Combined effect of Eichhornia crassipes and Emblica officinalis on serum electrolytes and enzyme profile after arsenic intoxication in albino rats




Enzyme profile, Serum electrolytes, Albino rat, Arsenic water, Eichhornia crassipes, Emblica officinalis


The present investigation was carried out to evaluate the combined effect of Eichhornia crassipes and Emblica officinalis on serum electrolytes and enzyme profile after arsenic intoxication in albino rats. E. officinalis has been used as a valuable ingredient of various medicines in India. Recent investigations has given ample evidences of various uses of Eichhornia crassipes as hyper accumulator of various toxins. Three groups of ten adult male albino rats each were administered 0.102 mgA. arsenic present in drinking water (collected from Sikandara area of Agra) for 30, 45 and 60 days and another three groups of ten rats were treated first with arsenic water in the same way as mentioned above and then treated with combined E. crassipes (lOgm/Kg body weight) and E. officinalis (500 mg/Kg body weight) fruit extract (mixed in ratio of 1:2) orally for 15, 30 and 45 days, respectively. Results showed a very highly significant (p<0.001) increase in arsenic in the seaim, a very highly significant (p<0.001) decrease in calcium, magnesium, sodium, significant (p< 0.05) decrease in potassium, very highly significant (p<0.001) increase in serum AST, ALT and nonsignificant (p>0.05) increase in LDH after arsenic water intoxication; while a very highly significant (p<0.001) decrease in serum arsenic, a very highly significant (p<0.001) increase in calcium, potassium, a non-significant increase in magnesium, non-significant (p>0.05) decrease in sodium, a very highly significant (p<0.001) decrease in serum AST, ALT and non-significant (p>0.05) decrease in LDH after combined E. crassipes and E. officinalis treatment, respectively E. crassipes reduces the serum arsenic burden through chelation biomechanism and provides nutrients; while E. officinalis acts as a strong antioxidant We concluded that E. crassipes and £ officinalis have compensated the arsenic toxicity that affects the body metabolism


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Author Biographies

Seema Chaudhary

Department of Zoology, School of Life Sciences, Dr. B.R. Ambedkar University, Khandari Campus, Agra-282002, India

P.K. Singh

Department of Zoology, School of Life Sciences, Dr. B.R. Ambedkar University, Khandari Campus, Agra-282002, India

Anil Kr. Gupta

Department of Zoology, School of Life Sciences, Dr. B.R. Ambedkar University, Khandari Campus, Agra-282002, India

Ajay Pratap Singh

Department of Zoology, School of Life Sciences, Dr. B.R. Ambedkar University, Khandari Campus, Agra-282002, India


Achliya, G.S., Wadodkar, S.G. and Dorle, A.K. (2004) Evaluation of hepatoprotective effect of Amalkadi Ghrita against carbon tetrachioride-induced hepatic damage in rats. J. Ethnopharmacol., 90, 229-232.

Aggett, J. and Aspell, A.C. (1976) Determination of arsenic (III) and total arsenic by the silver diethyldithiocarbamate method. Analyst, 101, 912-915.

Bhaftacharya, S.K., Bhattacharya, A., Sairam, K. and Ghosal, S. (2002) Effect of bioactive tannoid principles of Emblica officinalis on ischemiareperfusioninduced oxidative stress in rat heart. Phytomed., 9, 171-174.

Biswas, U., Sarkar, S., Bhowmik, M. K., Samanta, A. K. and Biswas, S. (2000) Chronic toxicity of arsenic in goats: Ciinicobiochernical changes, pathomorphology and tissue residues. Small Ruminant Res., 38, 229-235.

Campillo, N., Vinas, P., Garcia, i.L. and Cordoba, M.H. (2000) Determination of arsenic in biological fluids by electrothermal atomic absorption spectrometry. Analyst, 125, 313-316.

Chowdhury, T.R., Mandal, B.K. and Samanta, G. (1997) Arsenic in groundwater in six districts of West Bengal, India: the biggest arsenic calamity in the world: A status report upto August, 1995. Arsenic Exposure and health effects, Eds. CO. Abernathy, R.L.Calderon, W.R. Chappell. Chapman and Hall, London, 9, 93-111.

Flora, S.J., Mittal, M. and Mehta, A. (2008) Heavy metal induced oxidative stress and its possible reversal by chelation therapy. Ind. J. Med. Res., 128, 501523.

Islam, L. N., Nabi, A.H.M.N., Rahman, M.M., Khan M.A. and Kazi, A.I. (2004) Association of clinical complications with nutritional status and the prevalence of leukopenia among arsenic patients in Bangladesh. Int. J. Environ. Res. Pub. HIth., 1, 74-82.

Mathur, R., Sharma, A., Dixit, V.P. and Varma, M. (1996) Hypolipidaemic effect of fruit juice of Emblica officinalis in cholesterol-fed rabbits. J. Ethnophanriacol., 50, 61-68.

Misbahuddin , M., Islam, A.Z.M.M., Khandker, S., Mahmud, I.A., Islam, N. and Anjumanara (2006) Efficacy of spirulina extract plus zinc in patients of chronic poisoning: A randomized placebocontrolled study. Clin. Toxicol., 44,135-141.

Misbahuddin, M. and Fariduddin, A. (2002) Water Hyacinth removes arsenic from arseniccontaminated drinking Water. Arch. Environ. HIth.,57, 516-518.

Mittal, M. and Flora, S.J. (2006) Effects of individual and combined exposure to sodium arsenite and sodium fluoride on tissue oxidative stress, arsenic and fluoride levels in male mice. Chem. Boil. Interact., 162, 128-139.

Modi, M., Mittal, M. and Flora, S.J.S. (2007) Combined administration of selenium and meso-2, 3dimercaptosuccinic acid on arsenic mobilization and tissue oxidative stress in chronic arsnicexposed male rats. Ind.J.Pharmacol., 39, 107-114.

Modi, M., Pathak, U., Kalia, K. and Flora, S.J.S. (2005) Arsenic antagonism studies with monoisoamyl DMSA and zinc in male mice. Environ. Toxicol. Pharmacol., 19, 131-138.

Mukherjee, S., Das, D., Mukherjee, M., Das, A.S. and Mitra, C. (2006) Synergistic effect of folic acid and vitamin B12 in ameliorating arsenic-induced oxidative damage in pancreatic tissue of rat. Food Chem. Toxicol., 17, 319-327.

Nandi, D., Patra, R. C. and Swarup, D. (2005) Effect of cysteine, methionine, ascorbic acid and thiamine on arsenic induced oxidative stress and biochemical alterations in rats. Toxicol., 211, 2635.

Nandi, D., Patra, R. C. and Swarup, D. (2006) Oxidative stress indices and plasma biochemical parameters during oral exposure to arsenic in rats. Food Chem. Toxicol., 44, 1579-1584.

Nandi, D., Patra, R.C., Ranjan, R. and Swarup, D. (2008) Role of co-administration in prevention of oxidative injury following sub-chronic exposure to arsenic in rats. Vet. Archiv, 78 , 113-121.

Oba, PC. and Uriteni P. (1982) Recommendation for the measurement of LDH in serum at 3000C. Ann.Biol. Chem., 40, 87-89.

Pal, S. and Chatterjee, A.K. (2004) Protective effect of methionine supplementation on arsenic-induced alteration of glucose homeostasis. Food Chem. Toxicol., 42, 737-742.

Peraza, M.A., Carter, D.E. and Gandolfi, A.J. (2003) Toxicity and metabolism of subcytotoxic inorganic arsenic in human renal proximal tubule epithelial cells (HK-2). Cell Biol, and Toxicol., 19, 253-264.

Piper, C. S. (1950) In: Soil and Plant Analysis, Interscience Publishers, Inc., New York, pp. 279281.

Quayum, S.L, (2007) Effect of water hyacinth root extract on arsenic level in different organs of arsenictreated rat. Bangladesh J.Phanvacol., 2, 73-80.

Qureshi, S.A., Asad, W. and Sultana, V. (2009) The effect of Phyllantus emblica Linn on type - II diabetes, triglycerides and liver - specific enzyme. Pakistan J. Nutr., 8, 125-128.

Rana, T, Sarkar, S., Mandal, T and Batabyal, S. (2008) Haematobiochemical profiles of affected cattle at arsenic prone zone in Haringhata block of Nadia district of West Bengal in India. The Internet J.Haematol.. 4. 15-19.

Rao, T.P., Sakaguchi, N., Juneja, L.R., Wada, E. and Yokozawa, T. (2005) Amia {EmbUea officinalis Gaertn.) extracts reduce oxidative stress in streptozotocin-induced diabetic rats. J. Med. Food, 8, 362-368.

Rehman, H.U., Yasin, K.A. and Choudhary, M.A. (2007) Studies on the chemical constituents of Phyllanthus emblica. Nat. Prod. Res., 21, 775781.

Rehman, M. M., Choudhary, U.K. and Mukherjee, S.C.(2002). Chronic arsenic toxicity in Bangladesh and West Bengal, India. Review and commentary. J. Toxicol. Clin. Toxicol., 40, 527-530.

Reitman, S. and Frankel, S. (1957) Estimation of SCOT and SGPT in serum. Am. J. Clin. Path., 28, 5662.

Rmalli, W.S.A., Hamngton, C.F., Ayub, M. and Haris, Rl. (2005) A biomaterial based approach for arsenic removal from water. J. Environ. Monit., 7,279-282.

Saeed, S. and Tariq, P. (2007) Antibacterial activities of Emblica officinalis and Coriandrum sativum against Gram negative urinary pathogens. Pak. J. Pharm. Sci., 20, 32-35.

Schoolmeester, W. L. and White, D.R. (1980) Arsenic poisoning. South. Med. J., 73, 198-208.

Thangaraj, R., Ayyappan, S.R., Manikandan, P. and Baskaran, J. (2007) Antioxidant property of Emblica officinalis during experimentally induced restrain stress in rats. J.HIth.Sci., 53, 496-499.

Trinder, P., (1960) Estimation of calcium ion concentration in the serum. Analyst, 85, 889.

Umar, B.U., (2007) Effect of hexane extract of spinach in the removal of arsenic from rat. Bangladesh J. Phanvacol.. 2, 27-34.

Upadhyay, A.R. and Tripathi, B.D. (2007) Principle and process of biofiltration of Cd, Cr, Co, Ni and Pb from tropical opencast coalmine effluent. Water, Air, and Soil Poll., 180, 213-223.

Varley, H., Gowenlock, A. H. and Bell, M. (1980) Practical Biochemistry, 5th ed., William Heniemann Medical Books, Ltd., London, pp 18-21.

Wang, L., Xu, Z., Jia, X. and Han, X. (2006) Effects of dietary arsenic levels on serum parameters and trace mineral retentions in growing and finishing pigs. Biol. Trace Element Res., 113, 155-164.




How to Cite

Chaudhary, S., Singh, P., Gupta, A. K., & Pratap Singh, A. (2017). Combined effect of Eichhornia crassipes and Emblica officinalis on serum electrolytes and enzyme profile after arsenic intoxication in albino rats. Journal of Ecophysiology and Occupational Health, 10(3-4), 111–119.