Unlocking Bioactive Potential: A Comparative Analysis of Solvent Extraction on Phytochemicals and Antimicrobial Efficacy in Eupatorium glandulosum and Eupatorium odoratum

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Authors

  • Nirza Moktan
     Department of Microbiology, Dhruba Chand Halder College, Dakshin Barasat - 743372, West Bengal ,IN
  • Tapan Seal
     Plant Chemistry Department, Botanical Survey of India, Shibpur, Howrah - 711103, West Bengal ,IN
  • Anindita Banerjee
     Department of Microbiology, St. Xavier’s College (Autonomous), Kolkata - 700016, West Bengal ,IN

DOI:

https://doi.org/10.18311/jnr/2024/35727

Keywords:

Antimicrobial, Antioxidant, Ethnomedicine, Eupatorium, Phytocompounds, Vitamins

Abstract

The purpose of this work was to explore and compare the phytochemical constitution, vitamin composition, antioxidant activity, and antimicrobial potential of leaf extracts from two Ethnomedicinal plants, Eupatorium glandulosum and  Eupatorium odoratum, belonging to family Asteraceae collected from the Eastern Himalayan Darjeeling region and the plains of Jhargram in West Bengal, India. Four distinct solvents viz., 80 % aqueous ethanol, ethyl acetate, chloroform and n-hexane were used to prepare the leaf extracts. The identification and quantification of phytochemicals and water-soluble vitamins was conducted through HPLC. To evaluate antioxidant potential, 2,2-Diphenyl-1-picryl hydrazyl (DPPH), 2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS), and Ferric Reducing Antioxidant Power (FRAP) assays were performed. The antimicrobial activity was investigated against Streptocococcal strains (S. oralis, S. mutans, S. pyogenes). Both the plants exhibited a diverse array of identified phytochemicals. E. glandulosum contained five while E. odoratum presented an even richer composition of seven water-soluble vitamins. Remarkably, E. odoratum displayed the highest scavenging activity against DPPH radicals (70.08%), and notable reducing potential (10.74 AAE mg/gm). In contrast, E. glandulosum showcased pronounced scavenging potential against ABTS radicals (74.32%). Additionally, the ethyl acetate extract from E. odoratum displayed robust antimicrobial efficacy against, S. oralis, inducing an inhibition zone of 16.00 mm ± 2.82 while E. glandulosum exhibited an inhibition zone of 11.50 mm ± 0.707. These findings validate the therapeutic potential and ethnomedicinal use of these plants.

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Published

2024-05-01

How to Cite

Moktan, N., Seal, T., & Banerjee, A. (2024). Unlocking Bioactive Potential: A Comparative Analysis of Solvent Extraction on Phytochemicals and Antimicrobial Efficacy in <i>Eupatorium glandulosum</i> and <i>Eupatorium odoratum</i>. Journal of Natural Remedies, 24(5), 1061–1073. https://doi.org/10.18311/jnr/2024/35727

Issue

Volume 24, Issue 5, May 2024

Section

Research Articles

Categories

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Copyright (c) 2024 Nirza Moktan, Tapan Seal, Anindita Banerjee (Author)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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Europe PMC
Received 2023-11-29
Accepted 2024-03-13
Published 2024-05-01

 

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