In vitro evaluation of microencapsulated Bacillus thuringiensis (Berliner) formulation against Helicoverpa armigera (Hubner)

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Authors

  • K. AKSHAYA KUMAR
     Department of Agricultural Entomology, College of Agriculture, Raichur – 584104, Karnataka ,IN
  • BASAVARAJ S. KALMATH
     Department of Agricultural Entomology, College of Agriculture, Raichur – 584104, Karnataka ,IN
  • B. KISAN
     Department of Biotechnology, MARS, Raichur – 584104, Karnataka ,IN
  • A. PRABHURAJ
     Pesticide Residue and Food Quality Analysis Laboratory (PRFQAL), MARS, Raichur – 584104, Karnataka ,IN
  • S. MALLIKARJUNA
     Department of Agricultural Entomology, College of Agriculture, Bheemarayanagudi - 585223, Karnataka ,IN
  • M. BHEEMANNA
     Pesticide Residue and Food Quality Analysis Laboratory (PRFQAL), MARS, Raichur – 584104, Karnataka ,IN

DOI:

https://doi.org/10.18311/jbc/2024/35665

Keywords:

Bacillus thuringiensis, bioassay, Helicoverpa armigera, microencapsulation, PCR, UV protectants

Abstract

An experiment was conducted to evaluate microencapsulated formulation of  lyophilized spore crystal aggregate of native isolate BGC-1 and reference isolate HD-1 against second instar larvae of Helicoverpa armigera. The results revealed that the microcapsule diameter was ranged from 3.2 to 8.3 µm. Median lethal concentrations of the BGC-1 and Bt-HD1 were 0.66 g/l and 0.50 g/l respectively. UV protectants viz., melanin and para-amino benzoic acid were evaluated by exposing microencapsulated Bacillus thuringiensis to UV A light at 365nm. Among four microencapsulated formulations, BGC-1 with melanin recorded significantly highest mortality of 95.00 per cent at 0h exposure, as time increased, the mortality decreased and HD-1 was on par with BGC-1.

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Published

2024-04-11

How to Cite

K. AKSHAYA KUMAR, KALMATH, B. S., KISAN, B., PRABHURAJ, A., MALLIKARJUNA, S., & BHEEMANNA, M. (2024). <i>In vitro</i> evaluation of microencapsulated <i>Bacillus thuringiensis</i> (Berliner) formulation against <i>Helicoverpa armigera</i> (Hubner). Journal of Biological Control, 38(1), 41–50. https://doi.org/10.18311/jbc/2024/35665

Issue

Volume 38, Issue 1, March 2024

Section

Research Articles

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Copyright (c) 2024 K. AKSHAYA KUMAR, BASAVARAJ S. KALMATH, B. KISAN, A. PRABHURAJ, S. MALLIKARJUNA, M. BHEEMANNA (Author)

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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Received 2023-11-21
Accepted 2024-01-08
Published 2024-04-11

 

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