Hepatoprotective Effect of Ethanolic Extract of Whole Plant of Tephrosia barberi J. R. Drumm. in Paracetamol Induced Hepatotoxicity in Rats
Authors
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Karpagam Academy of Higher Education, Coimbatore - 641021, Tamil Nadu ,IN
S. Sameemabegum -
Karpagam Academy of Higher Education, Coimbatore - 641021, Tamil Nadu ,INRamasamy Thilagavathi
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Karpagam Academy of Higher Education, Coimbatore - 641021, Tamil Nadu ,INP. Kumarnallasivan
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Nandha College of Pharmacy, Erode - 638052, Tamil Nadu ,INThangavel Sivakumar
DOI:
https://doi.org/10.18311/jnr/2024/36162Keywords:
Acute Toxicity, GC-MS, Hepatoprotective, Phytochemical Analysis, Tephrosia barberiAbstract
Background: Tephrosia barberi J. R. Drumm is a novel endemic perennial herb belonging to the Fabaceae family. Aim: To investigate the hepatoprotective activity of the whole plant of T. barberi. Methods: Preliminary phytochemical testing and GCMS analysis were done for the ethanolic extract of T. barberi. Initially, acute toxicity experiments were carried out in which five groups of rats (n=3) were administered with 0.5% carboxymethyl cellulose (normal control), ethanolic extract of T. barberi orally (5, 50, 300 and 2000 mg/kg b.w.) for 14 days daily, there was no mortality observed for 24 hr. For the assessment of the hepatoprotective potential, ethanolic extracts of T. barberi (200mg/kg low dose and 400mg/kg high dose) and silymarin (100mg/kg, employed as the reference standard), distilled water (normal control, paracetamol control) was given orally to five groups of rats (n=6) over seven days. All animals were given paracetamol on the fifth day, following the administration of standard and extracts, except for those in normal control. To assess the levels of serum enzymes, proteins, antioxidant indicators, and histopathological analyses, the rats were sacrificed and blood samples, liver homogenate, and liver sections were taken. Results: Rats fed with T. barberi ethanol extract (400mg/kg/day) and silymarin, a standard drug (100mg/kg/day) treated groups had significantly elevated liver serum enzymes such as alanine phosphatase, aspartate aminotransferase, alanine transaminase, total protein and total bilirubin than the intoxicated rats (p<0.01to p<0.001). Furthermore, the effect of antioxidant parameters (GSH, CAT, and SOD) on hepatic tissue showed that ethanol extract of T. barberi (high dose, 400mg/kg) produced improved hepatic function than toxic control rat and exhibited significant action compared to standard drug. Conclusion: The current research findings strongly indicate that T. barberi has promising hepatoprotective potential due to the presence of flavonoids and phenolic compounds.
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Copyright (c) 2024 S. Sameemabegum, Ramasamy Thilagavathi, P. Kumarnallasivan, Thangavel Sivakumar (Author)
This work is licensed under a Creative Commons Attribution 4.0 International License.
Accepted 2024-06-17
Published 2024-07-31
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