Natural occurrence of entomopathogenic fungus, <i>Aschersonia aleyrodis</i> on citrus whitefly, <i>Dialeurodes citri</i> (Ashmead) in Kinnow mandarin in Punjab, India

Authors

  • Sandeep Singh Department of Fruit Science, Punjab Agricultural University, Ludhiana – 141004, Punjab
  • Bonam Ramanujam ICAR-National Bureau of Agricultural Insect Resources (NBAIR), Bengaluru – 560024, Karnataka
  • Rajwinder Kaur Sandhu ICAR-National Bureau of Agricultural Insect Resources (NBAIR), Bengaluru – 560024, Karnataka
  • A. Kandan ICAR-National Bureau of Agricultural Insect Resources (NBAIR), Bengaluru – 560024, Karnataka
  • B. Poornesha ICAR-National Bureau of Agricultural Insect Resources (NBAIR), Bengaluru – 560024, Karnataka
  • Rupa Kundu ICAR-National Bureau of Agricultural Insect Resources (NBAIR), Bengaluru – 560024, Karnataka

DOI:

https://doi.org/10.18311/jbc/2021/29506

Keywords:

<i>Aschersonia aleyrodis</i>, biological control, citrus whitefly, entomopathogenic fungi, Punjab

Abstract

Surveys were conducted during 2017 and 2018 in the citrus orchards of Punjab, India to record the incidence of different insect pests and their natural enemies. During October-December, Entomopathogenic Fungus (EPF), Aschersonia aleyrodis was found to infect nymphs and pupae of citrus whitefly, Dialeurodes citri on the lower leaf surface of Kinnow from the orchards of Hoshiarpur, Ludhiana, Mansa and Fazilka districts.The fungus was isolated from the infected nymphs and pupae and morphological studies were conducted to confirm the identity of the entomopathogenic fungus. Aschersonia aleyrodis was reported for the first time on D. citri under Punjab conditions and this EPF also confirmed by amplification and sequencing of beta tubulin gene showed 99.40 per cent identity in NCBI, GenBank. Hence further studies on the host range, interaction with other insect pests and parasitoids, survival and longevity should be conducted to explore the potential of this fungus as microbial biocontrol agent for citrus whitefly.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. 1990. Basic local alignment search tool. J Mol Biol. 215: 403– 41. https://doi.org/10.1016/S0022-2836(05)80360-2

Anonymous. 2018. Horticultural statistics at a glance 2018. Government of India. 490 pp.

Anonymous. 2019. Package of Practices for Cultivation of Fruits. Punjab Agricultural University, Ludhiana, 187 pp.

Butt TM. 1990. Fungal infection processes - A mini review. Proceedings and abstracts, 5th International Colloquium on Invertebrate Pathology and Microbial Control, Adelaide, Australia, pp. 121–4.

Bischoff JF, Rehner SA, Humber RA. 2009. A multilocus phylogeny of the Metarhizium anisopliae lineage. Mycologia. 101: 512–30. PMid: 19623931. https://doi.org/10.3852/07-202

Clustal W and Clustal X version 2.0. Bioinform. 23: 2947–8. PMid: 17846036. https://doi.org/10.1093/bioinformatics/btm404

Debnath S. 2016. Studies on entomopathogenic fungus Aschersonia aleyrodis infectious to scale insect pest, Saisettia formicarii of tea, Camellia sinensis (L.). O. Kuntze in Assam. Indian J Fundamental Appl Life Sci. 6(3): 14–24.

Dutta P, Pegu J, Puzari KC. 2013. Current status and future prospects of entomopathogenic fungi in North East India. KAVAKA. 41: 75–86.

Fasulo TR, Weems HV. 2017. Citrus whitefly, Dialeurodes citri (Ashmead) (Insects: Hemiptera: Aleyrodidae). http:// entnemdept.ufl.edu/creatures/citrus/citrus_whitefly.htm Flint ML. 2015. Whiteflies Integrated Pest Management for Homes, Gardens and Landscape. PEST NOTES Publication 7401.

Fransen JJ. 1990. Natural enemies of whiteflies: Fungi. In: Gerling D, ed. Whiteflies: Their Bionomics, Pest status and Management. Intercept Ltd, Andover, UK, pp. 187– 210.

Fransen JJ, Lenteren JC. 1993. Host selection and survival of the parasitoid Encarsia formosa on greenhouse whitefly, Trialeurodes vaporariorum, in the presence of hosts infected with the fungus Aschersonia aleyrodis. Entomol. Exp. Appl. 69: 239–49. https://doi.org/10.1111/j.1570-7458.1993.tb01747.x

Fransen JJ, Lenteren JC. 1994. Survival of the parasitoid Encarsia formosa after treatment of parasitized greenhouse whitefly larvae with fungal spores of Aschersonia aleyrodis. Entomol. Exp. Appl. 71: 235–43. https://doi.org/10.1111/j.1570-7458.1994.tb01790.x

Felsenstein J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution. 39: 783–91. PMid: 28561359. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x

Gao RX, Ouyang Z, Gao ZX, Zheng JX. 1985. A preliminary report on citrus whitefly control with Aschersonia aleyrodis. J Fujian Agric College. 14(2): 127–33.

Kumar S, Stecher G, Li M, Knyaz C, Tamura K. 2018. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Mol. Biol. Evol. 35: 1547– 9. PMid: 29722887 PMCid: PMC5967553. https://doi.org/10.1093/molbev/msy096

Lacey LA, Fransen JJ, Carruthers R. 1996. Global distribution of naturally occurring fungi ofBemisia, their biologies and use as biological control agents. Gerling D, Mayer R, eds. Bemisia: Taxonomy, Biology, Damage and Management. Intercept Ltd, Andover, UK. pp. 401–33.

Lacey LA, Wraight S, Kirls A. 2003. Entomopathogenic fungi for control of Bemisia spp.: Foreign exploration and implementation. USDA Miscellaneous publication. pp. 1343.

Liu M, Chaverri P, Hodge KT. 2006. A taxonomic revision of the insect biocontrol fungus Aschersonia aleyrodis, its allies with white stromata and their hypocrella sexual states. Myco Res. 110: 537–54. PMid: 16769508. https://doi.org/10.1016/j.mycres.2006.01.013

McCoy CW, Samson RA, Boucias, DG. 1988. Entomogenous fungi. Ignoffo CM, Mandava NB, eds. Entomogenous protozoa and fungi. pp. 151–236. https://doi.org/10.1201/9780429487187-2

Meekes ETM, Fransen JJ, Lenteren JC. 1994. The use of entomopathogenic fungi for the control of whiteflies. Mededelingen van de Faculteit der Landbouwwetenschappen Rijksuniversiteit Gent. 59(2a): 371–7.

Meekes ETM, Fransen JJ, Lenteren JC. 2002. Pathogenicity of Aschersonia spp. against whiteflies Bemisia argentifolii and Trialeurodes vaporariorum. J Invertebrate Path. 81(1): 1–11. https://doi.org/10.1016/S00222011(02)00150-7

Meekes ETM, Voorst S, Joosten NN, Fransen JJ, Lenteren JC. 2000. Persistence of the fungal whitefly pathogen, Aschersonia aleyrodis, on three different plant species. Myco Res. 104(10): 1234–40. https://doi.org/10.1017/S0953756200002513

Osborne LS, Landa Z. 1992. Biological control of whiteflies with entomopathogenic fungi. The Flo Entomol. 75(4): 456–71. https://doi.org/10.2307/3496127

Qiu J, Song F, Mao L, Tu J, Guan X. 2013. Time-dosemortality data and modeling for the entomopathogenic fungus Aschersonia placenta against the whitefly Bemisia tabaci. Canadian J Micro. 59(2): 97–101.

PMid: 23461516. https://doi.org/10.1139/cjm-2012-0569

Saitou N, Nei M. 1987. The Neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406–25.

Sudiarta IP, Suputra IPW, Wirya GNAS. 2019. New report of insect pathogenic fungi (Aschersonia sp.) of citrus whitefly (Dialeurodes sp.) in Bali Indonesia. Agric Vet Sci. 3(1): 22–7.

Wang P, Song X, Zhang H. 2013. Isolation and characterization of Aschersonia placenta from citrus orchards and its pathogenicity towards Dialeurodes citri (Ashmead). J Invertebrate Path. 112: 122–8. PMid: 23160084. https://doi.org/10.1016/j.jip.2012.10.005

Weisskopf A, Fuller DQ. 2014. Citrus Fruits: Origins and Development. Smith C, ed. Encyclopedia of Global Archaeology. New York: Springer; https://doi.org/10.1007/978-1-4419-0465-2_2173

Zhang C, Ali S, Musa PD, Wang XM, Qiu BL. 2017. Evaluation of the pathogenicity of Aschersonia aleyrodis on Bemisia tabaci in the laboratory and greenhouse. Biocontrol Sci Tech. 27(2): 210–21. https://doi.org/10.1080/09583157.2016.1274878

Downloads

Published

2022-06-05

How to Cite

Singh, S., Ramanujam, B., Kaur Sandhu, R., Kandan, A., Poornesha, B., & Kundu, R. (2022). Natural occurrence of entomopathogenic fungus, <i>Aschersonia aleyrodis</i> on citrus whitefly, <i>Dialeurodes citri</i> (Ashmead) in Kinnow mandarin in Punjab, India. Journal of Biological Control, 35(3), 181–186. https://doi.org/10.18311/jbc/2021/29506

Issue

Section

Research Articles

Most read articles by the same author(s)