Cytological Assessment of Barr Body in Buccal Scrapes: A Comparative Study
Keywords:Barr body, Carbol fuchsin, Papanicolaou, Acridine orange
AbstractIntroduction: Barr body is a deeply stained condensed chromatin material seen in the nuclei of females, which is taken as a vital tool in the identification and determination of sex of the individual. Various staining techniques can be used for their demonstration. However, staining with carbol fuchsin, papanicolaou and acridine orange were considered appropriate as they produce stronger contrast and are simple to use.
Aim: To determine the sex and also to assess the accuracy and efficacy of special nuclear stains like carbol fuchsin, papanicolaou and acridine orange in staining Barr bodies.
Materials and methods: A total of 300 samples were included in the present study. The smears were collected and subsequently stained with three different special stains of which 100 samples were stained with acridine orange, 100 with papanicolaou and 100 with carbol fuchsin. Stained smears were examined for Barr bodies and also for their staining accuracy and efficacy.
Results and discussion: Barr body was found to be negative in the male samples and positive for female samples with acridine orange yielding 64%, papanicolaou yielding 46.14% and carbol fuchsin 8.68% of Barr-body-positive cells.
Conclusion: On comparison, the efficacy and accuracy for detection of Barr bodies were more with acridine orange followed by papanicolaou and carbol fuchsin.
Datar U, Angadi PV, Hallikerimath S, Kale AD. Cytological assessment of barr bodies using aceto-orcein and papanicolaou stains in buccal mucosal smears and their sex estimation efficacy in an Indian sample. Acta Cytol. 2013;57:516–521.
Khanna KS. Efficacy of sex determination from human dental pulp tissue and its reliability as a tool in forensic dentistry. J Int Oral Health. 2015;S:1–7.
Reddy DS, Sherlin HJ, Ramani P, Prakash PA. Determination of sex by exfoliative cytology using acridine orange confocal microscopy: a short study. J Forensic Dent Sci. 2012;4:66–69.
Meena DNK, Singh DM, Singh DJ. A comparison study of carbol fuchsin and papanicolaou staining methods for the demonstration and enumeration of barr bodies in buccal smear. IJAR. 2016;2:941–944.
Lyon MF. X-chromosome inactivation and human genetic disease. Acta Paediatr. 2002;91(suppl):107.
Mittwoch U. Sex chromatin. J Med Genet. 1964;1:50–76.
Davis AM, Herrmann W. The determination of chromosomal sex by oral smears. Yale J Biol Med. 1956;29:69–74.
Manjula Bhai KH, Yadwad BS, Patil PV. A study of Barr bodies in Indian, Malaysian and Chinese subjects. J Forensic Med Toxicol. 1997;14:9–13.
Suazo GI, Roa HI, Cantin LM. Sex chromatin in dental pulp: performance of diagnosis test and gold standard generation. Int J Morphol. 2010;28:1093–1096.
Galdames IS, Flores A, Roa I, Cantin M, Zavando D. Sex determination by observation of Barr body in teeth subjected to high temperature. Int J Morphol. 2011;29:199–203.
Verma U, Chowdhary DS, Chhabra S. Sex chromatin positive cells in the buccal smears of normal newborn females. Int J Biol Med Res. 2013;4:3317–3319.