SEM Study on Morphological Changes in Metarhizium anisopliae Infected Aphis craccivora Koch.


Affiliations

  • Assam Agricultural University, Department of Plant Pathology, Jorhat, 785013, India
  • Tezpur University, Department of Environmental Science, Assam, India
  • Assam Agricultural University, Department of Plant Pathology, Jorhat, 785013

Abstract

Morphological changes in Metarhizuim anisopliae (Metschnikoff) Sorokin infected cow pea aphid, Aphis craccivora Koch were studied by scanning electron microscope (SEM). Aphids were caged on paired lantern chimney over cowpea twigs dipped in conical flasks with water infected and inoculated with fungal propagules of M. anisopliae at the concentration of 1X106 spores /ml of water. Infected aphids were observed under SEM and it showed severe cuticular damage, abnormalities in sensory systems as well as deformation of all the body parts. The hydrophobic conidia of M. anisopliae were found to attach to all body regions. It was evident that mycelial growth and conidiophores with conidia of M. anisopliae covered the body surface and penetrates inside the body of infested aphid causing damage to the pest by disturbing its major physiological activities leading to its death.

Keywords

Aphis craccivora, Metarhizuim anisopliae, Scanning Electron Microscope.

Subject Discipline

Botany

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References

Boucias DG, Pendland JC, Latge JP. 1988. Nonspecific factors involved in attachment of entomopathogenic deuteromycetes to host insect cuticle. Appl Environ Microbiol. 54: 1795–1805.

Foster WA. 1975. Life history and population biology of an intertidal aphid, Pemphigus trehernei Foster. Trans R Entomol Soc. 127: 192– 207.

Gabarty A, Salem HM, Fouda MA, Abas AA, Ibrahim AA. 2014. Pathogencity induced by the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in Agrotis ipsilon (Hufn.). J Radiation Res Appl Sci. 7: 95–100.

Moore D, Douro-Kpindou OK, Jenkins NE, Lomer CJ. 1996. Effect of moisture content and temperature on storage of Metarhizium flavoviridae conidia. Biocontl Sci Technol. 6: 51–61.

Moino A, Alves SB, Lopes RB, Oliveira PM, Neves J, Pereira RM, Vieira SA. 2002. External development of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in the subterranean termite Heterotermes tenuis. Scientia Agricola. 59(2): 267–273.

Neves PMOJ, Alves SB, 2004. External events related to the infection process of Cornitermes cumulans (Kollar) (Isoptera: Termitidae) by the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae. Neotropl Entomol. 33:51–56.

Pedrini N, Crespo R, Juarez MP. 2007. Biochemistry of insect epicuticle degradation by entomopathogenic fungi. Comp Biochem Physiol. 146: 124–137.

Pegu JR, Dutta P, Puzari KC, Nath PD, Das P. 2012. First report of Metarhizium anisopliae (Metchinkoff) Sorokin on cow pea aphid (Aphis craccivora Koch) (Homoptera: Aphididae) from North East India. J Mycol Pl Pathol. 42(1): 174–175.

Puzari KC, Hazarika LK, Deka N. 1994. Pathogenicity of Beauveria bassiana (Bals.) Vuill on developmental stages of rice hispa, J Biol Control 8(2): 133–135.

Schneider LCL, Silva CV, Pamphile JA, Conte H. 2013. Infection, colonization and extrusion of Metarhizium anisopliae (Metsch) Sorokin (Deuteromycotina: Hyphomycetes) in pupae of Diatraea saccharalis F. (Lepidoptera: Crambidae). J Entomol Nematol. 5(1): 1–9.

St Leger RJ. 1991. Integument as a barrier to microbial infections. pp. 284–306. In: Binnington K, Retnakaran A. (Eds), Physiology of the insect epidermis. CSIRO, Melbourne, Australia.

Toledo AV, de Remes Lenicov AMM, Lopez Lastra CC. 2010. Histopathology caused by the entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae, in the adult planthopper, Peregrinus maidis, a Maize Virus Vector. J Ins Sci. 10: 35.

Vestergaard S, Butt TM, Bresciani J, Gillespie AT, Eilenberg J. 1999. Light and electron microscopy studies of the infection of the Western flower thrips Frankliniella occidentalis (Thysanoptera: Thripidae) by the entomopathogenic fungus Metarhizium anisopliae. J Inv Pathol. 73: 25–33.

Zhang X, Yang Y, Zhang L. 2010. Ultrastructure of the entomopathogenic fungus Metarhizium anisopliae during cuticle penetration in the locust, Locusta migratoria (Insecta: Acrididae). J Ortho Res. 19(1): 115–119.


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