In vitro and In planta Assays for Biological Control of Fusarium Root Rot Disease of Vanilla
Keywords:Biological Control, Colletotrichum Sp., Fusarium oxysporum F. Sp. Vanillae, In vitro Screening, Root Rot and Vanilla planifolia.
AbstractRhizosphere and phyllosphere organisms isolated from vanilla consisted of predominant colonizers such as Fusarium sp. (24 isolates) and Colletotrichum sp. (20 isolates). Other organisms were Rhizoctonia sp. (two isolates), Trichoderma spp. (seven isolates), Paecilomyces sp. (one isolate), Mucor sp. (three isolates), non-sporulating fungal species (10 isolates) and Pseudomonas fluorescens (three isolates). When tested in vitro, six isolates showed more than 50% inhibition of Fusarium oxysporum f. sp. vanillae. These were two isolates of Trichoderma sp. (53.30-70.58), one isolate of Paecilomyces sp. (65.00%) and two isolates of P. fluorescens (59.15- 62.50%) that were antagonistic to the pathogen. None of the non-pathogenic Fusarium species tested showed promising inhibitory effect on F. oxysporum f. sp. vanillae. The five promising isolates were tested in planta by challenge inoculation. Paecilomyces sp. provided 100 per cent protection against root rot. T. harzianum and P. fluorescens provided 40% protection. Thus the present study indicated the possibility of using Paecilomyces sp. as a potential antagonist for F. oxysporum f. sp. vanillae.
Bhai, R. S. and Thomas, J. 2000. Phytophthora rot, a new disease of vanilla (Vanilla planifolia Andrews) in India. Journal of Spices and Aromatic Crops, 9: 73-75.
Bhai, R. S., Bhat, A. I and Anandaraj, M. 2006. Yellowing and premature bean dropping in vanilla (Vanilla planifolia Andrews). Journal of Plantation Crops, 34: 115-117.
Deqvaire, J. 1976. Improvement of vanilla plant at Madagascar. J D'Agric Tropicale et de Botanique Appliquée 23: 139-158.
Khan, T. A., Khan, S.T., Fazal. M and Siddique, Z. A. 1997. Biological control of Meloidogyne incognita and Fusarium solani disease complex in papaya using Paecilomyces lilacinus and Trichoderma harzianum. International Journal of Nematology, 7:127-132.
Fang, L., Huai, S., Bo, L. and Hua, C. J. 2005. Antagonistic effect of biocontrol fungus, Paecilomyces lilacinus strain NH-PL-03 and its mechanism against Fusarium oxysporum. Acta Phytophylactica Sinica, 32 : 373-378.
Fang, L., Bin, G. C., Bo, L. and Hua, C. J. 2006. The inhibition effect of secondary metabolite of Paecilomyces lilacinus against Fusarium oxysporum. Acta Phytophylactica Sinica, 33: 94- 98.
Praveen, S. and Ghaffar, A. 1998. Use of Paecilomyces lilacinus in the control of Fusarium oxysporum root rot and Meloidogyne javanica root knot infection on tomato. Pakistan Journal of Nematology, 16: 71-75.
Purseglove, J. W., Brown, E. G., Green, C. L. and Robbins, S. R J. 1988. Spices Vol.2. Tropical Agriculture Series. Longman Scientific and Technical, England, 813p.
Shahnaz Dawar, and Ghaffar, A. 2003. Effect of inorganic fertilizers on the efficacy of Paecilomyces lilacinus in the control of soil borne root infecting fungi on mung bean. Pakistan Journal of Botany, 35: 479-482.
Sosamma, V. K. and Koshy, P. K. 1997. Biological control of Meloidogyne incognita on black pepper by Pasteuria penetrans and Paecilomyces lilacinus. Journal of Plantation Crops, 25: 72-76.
Tombe, M., Tsuchiya, K., Nurawan, A., Nazurudin, S. B., Oniki, M. and Matsumoto, K. 1992. Experiments on the introduction of biological and cultural control of stem rot disease of vanilla. Industrial Crop Research Journal, 4: 20-26.
Tombe, M, Kobayashi, K. and Ogoshi, A. 1994. Vegetative compatibility grouping of Fusarium oxysporum f. sp. vanillae in Indonesia. Indonesia Journal of Crop Science, 9: 29-39.