Preliminary Phytochemical Analysis, In-vitro Evaluation of Antibacterial and Anticancer Activity of Cleome aspera Aerial Part Extracts
Authors
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Department of Pharmaceutical Chemistry, Shri Vishnu College of Pharmacy, Vishnupur, Bhimavaram, West Godavari - 534202, Andhra Pradesh ,IN
Anuradha Khuntia -
Department of Pharmaceutical Chemistry, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur – 603203, Tamil Nadu ,INM. K. Kathiravan
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Department of Pharmaceutical Chemistry, Tagore College of Pharmacy, Rathinamangalam, Chennai – 600127, Tamil Nadu ,INK. Ilango
DOI:
https://doi.org/10.18311/jnr/2024/35555Keywords:
Anti-bacterial Activity, Anti-cancer Activity, Cleome aspera, MTT Assay, Phytochemical Screening.Abstract
Various species of the Cleome genus belong to the circle of relatives Cleomaceae family, and order Brassicales, constitute flowering flora with a long record of use in conventional remedies, and have been related to the prevention of many illnesses. Cleome aspera has not been thoroughly investigated in the medicinal area; however, it may offer a therapeutic choice in favour of the management of various disorders. In this recent study, the aerial parts of C. aspera were extracted with petroleum ether, ethyl acetate, and ethanol sequentially in ascending polarity order. Ethanol extract exhibited a rich phytochemical profile and antibacterial and anti-cancer action among the solvent extracts. The phytochemical analysis of three extracts exhibited the existence of many secondary metabolites like flavonoids, alkaloids tannins, polyphenols, steroids, glycosides, and terpenoids. The antibacterial activity against two Gram (+) bacteria, Staphylococcus aureus (ATCC 6538), and Bacillus subtilis (ATCC 6633), as well as two Gram (-) bacteria, Pseudomonas aeruginosa (ATCC 9027) and Escherichia coli (ATCC 8739) was assessed at three concentrations (50, 100, 200 μg/ml) of each extract. The broth dilution method tested the extract’s minimum inhibitory concentration against four bacterial strains. Using the MTT (3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide) assay, the three extracts (petroleum ether, ethyl acetate, and ethanol) of C. aspera were evaluated for in-vitro cytotoxic activity. The extracts were detected to have substantial antibacterial activity against Gram-positive as well as Gram-negative bacteria in a dose-dependent manner. The ethanol extract of C. aspera showed notable anticancer efficacy after comparing it with petroleum ether, and ethyl acetate extracts. This study proved that the plant C. aspera has potential as a source of plant-based drugs and has therapeutic qualities like antibacterial and anticancer activities. It may provide a healing choice for the control of several diseases. A further extensive study on the isolation of active ingredients, purification, characterization, and elucidation of viable mechanisms can be executed.
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Copyright (c) 2024 Mrs, Dr, Dr (Author)
This work is licensed under a Creative Commons Attribution 4.0 International License.
Accepted 2024-01-16
Published 2024-03-27
References
Fabricant DS, Farnsworth NR. The value of plants used in traditional medicine for drug discovery. Environ Health Perspect. 2001; 109(Suppl 1):69-75. https://doi.org/10.1289/ ehp.01109s169 PMid:11250806 PMCid:PMC1240543
Kirtikar KR, Basu BD. Indian medicinal plants (2nd ed). Delhi; M/S Bishen Singh Pal Singh. 1975. p. 1465-72.
Farnsworth NR, Morris RW. Higher plants-farnsworth NR, Morris RW. Higher plants – the sleeping giant of drug development. Am J Pharm Sci Support Public Health. 1976; 148(2):46–52.
Niraimathi KL, Karunanithi M, Brindha P. Phytochemical and in-vitro screening of aerial parts of Cleome viscosa Linn. extracts (Capparidaceae). Int J Pharm Pharm Sci. 2012; 4(2):27–30.
Bose U, Bala V, Ghosh TN, Gunasekhran K, Rehmann AA. Antinociceptive, cytotoxic, and antibacterial activities of Cleome viscose leaves. Rev bras farmacogn. 2011; 21(1):165– 9. https://doi.org/10.1590/S0102-695X2011005000023
Greenwell M, Rahman PKSM. Medicinal plants: their use in anticancer treatment. Int J Pharm Sci Res. 2015; 6(10):4103–12.
Roy A, Bharadvaja N. Medicinal plants in the management of cancer: A review. Int J Complement Alt Med. 2017; 9(1):00291. https://doi.org/10.15406/ijcam.2017.09.00291
Willis JC. A dictionary of the flowering Plant and ferns. Cambridge. The University Press; 1996. p. 64–120.
Raghavan RS, Sharma BD, Balakrishnan NP. Capparaceae In: eds. Flora of India. 1993; 2:248–335.
Pullaiah T. Medicinal plants in India, Vol. 1, New Delhi; Regency Publication, 2006. p. 1-189.
Pullaiah T, Mohammed MS. Flora of Ranga Reddy district Andhra Pradesh. India, first ed, Delhi, Regency Publications; 2000. p. 31–38.
Chetty KM, Sivaji K, Rao T. Flowering plants of Chittoor district, Andhra Pradesh, India, second ed. Tirupati, Students Offset Printers, 2008. p. 61.
Goverdhan P, Murthy EN. Antidiabetic effect of Cleome aspera in type 2 diabetic rats. J Pharm Res. 2009; 2:1072–4.
Tigrine C, Bulzomi P, Leone S, Bouriche H, Kameli A, Marino M. Cleome arabica leaf extract has anticancer properties in human cancer cells. Pharm Biol. 2013; 51:1508–14. https://doi.org/10.3109/13880209.2013.79656 3 PMid:23862683
Singh H, Mishra A, Mishra AK. The chemistry and pharmacology of Cleome genus: A Review. Biomed Pharmacother. 2018; 101:37–48. https://doi.org/10.1016/j. biopha.2018.02.053 PMid:29477056
Mali RG. Cleome viscose (wild mustard): A review on ethnobotany, phytochemistry, and pharmacology. Pharm Biol. 2009; 48(1):105–12. https://doi. org/10.3109/13880200903114209 PMid:20645764
Khuntia A, Martorell M, Ilango K, Bungau SG, Radu AF, Behl T, et al. Theoretical evaluation of Cleome species’ bioactive compounds and therapeutic potential: A literature review. Biomed Pharmacother. 2022; 151:113–61. https:// doi.org/10.1016/j.biopha.2022.113161 PMid:35644118
Mishra SS, Moharana SK, Dash MR. Review on Cleome gynandra. Int J Res Pharm. 2011; 1(3):681–9.
Iyenger MA. Pharmacognosy of crude drugs second ed, KMC Manipal, 1987. p. 21-54.
Dahanukar SA, Kulkarni RA, Rege N. Pharmacognostic study of medicinal plants. Indian J Pharmacol. 2004; 32:82– 118.
Kokate CK. Textbook of Pharmacognosy, fourth ed, New Delhi. Vallabh Prakashan. 1994; 1994. p. 112–20.
Trease GE, Evans E. Pharmacognosy, thirteenth ed. Baillier Tindall. 1989. p. 282-396.
Harborne AJ. Phytochemical Methods - A guide to modern techniques of plant analysis second ed, London. Chapman and Hall. 1998; 1998:54–84.
Quality control methods for medicinal plant materials. Quality Control Methods for Medicinal Plant Materials Organization Mondiale De La Sante. 1992; 9:22–34.
Muhaidat R, Al-Qudah MA, Samir O, Jacob JH, Hussein E, Al-Tarawneh IN, et al. Phytochemical investigation and in-vitro antibacterial activity of essential oils from Cleome droserifolia (Forssk.) Delile and C. trinervia Fresen. (Cleomaceae). S Afr J Bot. 2015; 99:21–8. https://doi. org/10.1016/j.sajb.2015.03.184
Sudhakar M, Rao CV, Rao PM, Raju DB. Evaluation of antimicrobial activity of Cleome viscosa and Gmelina asiatica. Fitoterapia. 2006; 77(1):47–9. https://doi. org/10.1016/j.fitote.2005.08.003 PMid:16325351
Hashem NM, Shehata MG. Antioxidant and antimicrobial activity of Cleome droserifolia (Forssk.) Del. And its biological effects on redox status, immunity, and gut microflora. Animals (Basel). 2021; 11(7):1929. https://doi.org/10.3390/ani11071929 PMid:34203524 PMCid:PMC8300390
Bauer AW, Kirby WMM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966; 45(4_ts):493–6. https://doi. org/10.1093/ajcp/45.4_ts.493 PMid:5325707
Bala A, Kar B, Haldar PK, Mazumder UK, Bera S. Evaluation of anticancer activity of Cleome gynandra on Ehrlich’s Ascites Carcinoma treated mice. J Ethnopharmacol. 2010; 129(1):131–4. https://doi.org/10.1016/j.jep.2010.03.010 PMid:20307641
Suffredini IB, Paciencia M, Varella AD, Younes RN. Invitro cytotoxic activity of Brazilian plant extracts against 628 Preliminary Phytochemical Analysis, In-vitro Evaluation of Antibacterial.... human lung, colon, and CNS solid cancers and leukemia. Fitoterapia. 2007; 78(3):223–6. https://doi.org/10.1016/j. fitote.2006.11.011 PMid:17346903
Soule HD, Vazquez J, Long A, Albert S, Brennan M. A human cell line from a pleural effusion derived from a breast carcinoma 2. J Natl Cancer Inst. 1973; 51(5):1409–16. https://doi.org/10.1093/jnci/51.5.1409 PMid:4357757
Cailleau R, Young R, Olivé M, Reeves WJ Jr. Breast tumor cell lines from pleural effusions. J Natl Cancer Inst. 1974; 53(3):661–74. https://doi.org/10.1093/jnci/53.3.661 PMid:4412247 PMCid:PMC7364228
Joseph M, Vincent AR, Charles A. The anticancer activity of ethanolic extract of Cleome felina Linn. J Pharm Res. 2014; 8(9):1223–5.
Bhandari J, Muhammad BT, Thapa P, Shrestha BG. Study of phytochemical, antimicrobial, antioxidant, and anti-cancer properties of Allium wallichii. BMC Complement Altern Med. 2017; 17(1):1–9. https://doi.org/10.1186/s12906-017- 1622-6 PMid:28178952 PMCid:PMC5299666
Chand J, Panda SR, Jain S, Murty USN, Das AM, Kumar GJ, et al. Phytochemistry and polypharmacology of cleome species: A comprehensive Ethnopharmacological review of medicinal plants. J Ethnopharmacol. 2022; 282(114600):114600. https:// doi.org/10.1016/j.jep.2021.114600 PMid:34487845
Ahouansinkpo E, Atanasso J, Dansi A, Adjatin A, Azize O, Sanni A. Ethnobotany, phytochemical screening and toxicity risk of Cleome gynandra and Cleome viscosa, two traditional leafy vegetables consumed in Benin. Int J Curr Microbiol Appl Sci. 2016; 5(2):813–29. https://doi. org/10.20546/ijcmas.2016.502.093
Adhikari PP, Paul SB. Medicinally important plant Cleome gynandra: A phytochemical and pharmacological explanation. Asian J Pharm Clin Res. 2018; 11(1):21. https:// doi.org/10.22159/ajpcr.2018.v11i1.22037
Naeem H, Perveen R, Zaidi S, Fatima ZZ, Akram K, Hussain Z, et al. Cleome brachycarpa: A Review on Ethnobotany, Phytochemistry, and Pharmacology. Phytochemistry, and Pharmacology RADS J Pharm Pharm Sci. 2019; 7(2):107– 11.
El Amine Dib M, Allali H, Bendiabdellah A, Meliani N, Tabti B. Antimicrobial activity and phytochemical screening of Arautus unedo L. J Saudi Chem Soc. 2013; 17:381–5. https:// doi.org/10.1016/j.jscs.2011.05.001
Hussain J, Khan H, Ali L, Latif Khan A, Ur Rehman N, Jahangir S, et al. ChemInform Abstract: A New Indole Alkaloid from Cleome droserifolia. ChemInform. 2015; 46(42):46-52. https://doi.org/10.1002/chin.201542205
Venkatadri B, Arunagirinathan N, Rameshkumar MR, Ramesh L, Dhanasezhian A, Agastian P. In-vitro antibacterial activity of aqueous and ethanol extracts of Aristolochia indica and Toddalia asiatica against multidrugresistant bacteria. Indian J Pharm Sci. 2015; 77(6):788–91. https://doi.org/10.4103/0250-474X.174991 PMid:26997710 PMCid:PMC4778242
Abate L, Tadesse MG, Bachheti A, Bachheti RK. Traditional and phytochemical bases of herbs, shrubs, climbers, and trees from Ethiopia for their anticancer response. Biomed Res Int. 2022; 2022:1589877. https://doi.org/10.1155/2022/1589877 PMid:35155671 PMCid:PMC8831057
Senthamilselvi MM, Kesavan D, Sulochana N. An antiinflammatory and anti-microbial flavone glycoside from flowers of Cleome viscosa. Org Med Chem Lett. 2012; 2(1):19. https://doi.org/10.1186/2191-2858-2-19 PMid:22613049 PMCid:PMC3493290
Ezzat SM, Abdel Motaal A. Isolation of new cytotoxic metabolites from Cleome droserifolia growing in Egypt. Z Naturforsch C. 2012; 67(5–6):266–74. https://doi. org/10.1515/znc-2012-5-605 PMid:22888531
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