Stomatal Analysis of Allium wallichii Kunth Leaves: An Experimental Finding through Quantitative Microscopy

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Authors

  • Faculty of Pharmacy, DIT University, Mussoorie Diversion Road, Dehradun, Uttarakhand – 248009 ,IN
  • Department of Pharmaceutical Sciences, Sir J. C. Bose Technical Campus Bhimtal, Kumaun University Nainital, Uttarakhand – 263136 ,IN
  • Institute of Bioresources and Sustainable Development, Imphal, Manipur – 795001 ,IN
  • Amity Institute of Pharmacy, Amity University, Gurugram, Haryana – 121004 ,IN
  • Faculty of Pharmacy, DIT University, Mussoorie Diversion Road, Dehradun, Uttarakhand – 248009 ,IN

DOI:

https://doi.org/10.18311/jnr/2022/30206

Keywords:

Allium wallichii, Microscopy, Stomata, Stomatal Index, Quality Control

Abstract

The use of microscopic techniques for the identification of medicinal plants is one of the best practices and an important quality control tool for standardization. Stomata are important organs for the study of phylogenetic relationships including plant origin, evolution, and classification. Allium wallichii (A. wallichii) Kunth, is one of the threatened medicinal herbs having important nutraceutical, medicinal and therapeutic value. The plant is one of the neglected species of Allium and has not been thoroughly investigated pharmacognostically. Therefore, current research work was designed to investigate the stomata types, stomata number and stomatal index through quantitative microscopy. The result presented in the current research will provide a routine identification method for A. wallichii with the allied species of Allium.

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Published

2022-09-27

How to Cite

Rana, V. S., Bisht, D., Chaudhary, S. K., Gupta, R., & Sethiya, N. K. (2022). Stomatal Analysis of <i>Allium wallichii</i> Kunth Leaves: An Experimental Finding through Quantitative Microscopy. Journal of Natural Remedies, 22(4), 691–696. https://doi.org/10.18311/jnr/2022/30206

Issue

Section

Short Communication
Received 2022-05-08
Accepted 2022-07-12
Published 2022-09-27

 

References

Melotto M, Underwood W, He SY. Role of stomata in plant innate immunity and foliar bacterial diseases. Annu Rev Phytopathol. 2008; 46:101-122. https://doi.org/10.1146/annurev.phyto.121107.104959 DOI: https://doi.org/10.1146/annurev.phyto.121107.104959

Alam F, Saqib QN. Pharmacognostic standardization and preliminary phytochemical studies of Gaultheria trichophylla. Pharm Biol. 2015; 53(12):1711-1718. https://doi.org /10.3109/13880209.2014.1003355 DOI: https://doi.org/10.3109/13880209.2014.1003355

Sethiya NK, Trivedi A, Patel MB, Mishra SH. Comparative pharmacognostical investigation on four ethanobotanicals traditionally used as Shankhpushpi in India. J Adv Pharm Technol Res. 2010; 1(4):388-395. https://doi.org/10.4103/0110-5558.76437 DOI: https://doi.org/10.4103/0110-5558.76437

Sethiya NK, Brahmbhat K, Chauhan B, Mishra SH. Pharmacognostic and phytochemical investigation of Ensete superbum (Roxb.) Cheesman pseudostem. Indian J Nat Pro Resour. 2016; 7(1):51-58.

Trivedi A, Sethiya NK, Mishra SH. Preliminary pharmacognostic and phytochemical analysis of “Granthika” (Leonotis nepetaefolia): An Ayurvedic herb. Indian J. Tradit. Knowl. 2011; 10(4):682-688.

Bhatt DY, Sethiya NK, Mishra SH. Pharmacognostic studies on the aerial parts of Suaeda maritima Linn. (Chenopodiaceae). Niger J Nat Prod Med. 2010; 14:1-5. https://doi.org/10.4314/njnpm.v14i1.1 DOI: https://doi.org/10.4314/njnpm.v14i1.1

Tiwari U, Adams SJ, Begum N, Krishnamurthy KV, Ravikumar K, Padma V. Pharmacognostic studies on two Himalayan species of traditional medicinal value: Allium wallichii and Allium stracheyi. Not Sci Biol. 2014; 6(2):149- 154. https://doi.org/10.15835/nsb629308 DOI: https://doi.org/10.15835/nsb629308

Chhetri HB, Gupta VNP. A survey of non-timber forest products (NTFPS) in upper Mustang. Sci World. 2007; 5(5):89-94. https://doi.org/10.3126/sw.v5i5.2663 DOI: https://doi.org/10.3126/sw.v5i5.2663

Rana MS, Samant SS. Threat categorisation and conservation prioritisation of floristic diversity in the Indian Himalayan region: A state of art approach from Manali Wildlife Sanctuary. J Nat Conserv. 2010; 18:159-168. https://doi.org/10.1016/j.jnc.2009.08.004 DOI: https://doi.org/10.1016/j.jnc.2009.08.004

Huang D, Li Q, Zhou C, Zhou S, He X. Intraspecific differentiation of Allium wallichii (Amaryllidaceae) inferred from chloroplast DNA and internal transcribed spacer fragments. J Syst Evol. 2014; 52:341-354. https://doi.org/10.1111/jse.12050 DOI: https://doi.org/10.1111/jse.12050

Chen X, Krug L, Yang M, Berg G, Cernava T. The Himalayan onion (Allium wallichii Kunth) harbors unique spatially organized bacterial communities. Microb Ecol. 2021; 82:909-918. https://doi.org/10.1007/s00248-021-01728-5 DOI: https://doi.org/10.1007/s00248-021-01728-5

Sundriyal M, Sundriyal RC, Sharma E. Dietary use of wild plant resources in the Sikkim Himalaya, India. Econ Bot. 2004; 58(4):626-638. https://doi.org/10.1663/0013- 0001(2004)058[0626:DUOWPR]2.0.CO;2 DOI: https://doi.org/10.1663/0013-0001(2004)058[0626:DUOWPR]2.0.CO;2

Singh G, Rawat MS, Pandey D, Rawat GS. Status and traditional uses of medicinal plants in Mandal area of Western Himalaya, India. Med Plants - Int J Phytomed Relat Ind. 2011; 3(2):95-104. https://doi.org/10.5958/j.0975-4261.3.2.016 DOI: https://doi.org/10.5958/j.0975-4261.3.2.016

Rai U, Rai B. Plant based raw drugs sold in the markets of the Darjeeling Himalaya, India: A case study. Pleione. 2020; 14(2):293-299. https://doi.org/10.26679/ Pleione.14.2.2020.293-299 DOI: https://doi.org/10.26679/Pleione.14.2.2020.293-299

Kunwar RM, Nepal BK, Kshhetri HB, Rai SK, Bussmann RW. Ethnomedicine in Himalaya: A case study from Dolpa, Humla, Jumla and Mustang districts of Nepal. J Ethnobiol Ethnomed. 2006; 2:27. https://doi.org/10.1186/1746-4269- 2-27 DOI: https://doi.org/10.1186/1746-4269-2-27

Topwal M, Uniyal S. Review on important ethno-medicinal plants in Uttarakhand. Int J Pure App Biosci. 2018; 6(2):455- 464. https://doi.org/10.18782/2320-7051.6398 DOI: https://doi.org/10.18782/2320-7051.6398

Khakurel D, Yadav U, Luczaj L, Rajbhandary S. Foods from the wild: Local knowledge, use pattern and distribution in Western Nepal. PLoS One. 2021; 16(10):e0258905. https://doi.org/10.1371/journal.pone.0258905 DOI: https://doi.org/10.1371/journal.pone.0258905

Semwal DP, Bhatt KC, Mamgain A, Uniyal PL, Bahuguna YM. Role of potential ethno-medicinal plants resources of Kalimath valley (District: Rudraprayag) Uttarakhand, India in health care. Med Plants - Int J Phytomed Relat Ind. 2013; 5(4):223-230. https://doi.org/10.5958/j.0975-6892.5.4.036 DOI: https://doi.org/10.5958/j.0975-6892.5.4.036

Semwal DP, Kala CP, Bhatt AB. Medicinal Plants and Traditional Health Care Knowledge of Vaidyas, Palsi and Others: A Case Study from Kedarnath Valley of Uttarakhand, India. Medicinal Plants. 2010; 2(1):51-57. https://doi.org/10.5958/j.0975-4261.2.1.007 DOI: https://doi.org/10.5958/j.0975-4261.2.1.007

Bhattarai S, Chaudhary RP, Quave CL, Taylor RSL. The use of medicinal plants in the trans-himalayan arid zone of Mustang district, Nepal. J Ethnobiology Ethnomedicine. 2010; 6:14. https://doi.org/10.1186/1746-4269-6-14 DOI: https://doi.org/10.1186/1746-4269-6-14

Ong HG, Ling SM, Win TTM, Kang D, Lee J, Kim Y. Ethnomedicinal plants and traditional knowledge among three Chin indigenous groups in Natma Taung National Park (Myanmar). J Ethnopharmacol. 2018; 225:136-158. https://doi.org/10.1016/j.jep.2018.07.006 DOI: https://doi.org/10.1016/j.jep.2018.07.006

Singh A, Hart R, Chandra S, Nautiyal MC, Sayok AK. Traditional herbal knowledge among the inhabitants: a case study in Urgam Valley of Chamoli Garhwal, Uttarakhand, India. Evid-based Complement Altern Med. 2019; 5656925:1-21. https://doi.org/10.1155/2019/5656925 DOI: https://doi.org/10.1155/2019/5656925

Miya MS, Timilsina S, Chhetri A. Ethnomedicinal uses of plants by major ethnic groups of Hilly Districts in Nepal: A review. J Med Bot. 2020; 4:24-37. https://doi.org/10.25081/jmb.2020.v4.6389 DOI: https://doi.org/10.25081/jmb.2020.v4.6389

Adhikari K, Uprety Y, Pyakurel D, Thagunna K, Bhatta LD, Chaudhary RP. Healthcare choices and use of medicinal plants: An ethnobotanical study in Kanda Area of Bajhang District, Kailash Sacred Landscape, Nepal. In: Abbasi, A.M., Bussmann, R.W. (eds) Ethnobiology of Mountain Communities in Asia. Ethnobiology. Springer, Cham. 2021. https://doi.org/10.1007/978-3-030-55494-1_18 DOI: https://doi.org/10.1007/978-3-030-55494-1_18

Bhandari P, Gurung MB, Subedi CK, Chaudhary RP, Basnet K, Gurung J, Yadav U, Neupane A, Shrestha KK. Traditional use of medicinal plants in the Chyangthapu-Phalaicha biological sub-corridor, Panchthar District, Kangchenjunga Landscape, Nepal. Ethnobot Res Appl. 2021; 22:25. https://doi.org/10.32859/era.22.25.1-43 DOI: https://doi.org/10.32859/era.22.25.1-43

Khajuria AK, Manhas RK, Kumar H, Bisht NS. Ethnobotanical study of traditionally used medicinal plants of Pauri district of Uttarakhand, India. J Ethnopharmacol. 2021; 276:114204. https://doi.org/10.1016/j.jep.2021.114204 DOI: https://doi.org/10.1016/j.jep.2021.114204

Sornes E, Risal A, Manandhar K, Thomas H, Steiner TJ, Linde M. Use of medicinal plants for headache, and their potential implication in medication-overuse headache: Evidence from a population-based study in Nepal. Cephalalgia. 2021; 41(5):561-581. https://doi.org/10.1177/0333102420970904 DOI: https://doi.org/10.1177/0333102420970904

Bhandari J, Muhammad BT, Thapa P, Shrestha BG. Study of phytochemical, anti-microbial, anti-oxidant, and anticancer properties of Allium wallichii. BMC Complement Altern Med. 2017; 17:102. https://doi.org/10.1186/s12906-017-1622-6 DOI: https://doi.org/10.1186/s12906-017-1622-6

Kamal R, Sharma GL. Steroidal sapogenins from bulbs of Allium wallichii Kunth. Pharmazie. 1984; 39(10):707-708.

Kattel A, Maga JA. Volatile compounds from dried Jimbu (Allium wallichii). Dev Food Sci. 1995; 37:919-928. https://doi.org/10.1016/S0167-4501(06)80207-4 DOI: https://doi.org/10.1016/S0167-4501(06)80207-4

Wang H, Yang Q, Li F, Song J, Zhang X, Li X. Investigation and Analysis of Nutrition Components of Wild Chives Collected from Hezhang County of Guizhou Province. Asian Agric Res. 2018; 10(4):93-98. https://doi.org/10.22004/ag.econ.276163

Yee MM. Investigation of chemical composition, antimicrobial and antioxidant activities of Allium wallichii Kunth. (garlic) bulb. American Scientific Research Journal of Engineering, Technology and Sciences. 2019; 54(1): 30-41.

Midday M, Ghosh J, Pradhan DK, Maity D. Folk-medicinal uses of Allium sikkimense Baker (Alliaceae) in Sikkim Himalaya. Explor Anim Med Res. 2020; 10(2):217-220.

Acharya K, Pokhrel H, Maharjan L, Bhattarai R, Karki P, Shrestha TM, Gyawali R. Comparative study of antibacterial and cytotoxic activity of two Nepalese medicinal plants- Allium wallichii Kunth and Allium sativum L. Int J Pharm Biol Arch. 2011; 2(5):1539-1543.

Pourzand A, Tajaddini A, Pirouzpanah S, Asghari- Jafarabadi M, Samadi N, Ostadrahimi AR, Sanaat Z. Associations between Dietary Allium Vegetables and Risk of Breast Cancer: A Hospital-Based Matched Case-Control Study. J Breast Cancer. 2016; 19(3):292-300. https://doi.org/10.4048/jbc.2016.19.3.292 DOI: https://doi.org/10.4048/jbc.2016.19.3.292

Wang F, Xu Y, Liu P, Li L, Liu Y. The effects of alcohol and water extracts of Allium wallichii Kunth on transplanted hepatoma, lung cancer and breast cancer of mice. Medicinal Plant. 2016; 7(11/12):56-59.

Iqbal J, Abbasi BA, Batool R, Mahmood T, Ali B, Khalil AT, Kanwal S, Shah SA, Ahmad R. Potential phytocompounds for developing breast cancer therapeutics: nature’s healing touch. Eur J Pharmacol. 2018; 827:125-148. https://doi.org/10.1016/j.ejphar.2018.03.007 DOI: https://doi.org/10.1016/j.ejphar.2018.03.007

Arifa F, Fajrina A, Eriadi A, Asra R. Antimicrobial activity test of Genus Allium: A review. Int Res J Pharm. 2020; 11(12):17-25.https://doi.org/10.7897/2230-8407.1112102 DOI: https://doi.org/10.7897/2230-8407.1112102

Paul A, Roy A, Banerjee N. Phylogenetic relationship of some species of Allium L. on the basis of morphological, biochemical and cytological study. Int J Recent Sci Res. 2019; 10[08(B)]:34098-34103. https://doi.org/10.24327/ijrsr.2019.1008.3820

Kumar D, Kumar K, Kumar S, Kumar T, Kumar A, Prakash O. Pharmacognostic evaluation of leaf and root bark of Holoptelea integrifolia Roxb. Asian Pac J Trop Biomed. 2012; 2(3):169-175. https://doi.org/10.1016/S2221-1691(12)60036-7 DOI: https://doi.org/10.1016/S2221-1691(12)60036-7

Killedar SG, More HN, Nadaf SJ. Microscopic Evaluation of Leaves of Memecylon umbellatum Burm. Advances in Agriculture. 2014; 104849. https://doi.org/10.1155/2014/104849 DOI: https://doi.org/10.1155/2014/104849

Akbar S, Hanif U, Ali J, Ishtiaq S. Pharmacognostic studies of stem, roots and leaves of Malva parviflora L. Asian Pac J Trop Biomed. 2014; 4(5):410-415. https://doi.org/10.12980/APJTB.4.2014C1107 DOI: https://doi.org/10.12980/APJTB.4.2014C1107

Yousaf Z, Shinwari ZK, Asghar R, Parveen A. Leaf epidermal anatomy of selected Allium species, family Alliaceae from Pakistan. Pak J Bot. 2008; 40(1):77-90.

Rudall PJ, Chen ED, Cullen E. Evolution and development of monocot stomata. Am J Bot. 2017; 104(8):1122-1141. https://doi.org/10.3732/ajb.1700086 DOI: https://doi.org/10.3732/ajb.1700086