Chemomodulatory Effect of Trigonella foenum graecum (L.) Seed Extract on Two Stage Mouse Skin Carcinogenesis


  • The IIS University, Mansarovar, Department of Biotechnology, Jaipur, Rajasthan, India
  • University of Rajasthan, Cell and Molecular Biology Laboratory,Department of Zoology, Jaipur, Rajasthan, India
  • University of Rajasthan, Radiation and Cancer Biology Laboratory,Department of Zoology, Centre for Advanced Studies, Kanpur, Uttar Pradesh, India


Cancer is not a single disease but a group of complex genetic diseases of aged cells. Chemoprevention of cancer is the attempt to use natural and synthetic compounds to intervene in the early stages of cancer, before invasive disease begins. Consuming a diet rich in plant foods can provide a milieu of phytochemicals and non-nutritive plant substances that possess health-protective effects. Some phytochemicals derived in spices and herbs as well as other plants possess substantial cancer preventive properties. Thus the cancer chemo preventive potential of naturally occurring phytochemicals is of great interest because of their preventive role and as they are not perceived as “medicineâ€. During the course of present study Trigonella foenum graecum (L.) seed- TFGS (commonly called fenugreek) extract was given at pre-initiational, post-initiational, promotional and throughout the experiment along with 7,12-dimethylbenz [a] anthracene DMBA and 12-O-tetradecanoylphorbol-13-acetate TPA treatment in Swiss albino mice. A significant reduction of papillomas in DMBA + TPA + TFGS (400 mg/kg. body wt.) treated group was found to be effective in decreasing the rate of tumor incidence in comparison to control. Furthermore, cumulative number of papillomas, tumor yield and tumor burden were also found to be reduced. The TFGS extract treatment before DMBA and TPA application (i.e. Pre initiation) were more effective than that of treatment during, and /or after DMBA treatment, however TFGS extract treatment was most effective when treated throughout all the stages of tumorigenesis. The TFGS treatment also showed a modulatory influence on mouse hepatic antioxidant defense system (GSH and LPO level).


Cancer, chemoprevention, DMBA, phytochemicals, TPA, Trigonella foenum graecum

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Shukla S, Gupta S. Apigenin: A promising molecule for cancer prevention. Pharm Res 2010;27:962-78.

Oliveria SA, Christos PJ, Berwick M. The role of epidemiology in cancer prevention. Proc Soc Exp Biol Med 1997;216:142-50.

Martinez M, Giovanucci E. Diet and the prevention of cancer.Cancer Metastasis Rev 1997;16:357-76.

World Cancer Research Fund. Food, nutrition and the prevention of cancer: A global perspective. Washington, DC: American Institute for Cancer Research; 1997.

Greenwald P, Kelloff G, Burch-Whitman C. Chemoprevention.CA Cancer J Clin 1995;45:31-49.

Lampe JW. Health effects of vegetables and fruit: Assessing mechanisms of action in human experimental studies. Am J Clin Nutr 1999;70:475S-90S, Review.

Aggarwal BB, Shishodia S. Molecular target of dietary agents for prevention and therapy of cancer. Biochem Pharmacol 2006;71:1397-421.

Surh YJ. Anti-tumor promoting potential of selected spice ingredients with antioxidative and anti-inflammatory activities: A short review. Food Chem Toxicol 2002;40:1091-7.

Surh YJ. Cancer chemoprevention with dietary phytochemicals.Nat Rev Cancer 2003;3:768-80.

Lai PK, Roy J. Antimicrobial and chemopreventive properties of herbs and spices. Curr Med Chem 2004;11:1451-60.

Aggarwal BB, Kunnumakkara AB, Harikumar KB, Tharakan ST, Sung B. Potential of spice derived phytochemicals for cancer prevention. Planta Med 2008;74:1560-9.

Kelloff GJ, Boone CW, Crowell JA, Steele VE, Lubet R, Sigman CC.Chemopreventive drug development: Perspectives and progress.Cancer Epidemiol Biomarkers Prev 1994;3:85-98.

Wattenberg LW. Chemoprevention of cancer. Prev Med 1996;25:44-5.

Mayne S, Lippman S. Cancer prevention: Chemopreventive agents. In: DeVita V, Hellman S, Rosenberg S, editors. Cancer: Principles and Practices of Oncology. Vol 5. Philadelphia, PA: Lippincott-Raven Publishers; 1997. p. 585-99.

Kelloff GJ, Hawk ET, Karp JE, Crowell JA, Boone CW, Steele VE, et al. Progress in clinical chemoprevention. Semin Oncol 1997;24:241-52.

Goodman G. Cancer prevention: Contrasting dietary modification with intervention agents. Encycl Cancer 1997;1:199-206.

Singletary K. Diet, natural products and cancer chemoprevention.J Nutr 2000;130:465S-66S.

Slattery ML, O’Brien E, Mori M. Disease heterogeneity: Does it impact our ability to detect dietary associations with breast cancer? Nutr Cancer 1995;24:213-20.

Barone M, Sofano K, Tullio ND, Licino R, Albano F, Dileo A. Dietary, endocrine and metabolic factors in tje development of colorectal cancer. J Gastrointest Cancer 2012;43:13-9.

Chevallier A. Encyclopedia of herbal medicine. New York (NY): Dorling Kindersley Publishing, Inc.; 2000. p. 271.

Hedrick UP. Sturtevant’s edible plants of the world. ISBN 0-48620459-6. New York: Dover Publications; 1972.

Harrison S, Wallis M, Masefield G. The oxford book of food plants.Oxford University Press; 1975.

Lust J. The Herb Book. Bantam books. USA: Benedict Lust Publication; 1983.

Grieve. A modern herbal.In:Leyel CF, Editor. Great Britain: Penguin Publisher; 1984

Phillips R, Foy N. Herbs. ISBN 0-330-30725-8. London: Pan Books Ltd.; 1990.

Qiblawi S, Kumar A. Chemopreventive action by an extract from Brassica compestris (var sarason) on 7, 12-dimethylbenz (a) anthracene induced skin papillomagenesis in mice. Phytother Res 1999;13:261-3.

Moron MA, Depierre JW, Mannervik B. Levels of GSH, GR and GST, in rat lung and liver. Biochem Biophys Acta 1979;582:67-78.

Ohkhawa H, Ohishi N, Yogi K. Assay for lipid peroxidation in animal tissue by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.

Shishodia S, Aggarwal BB. Diosgenin inhibits osteoclastogenesis, invasion, and proliferation throughthe downregulation of Akt, IkappaB kinase activation and NF-kappaB-regulated gene expression. Oncogene 2006;25:1463-7.

Patel K, Godewar M, Tahilyani V, Patel DK. A Review on pharmacological and analytical aspects of diosgenin: A concise report. Nat Prod Bioprospect 2012;2:46-52.

Levy J, Teuerstein L, Marbach M, Radian S, Sharoni Y. Tyrosine protein kinase activity in the DMBA-induced rat mammary tumor: Inhibition by quercetin. Biochem Biophys Res Commun 1984;725:1227-33.

Afaq F, Katiyar SK. Dietary phytochemicals and chemoprevention of solar ultraviolet radiation induced skin cancer. In: Sarkar FH, Editor.Neutraceuticals and Cancer,1st Edn.USA: Springer Publishers; 2012;259-321.

In: Mazza G, Oomah BD, editors. Chemistry and pharmacology of fenugreek. Herbs, botanicals and teas. Lancaster, USA: Technomic Publishing Co.; 2000.

Gupta M, Shaw BP. Uses of medicinal plants in Panchakarma Ayurvedic therapy. Indian J Traditional Knowledge 2009;8:372-8.

Thiele JJ, Schroeter C, Hsieh SN, Podda M, Packer L. The antioxidant network of the stratum corneum. Curr Probl Dermatol 2001;29:26-42.

Mandelker L. Oxidative stress, free radicals, and cellular damage. Oxidat Stress Appl Basic Res Clin Pract 2011;5:1-17.

Ketterer B. Glutathione S-Transferase and prevention of cellular free radical damage. Free Radic Res 1998;28:647-58.

Seo KW, Kim GJ, Park M, Kim TW, Kim HJ. Effects of phenethylisothiocyanate on the expression of glutathione S transferase and hepatotoxicity induced by acetaminophen.Xenobiotica 2000;30:535-43.

Ursini F, Maiorino M, Sevanian A. Membrane hydroperoxides. In: Oxidative stress: Oxidants and antioxidants. In: Sies H, editor.London: Harcourt Brace Jovanovich; 1991. p. 319-36.

Krishnaswamy K, Raghuramulu N. Bioactive phytochemicals with emphasis on dietary practices. Indian J Med Res 1998;108:167-81.

Raju J, Patlolla JM, Swamy MV, Rao CV. Diosgenin, a steroid saponin of Trigonella foenum graecum (fenugreek), inhibits azoxymethane-induced aberrant crypt foci formation in f344 rats and induces apoptosis in ht-29 human colon cancer cells. Cancer Epidemiol Biomarkers Prev 2004;13:1392-8.

Wattenberg L. Chemoprevention of cancer. Cancer Res 1985;45:1-6.


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