Microbial Decolorization of Crystal Violet Dye by a Native Multi-Metal Tolerant Aeromonas caviae MT-1 Isolate from Dye-Contaminated Soil: Optimization and Phytotoxicity Study

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Authors

  • Manikant Tripathi
     Biotechnology Program, Dr. Rammanohar Lohia Avadh University, Ayodhya - 224001, Uttar Pradesh ,IN
  • Neelam Pathak
     Department of Biochemistry, Dr. Rammanohar Lohia Avadh University, Ayodhya - 224001, Uttar Pradesh ,IN
  • Vinod Kumar Chaudhary
     Department of Environmental Sciences, Dr. Rammanohar Lohia Avadh University, Ayodhya - 224001, Uttar Pradesh ,IN
  • Pankaj Singh
     Biotechnology Program, Dr. Rammanohar Lohia Avadh University, Ayodhya - 224001, Uttar Pradesh ,IN
  • Pradeep Kumar Singh
     Department of Biochemistry, Dr. Rammanohar Lohia Avadh University, Ayodhya - 224001, Uttar Pradesh ,IN
  • Banjagere Veerabhadrappa Thirumalesh
     Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Thiruvananthapuram - 695019, Kerala ,IN
  • Saroj Bala
     Department of Microbiology, Punjab Agriculture University, Ludhiana - 141001, Punjab ,IN
  • Avnish Kumar Maurya
     Department of Biochemistry, Dr. Rammanohar Lohia Avadh University, Ayodhya - 224001, Uttar Pradesh ,IN
  • Naveen Patel
     Department of Civil Engineering, IET, Dr. Rammanohar Lohia Avadh University, Ayodhya - 224001, Uttar Pradesh ,IN
  • Brijesh Kumar Yadav
     Department of Environmental Sciences, Dr. Rammanohar Lohia Avadh University, Ayodhya - 224001, Uttar Pradesh ,IN

DOI:

https://doi.org/10.18311/ti/2023/v30i1/31254

Keywords:

Aeromonas caviae, Crystal Violet Dye, Decolorization, Heavy Metals, Phytotoxicity, Waste Treatment

Abstract

In the recent past, one of the main environmental issues is the contamination of textile dye wastes. The toxicity of dyes poses adverse effects on the flora and fauna of the ecosystem. The present study aimed to isolate bacteria that decolourize crystal violet dye, optimization of various environmental factors for effective decolourization, and phytotoxicity analysis. Out of 13 isolated bacteria, a single isolate was able to grow at 250 mg/L crystal violet dye concentration in a synthetic medium and identified as Aeromonas caviae MT-1 strain (accession number; LC720408) using morphological, biochemical and molecular analyses. Presumably, this is the first report of crystal violet dye decolourization by a native Aeromonas caviae isolate. In this study, after a 72-hour incubation period, a maximum of 98.0% dye decolourization was observed at neutral pH and 35°C with 5% v/v bacterial culture under static culture conditions. Dye decolourization was inhibited to a significant degree by the rising of its concentration. UV-Vis spectra analysis of samples before and after decolourization showed the possible degradation of crystal violet dye by A. caviae. The strain MT-1 was also tolerant to toxic heavy metals like arsenic, lead, and chromium. Phytotoxicity tests revealed that decolourized dye products inhibited Vigna radiata growth less than the un-decolourized dye solution. The findings revealed that a native multi-metal tolerant A. caviae MT-1 isolate could decolourize crystal violet dye rapidly, and possibly have the ability for extensive treatment of dyecontaminated waste.

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Published

2023-03-20

How to Cite

Tripathi, M., Pathak, N., Chaudhary, V. K., Singh, P., Singh, P. K., Thirumalesh, B. V., Bala, S., Maurya, A. K., Patel, N., & Yadav, B. K. (2023). Microbial Decolorization of Crystal Violet Dye by a Native Multi-Metal Tolerant <i>Aeromonas caviae</i> MT-1 Isolate from Dye-Contaminated Soil: Optimization and Phytotoxicity Study. Toxicology International, 30(1), 83–93. https://doi.org/10.18311/ti/2023/v30i1/31254

Issue

Volume 30, Issue 1, January-March 2023

Section

Articles
Crossref
Scopus
Google Scholar
Europe PMC
Received 2022-09-11
Accepted 2023-01-28
Published 2023-03-20

 

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