Evaluation of the Antibacterial Activity of Isolated Anthraquinones from the Root of <i>Rubia cordifolia</i> Linn


  • Pottella Srinivasulu Acharya Nagarjuna University, Guntur – 522510, Andhra Pradesh
  • Padarthi Pavan Kumar Department of Physiology and Pharmacology, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram – 522019, Andhra Pradesh
  • Ch Aruna Kumar Department of Physiology and Pharmacology, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram – 522019, Andhra Pradesh
  • J. Venkateswara Rao Department of Pharmaceutics, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram – 522019, Andhra Pradesh
  • S. Vidyadhara Department of Pharmaceutics, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram – 522019, Andhra Pradesh
  • Janga Ramesh Babu Department of Pharmaceutics, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram – 522019, Andhra Pradesh




MIC, Micro Broth Dilution, Quinizarine, 2-Methyl Anthraquinone


The present study was intended to screen the antibacterial activity of anthraquinones i.e. 2-Methyl Anthraquinone (MAQ) and Quinizarine (QNZ), isolated from roots of Rubia cordifolia Linn. Minimum Inhibitory Concentration (MIC) of isolated anthraquinones was evaluated against selected gram-positive [Bacillus subtilis (BS) and Staphylococcus aureus (SA)] and gram-negative [Serratia marcescens (SM) and Escherichia coli (EC)] bacterial strains. The MIC was determined by micro broth dilution method using Tryptone Soya Broth (TSB) in 96 well plates as well as by measuring zone of inhibition using nutrient agar medium by the cup-plate method. Absorbance values after micro broth dilution revealed the significant MIC of MAQ as 40 μg/mL against BS, 40 μg/mL of MAQ against SA and 80 μg/mL of MAQ against SM. Whereas, The MIC of QNZ was 20 μg/mL against EC. Percentage zone of inhibition after cup-plate method revealed the significant MIC of MAQ as 39.8% against BS when compared with vancomycin, 88.33% against SA when compared with linezolid, 53.37% against SM when compared with amikacin. Whereas, percentage zone of inhibition revealed the significant MIC of QNZ as 53.76% against EC when compared with ciprofloxacin. Based on the MIC results after both micro broth dilution and cup-plate method, we observed that MAQ has shown considerable antibacterial activity against selected bacteria.


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How to Cite

Srinivasulu, P., Pavan Kumar, P., Aruna Kumar, C., Venkateswara Rao, J., Vidyadhara, S., & Ramesh Babu, J. (2022). Evaluation of the Antibacterial Activity of Isolated Anthraquinones from the Root of <i>Rubia cordifolia</i> Linn. Toxicology International, 27(3&amp;4), 193–201. https://doi.org/10.18311/ti/2020/v27i3&4/26169



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