Effects of Caffeine on the Fertility of <i>Hermetia Illucens</i> (Stratiomyidae), fed with Coffee Pulp


  • Michelle Alexandra Pardo Department of Biology, Faculty of Natural and Exact Sciences, Universidad del Valle, Santiago de Cali - 760032
  • Nancy Soraya Carrejo Gironza Department of Biology, Faculty of Natural and Exact Sciences, Universidad del Valle, Santiago de Cali - 760032




Black Soldier Fly, Caffeine, Coffee Pulp


Coffee Pulp is a residue with high concentrations of caffeine, which is a harmful alkaloid that affects fertility in Diptera. The main objective of this study is to define how the caffeine present in the Coffee Pulp affects the fertility of H. illucens, seeking to establish if the bioconversion of Coffee Pulp affects the long-term reproduction of adults. For this purpose, larvae were fed with fresh Coffee Pulp and solid vegetable waste. The pre-pupae obtained were weighed and the internal genitalia of 90 females from each diet were dissected. Afterwards, crosses were conducted between the adults from each diet, the egg clutches were weighed and the emerged larvae were counted. We encountered that the Coffee Pulp does not affect the adults' survival or the weight of the pre-pupae, but it does affect the developmental duration of the immature state. No significant differences were found in the oocyte production between diets, but their size varied according to the females' age. We found that the hatching rate varied significantly between the crosses and the control. With this study we can affirm that caffeine does not affect the fertility of H. illucens, but we cannot affirm that it affects the hatching rate. The economic and environmental impact of our results is significant, due to the potential that H. illucens has in the bioconversion of Coffee Pulp and the commercial use of the byproducts.


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How to Cite

Alexandra Pardo, M., & Soraya Carrejo Gironza, N. (2022). Effects of Caffeine on the Fertility of <i>Hermetia Illucens</i> (Stratiomyidae), fed with Coffee Pulp. Toxicology International, 27(3&amp;4), 202–210. https://doi.org/10.18311/ti/2020/v27i3&4/26234



Research Articles