables. J Mycopathol Res. 49(1): 65–72.

Bunker RN, Mathur K. 2001. Antagonism of local biocontrol agents to Rhizoctonia solani inciting dry root rot of chilli. Indian J Mycol Pl Pathol. 31: 50–53.

Cole-Smith JR, Cook RC. 1971. Survival and germination of fungal sclerotia Ann Rev Phytopathol. 9: 65–92. Crossref.

Cook RC, Al-Hamdani AM. 1986. Water relations of sclerotia and other infective structure. In: Ayes PG, Body L (Eds.). Water, Fungi and Plants. Cambridge: Canbridge University Press. 49–63. PMid:3957782

Harman GE. 1991. Seed treatments for biological control of plant disease. Crop Prot. 10: 166–171. Crossref.

Monika S. Sharma OP, Bhagat S, Neetu P. 2016. In-Vivo Evaluation of Competitive parasitic Ability and Rhizosphere Colonisation of Different Trichoderma isolates. Int J Curr Microbiol Appl Sci. 5 (4): 32–38. Crossref.

Mukherjee PK, Mukhopadhyay AN, Sarmah DK, Shrestha SM. 1995. Comparative Antagonistic Properties of Gliodadium virens and Trichoderma harzianum on Sclerotium rolfsii and Rhizoctonia solani ”its Relevance to Understanding the Mechanisms of Biocontrol. J Phytopathol. 143: 275–279. Crossref.

Muthamilan M, Jeyarajan R. 1992. Effect of antagonistic fungi on Sclerotium rolfsii causing root rot of groundnut. J Biol Control 6: 88–92.

Pan S, Bhagat S. 2008. Characterization of antagonistic potential of Trichoderma spp. against some soil borne plant pathogens. J Bioi Control 22(1): 43–49

Roy A, Pan S. 2005. Competitive parasitic ability and biocontrol potential of mutants of Trichoderma harzianum and Gliocladium virens against Sclerotium rolfsii induced root rot of groundnut. Indian J Mycol Pl Pathol. 35 326–330.

Smith VL, Wilcox WF, Harman GE. 1990. Biological potential for biological control of phytophthora root rot and crown rots of apple by Trichoderma and Gliocladium species. Phytopathology 80: 880– 885. Crossref.

Vincent JH. 1947. Distortion of fungal hyphae in the presence of certain inhibitors. Nature 15: 850. Crossref.




How to Cite

Nagamani, P., Bhagat, S., Biswas, M. K., & Viswanath, K. (2018). Variability in parasitic ability of <i>Trichoderma</i> isolates against sclerotia of <i>Sclerotium rolfsii</i> associated with chickpea. Journal of Biological Control, 31(3), 181–188.



Research Articles