Development of Species Specific DNA Marker as Barcode Sequence of Greater Indian Rhinoceros [Rhinoceros unicornis] from Northeast India

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Authors

  • Sankar Kumar Ghosh
     Department of Biotechnology, Assam University, Silchar-788011, Assam ,IN
  • Prabal Ranjan Ghosh
     Department of Vetarinary Physiology, WBUAFS, Kolkata-700 037, W.B. ,IN
  • Tarikul Huda Mazumder
     Department of Biotechnology, Assam University, Silchar-788011, Assam ,IN
  • Miranda Kshetrimayum
     Department of Biotechnology, Assam University, Silchar-788011, Assam ,IN

Keywords:

DNA Barcoding, Mitochondrial DNA, COI-5', Rhinoceros Unicornis.

Abstract

Northeast India is a reservoir of rich biodiversity, especially Greater one-horned rhinoceros (Rhinoceros unicornis) which is facing extinction. DNA barcoding being an exciting tool for accurate documention of biodiversity with a gene sequence, in the present article DNA barcode of R. unicornis is determined for the first time by amplifying mitochondrial COI gene region using cross species primer designing and other bioinformatics analysis (Accession No. JN41700). Phylogeny analysis of obtained sequence with other related species distinctly maintains the assigned taxonomic position.

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Issue

Volume 10,Issue 1,2013

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Articles

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