Behavioural Response of Specific Larval Endoparasitoid, Apanteles machaeralis (Wilkinson) to Volatile Cues from its Host Insect, Diaphania indica (Saunders) and the Host Plant (Cucumis sativus L.)

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Authors

  • U. Venugopal
     Department of Entomology, University of Agricultural Sciences, Bangalore – 560065, Karnataka ,IN
  • P. D. Kamala Jayanthi
     Division of Entomology and Nematology, ICAR-Indian Institute of Horticultural Research, Hessaraghatta Lake PO, Bangalore – 560089, Karnataka ,IN
  • P. Saravan Kumar
     Division of Entomology and Nematology, ICAR-Indian Institute of Horticultural Research, Hessaraghatta Lake PO, Bangalore – 560089, Karnataka ,IN
  • K. S. Jagadeesh
     Department of Entomology, University of Agricultural Sciences, Bangalore – 560065, Karnataka ,IN
  • K. Murali Mohan
     Department of Entomology, University of Agricultural Sciences, Bangalore – 560065, Karnataka ,IN

DOI:

https://doi.org/10.18311/jbc/2020/25359

Keywords:

Cucumber Moth, HIPVs, Olfactometer Assays, Specialist Parasitoid, Volatiles.

Abstract

Apanteles machaeralis (Wilkinson), a braconid specialist larval endoparasitoid of Diaphania indica occurs naturally causing significant levels of parasitism.  The present study explores the response of the A. machaeralis to odour cues from D. indica damaged cucumber plant as well as host larvae. Different odour treatments namely, D. indica larval body volatiles (T1), volatiles from larval excreta (T2), volatiles from larvae + excreta (T3),  volatiles from D. indica body wash (T4), volatiles from the healthy, mechanically damaged and D. indica infested cucumber plants (HIPVs) were collected using headspace analysis and the response of female A. machaeralis was studied using olfactometer assays. Results of the study conclusively indicated that A. machaeralis is highly attracted to host larval body wash as parasitic wasps spent significantly more time made more entries into the treated region in single as well as dual choice assays. The electoantennographic response (EAG) further supported the olfactometer bioassays.  The GC-MS analysis revealed significant differences in the volatile emissions of different treatments studied. The utilization of host insect body cues and HIPVs in the host recognition by the specialist endoparasitoid A. machaeralis is discussed in detail.

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Published

2020-10-20

How to Cite

Venugopal, U., Kamala Jayanthi, P. D., Saravan Kumar, P., Jagadeesh, K. S., & Murali Mohan, K. (2020). Behavioural Response of Specific Larval Endoparasitoid, <I>Apanteles machaeralis</I> (Wilkinson) to Volatile Cues from its Host Insect, <I>Diaphania indica</I> (Saunders) and the Host Plant (<I>Cucumis sativus</I> L.). Journal of Biological Control, 34(2), 132–139. https://doi.org/10.18311/jbc/2020/25359

Issue

Volume 34, Issue 2, June 2020

Section

Research Articles
Crossref
Scopus
Google Scholar
Europe PMC
Received 2020-05-18
Accepted 2020-09-14
Published 2020-10-20

 

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