Toxicity of Millettia ferruginea (Hochst) Baker against the Larvae and Adult Ticks of Boophilus decoloratus a One-Host Tick in Cattle


  • Dilla University, Department of Chemistry, Dilla, Ethiopia


The present article reports the in vitro toxicity of the seed of Millettia ferruginea (Berbera) for the first time against the larvae and mature ticks of Boophilus decoloratus. The 20%, 40%, 60%, 80% and 100% concentrations of the seed oil were found to kill all (100% mortality) larvae after 16hr, 12hr, 8hr, 6hr and 2hr respectively. The 100% concentration of the petroleum extract (oil) of the seed caused 100% mortality of adult male, adult female and fully engorged female tick after 5hr, 7hr and 12hr respectively. The root and root bark showed less toxicity while leaves did not show any activity. Among petroleum ether, acetone and methanol, the acetone extract showed highest acaricidal activity.   


Acaricide, Boophilus decoloratus, Cattle, Fabaceae, In vitro, Millettia ferruginea, Tick

Subject Discipline

Pharmacy and Pharmacology

Full Text:


Bekele A. Useful trees and shrubs for Ethiopia: Identification, propagation and Management for 17 agro climatic zones, Nairobi: RELMA in ICRAF project, Nairobi, Kenya. 2007.

Karunamoorthi K, Bishaw D, Mulet T. Toxic effects of traditional Ethiopian fish poisoning plant Millettia ferruginea (Hochst) seed extract on aquatic macro invertebrates. European Review for Medical and Pharmacological Sciences. 2009; 13:179–85.

Teketay D, Tegineh A. Shade trees of coffee in Harare, Eastern Ethiopia. The International Tree Crops Journal. 1991; 7:17–27.

Tadesse H, Legesse N, Olsson M. Mellittia ferruginea from Southern Ethiopia: impacts on soil fertility and growth of maize. Agroforestry Systems. 2000; 48:9–24.

Azene B. Useful trees and shrubs of Ethiopia; identification, propagation and management for 17 agroclimatic zones. In Technical Manual No.6. RELMA in ICRAF Project Kenya. 2007. p. 550. ISBN: 978-92905-92129.

Mesfin F, Demissew S, Telehaymanot T. An ethno botanical study of medicinal plants of Wonago, Woreda, SNNPR, Ethiopia. J Ethno Biol and Ethno Med. 2009; 5(28):1–8.

Teklelehaymanot T, Giday M. Ethno Botanical study of medicinal plants used by people in Zeige Peninsula, North-western Ethiopia. J Ethno Biol Ethno Med. 2007; 3(12):1–12.

Damte T, Chichaybelu M. The efficacy of some botanicals in controlling Adzuki bean beetle, Callosobruchus chinensis in stored chickpea. Tropical Sci. 2002; 42:192–5.

Jembere B. Evaluation of the toxicity potential Millettia ferruginea (Hochest) Baker against Sitophilus zeamais Mots. International J Pest Management. 2002; 42(1):29– 32.

Jembere B, Namukobe J, Benard TK, Dagne E. Extracts of Millettia ferruginea, Tephrosia vogellii and Tephrosia pentaphylla against the bean weevil Zabrotes subfasciatus (Boheman). Sinet: Ethiopian J Sci. 2007; 30:49–54.

Tilahun B, Azerefegne F. Efficacy of the aqueous crude seed extract of Millettia ferruginea (Fabaceae) on the maize stemborer Busseola fusca (Lepidoptera: Noctuidae) in the field. International J Tropical Insect Sci. 2013; 33(4):256–63.

Getahun D, Jembere B. Evaluation of toxicity of crude extracts of some botanicals on different castes of Macrotermes termites. Paste Management Journal of Ethiopia. 2006; 10:15–23.

Shaw RD, Thorburn JA, Wallace HG. Cattle Tick Control, Prepared by Members of The Wellcome Research Organisation: Cooper Division Wellcome Foundation Ltd, London, England. 1970. p. 15.

Soulsby EJL. Helminths, Arthropods and Protozoa of Domesticated Animals, 7th edition, English Language Book Society (ELBS)/Bailliere Tindall, London, England. 1982. p. 468.

Ndumu AA, George JBD, Choudhury MK. Toxicity of neem seed oil (Azadirachta indica) against the larvae of Amblyomma variegatum a three-host tick in cattle. Phytotherapy Research. 1999; 13:532–4.

Choudhury MK. Toxicity of neem seed oil (Azadirachta indica) against the larvae of Rhipicephalus sanguineus a three-host tick in dog. J Parasitic Diseases. 2001; 25(11): 46–7.

Choudhury MK. Efficacy of neem leaf (Azadirachta indica, family: Meliaceae) in the Control of larvae of Boophilus decoloratus, a one-host tick in cattle. J Nepal Pharm Assocn. 2003; 21(1):41–3.

Choudhury MK. Toxicity of neem seed oil against the larvae of Boophilus decoloratus, a one-host tick in cattle. Indian J Pharm Sci. 2009 Sep-Oct; 562–3.

Choudhury MK, Boshe FD. Toxicity of Khaya senegalensis seed oil against the larvae of Boophilus decoloratus: a onehost tick in cattle. Proceedings of Zoological Soc. 2013; 66(2): 137–40. Published online 17 Jan. DOI: 10.1007/ s12595-012-0058-8.

Clark EP. The occurrence of rotenone and related substances in the seeds of the Berebera tree. A procedure for the separation of deguelin and tephrosin. J Am Chem Soc. 1943; 65:27–9.

Highet RJ, Highet PF. The structure of two isoflavones from the Abyssinian Berebera tree. J Org Chem. 1967; 32:1055–8.

Vilain C. Barbigerone, a new pyranoisoflavone from seeds of Tephrosia barbigera. Phytochemistry. 1980; 19:988–9.

Dagne E, Bekele A, Waterman PG. The flavonoids of Millettia ferruginea subsp. Ferruginea and subsp. Darassana in Ethiopia. Phytochemistry. 1989; 28(7):1897–900.

Dagne E, Bekele A, Noguchi H, Shibuya M, Sankawa U. O-geranylated and O- prenylated flavonoids from Millettia ferruginea. Phytochemistry. 1990; 29(8):2671–73.

Dagne E, Bekele A. C-prenylated isoflavones from Millettia ferruginea. Phytochemistry. 1990; 29(8):2679–82.

Choudhury MK, Shiferaw Y, Hussen A. Toxicity of Millettia ferruginea darasana (family: Fabaceae) against the larvae and adult ticks of Amblyomma variegatum Fabricius, a three host tick in cattle. J. Parasitic Diseases. 2013; 39:298–302. DOI: 10.1007/s12639-013-0311-8.


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