Therapeutic Efficacy of Bacopa monnieri against Aflatoxin B1 Induced Toxicity in Rats

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Authors

  • UNESCO Trace Element Satellite Centre, School of Studies in Zoology, Jiwaji University Gwalior - 474001, Madhya Pradesh ,IN
  • UNESCO Trace Element Satellite Centre, School of Studies in Zoology, Jiwaji University Gwalior - 474001, Madhya Pradesh ,IN
  • UNESCO Trace Element Satellite Centre, School of Studies in Zoology, Jiwaji University Gwalior - 474001, Madhya Pradesh ,IN
  • UNESCO Trace Element Satellite Centre, School of Studies in Zoology, Jiwaji University Gwalior - 474001, Madhya Pradesh ,IN
  • UNESCO Trace Element Satellite Centre, School of Studies in Zoology, Jiwaji University Gwalior - 474001, Madhya Pradesh ,IN
  • Department of Zoology, Guru Ghasidas Vishwavidyalaya, Bilaspur - 495009, Chhattisgarh ,IN
  • Brain and Mind Research Institute, Weill Cornell ,US
  • UNESCO Trace Element Satellite Centre, School of Studies in Zoology, Jiwaji University Gwalior - 474001, Madhya Pradesh ,IN
  • UNESCO Trace Element Satellite Centre, School of Studies in Zoology, Jiwaji University Gwalior - 474001, Madhya Pradesh ,IN

DOI:

https://doi.org/10.18311/ti/2024/v31i2/34715

Keywords:

Aflatoxin B1, Bacopa monnieri, Liver, Oxidative Injury, Polyphenol

Abstract

Aflatoxin B1 (AFB1), the most frequently discovered aflatoxin in tainted foods and feed, is considered the most important risk factor. Brahmi (Bacopa monnieri) is a well-known perennial, creeping herb in the Indian Ayurvedic system. Thus, the present study was designed to evaluate the protective efficacy of Brahmi against liver damage induced by Aflatoxin B1. The whole study was conducted in two experiments. The first experiment used phytochemical estimation of BM (Bacopa monnieri). In the second experiment, animals were randomly divided into six groups with six animals in each group. Group 1 served as the control. Group 2 served as per se and received the highest dose of therapy which was 40 mg/kg for 13 days, post orally. Group 3 received AFB1 (200 μg/kg/day) for 13 days, orally Groups 4 to 6 received different doses of Brahmi (20, 30, 40 mg/kg/day PO) for 3 consecutive days after 10 days of exposure to AFB1. All animals were sacrificed after 24 hr of the last treatment. DPPH free radical scavenging activity of the plant was reported in terms of IC50 (45.30 ± 2.52 μg/ml). The presence of flavonoids and protein in the plant was reported in 29.63 ± 1.63 μg Rutin /mg, and 59.72 ± 3.30 μg BSA / mg. Acute studies showed increased lipid peroxidation and a decline in antioxidant status. Alterations in the Liver Function Test (LFT) were also observed. Oral treatment with 20-40 mg/kg Brahmi showed remarkable protection against the toxic effects of Aflatoxin B1. Biochemical results of this study demonstrate that Bacopa monnieri extract possesses protective potential against AFB1-induced hepatotoxicity due to the presence of several bioactive phytochemicals. From this study, we can conclude that treating Bacopa monnieri can protect against AFB1 challenge rats.

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Published

2024-04-15

How to Cite

Rathour, A., Gupte, S. S., Gupta, D., Soni, R., Shrivastava, S., Singh, S., Sharma, A., Yadav, D., & Shukla, S. (2024). Therapeutic Efficacy of <i>Bacopa monnieri</i> against Aflatoxin B<Sub>1</sub> Induced Toxicity in Rats. Toxicology International, 31(2), 239–248. https://doi.org/10.18311/ti/2024/v31i2/34715
Received 2023-08-13
Accepted 2024-03-07
Published 2024-04-15

 

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