Phytochemical Screening and Anti-Diabetic Efficacy of O. Sanctum Seed Extract on Streptozotocin Induced Diabetic Rats


  • Vellalar College for Women, Department of Foods and Nutrition, Erode, Tamilnadu, India


The present study was designed to assess the in vitro and in vivo anti-diabetic efficacy of O. sanctum seed and its phytochemical screening. In vitro inhibitory effect on carbohydrate digestive enzymes like α-amylase and α-glucosidase and in vivo parameters such as fasting blood glucose and body weight changes were studied, a potent inhibitory effect was observed on activities of digestive enzymes and a marked decrease in the glucose level in the O. sanctum seed extract treated streptozotocin induced diabetic rats was noted. Further a marked reduction in body weight was also observed.


Ocimum sanctum seed, anti-diabetic activity, phytochemicals

Subject Discipline

Assistant Professor

Full Text:


Jyothi, S.G., Sahana, C.S., Chavan and Somashekaraiah, B.V. In vitro and in vivo antioxidant and anti-diabetic efficacy of Cassia auriculata L. Flowers. Global J. Pharmacol., 2012, 6, 33–40.

Gin, H. and Rigalleau, V. Post-prandial hyperglycemia and diabetes. Diabetes and Metabol., 2000, 26, 265–272.

Inas, S.G., Ekram, S.A., Hoda, F.B., Ibrahim, M.F. and Somaia, A.N. Evaluation of antihyperglycemic action of oyster mushroom (Pleurotus ostreatus) and its effect on DNA damage, chromosome aberrations and sperm abnormalities in streptozotocin-induced diabetic rats. Global Veterinaria, 2011, 7, 532–544.

Rates, S.M. Plants as source of drugs. Toxicon., 2001, 39, 603–613.

Satyavati, G.V., Gupta, A.K. and Bhatla, N. Ocimum sanctum Linn (Lamiaceae, Labiatea), in medicinal plants of India (Indian Council of Medical Research, New Delhi), 1987, 2, 354.

Chattopadhyay, R.R. Hypoglycaemic effect of Ocimum sanctum leaf extract in normal and streptozotocin diabetic rats, Ind. J. Exp. Biol., 1993, 31, 891.

Sarkar, A. and Pant, M.C. A comparative study of hypoglycaemic action of seeds and fresh leaves of ocimum sanctum (Tulsi), Ind. J. Physiol. Pharmacol., 33, 197.

Singh, S. Comparative evaluation of anti-inflammatory potential of fixed oil of different species of ocimum and its possible mechanism of action, Ind. J. Exp. Biol., 1998, 36, 1028.

Gupta, S.K., Prakash, J. and Srivastava, S. Validation of traditional claim of Tulsi, Ocimum sanctum linn as a medicinal plant, Ind. J. Exp. Biol., 2002, 40, 765.

Pattanayak, P., Behera, P., Das, D. and Panda, S.K. Ocimum sanctum Linn. A reservoir plant for therapeutic applications: An overview, Phcog. Rev., 2010, 4, 95–105.

Alex, B., George, A.K., Johnson, N.B., Patrick, A., Elvis, O.A., Ernest, O.A. et al. Gastroprotective effect and safety assessment of Zanthoxylum Aanthoxyloides (Lam) Waterm root bark extract. Am. J. Pharm.and Toxicol., 2012, 7, 80.

Aboutabl, E.S., Azzam, S.M., Michel, C.G., Selim, N.A. and Saleem, A.A. Lipid and carbohydrates from Ricinus communis L. growing in Egypt and their bioactivities. Bull. Fac. Pharm. Cairo Univ., 2008, 46, 131–139.

Miller, G.L. Use of di-nitro salicylic acid reagent for determination of reducing sugar. Anal. Chem., 1959, 31, 426–428.

Siddique, O., Sun, Y., Lin, J.C. and Chien, Y.W. Facilitated transdermal transport of insulin. J. Pharma. Sci., 1989, 76, 341–345.

Apostolidis, E. and Lee, C. In vitro potential of Ascophyllum nodosum phenolic antioxidantmediated α-glucosidase and α-amylase inhibition, J. Fd. Sci., 2010, 75, 97–102.

Shobana, S., Sreerama, Y.N. and Malleshi, N. Composition and enzyme inhibitory properties of finger millet (Eleusine coracana L.) seed coat phenolics: Mode of inhibition of α-glucosidase and pancreatic amylase, Fd. Chem., 2009, 115, 1268–1273.

Bhat, M., Zinjarde, S.S., Bhargava, S.Y., Kumar, A.R. and Joshi, B.N. Antidiabetic Indian Plants: a Good Source of potent amylase inhibitors. Evidence-Based Complementary and Alternative Med., 2008, 1, 324–328.

UK prospective diabetes study (UKPDS) group Lancet, 1998, 352, 837–853.


  • There are currently no refbacks.