Standardisation and Quality Control of Herbal Drugs-1 TLC Fluorodensitometric Determination of Marker Compounds of Plumbago indica L.


  • University of Sri Jayewardenepura, Department of Chemistry, Nugegoda, Sri Lanka
  • Link Natural Products Ltd., Research Laboratory, Kapugoda, Sri Lanka


Objective: Develop a method to quantify a mixture of isoshinanolone and epi- isoshinanolone (ISMIX) as a specific marker for Plumbago indica L. in a commercial herbal drug, "Dasamoolarishtaya" (DMA). Materials and Methods: The ISMIX content in different DMA samples were analyzed by TLC fluorodensitometry. Isoshinanolone isolated from the root of P.indica was used as the standard. The precision and accuracy of the method were determined. Results: The concentrations of ISMIX in different samples of DMA varied widely from < 0.0012 mg/ml (detection limit of method) to 0.045 mg/ml. The precision was 1.52 % CV (n = 4) and recoveries of 99.2 - 100.8 % were obtained in standard addition recovery experiments. Conclusions: The method described is inexpensive, rapid and is of acceptable precision and accuracy for routine quality control work.


Fluorodensitometry, Isoshinanolone, Epi- Isoshinanolone, Quality Control, Standardization, Herbal Drugs.

Subject Discipline

Pharmacy and Pharmacology

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