Evaluation of Two Species of Entomopathogenic Fungi against White Grub, Holotrichia consanguinea (Blanchard) Infesting Potato in Maharashtra, India
Keywords:Beauveria bassiana, Holotrichia consanguinea, Metarhizium anisopliae and Potato.
AbstractThe efficacy of two entomopathogenic fungi, Metarhizium anisopliae and Beauveria bassiana, against white grub, Holotrichia consanguinea infesting potato crop was studied under laboratory and field conditions. The estimated LC50 of M. anisopliae and B. bassiana towards third instar larvae of H. consanguinea were 5.76 x 105 and 7.50 x 105 conidia ml-1, respectively. The LT50 of M. anisopliae and B. bassiana at the concentration of 8 x 105 conidia ml-1 against third instar larvae of H. consanguinea were 4.88 and 6.73 days, respectively. In field experiments, M. anisopliae @ 2 x 1012 conidia ha-1 was found to be effective with an average efficacy of 46.74%, while it was 41.32% in phorate 10G @ 25kg ha-1. The maximum mycosis was recorded with the use of M. anisopliae (44.44%). The tuber infestation was statistically lowest (6.91%) and yield of marketable tubers highest (27.6t ha-1) with the use of M. anisopliae as against 27.85% and 17.8t ha-1 in untreated plot, respectively.
Abbott, W. S. 1925. A method for computing the effectiveness of an insecticide. Journal of Economic Entomology, 18: 265-267.
Ansari, M. A., Vestergaard, S., Tirry, L. and Moens, M. 2004. Selection of a highly virulent fungal isolate, Metarhizium anisopliae CLO 53, for controlling Hoplia philanthus. Journal of Invertebrate Pathology, 85: 89-96.
Butt, T. M., Ibrahim, L., Clark, S. J. and Beckett, A. 1997. The germination behaviour of Metarhizium anisopliae on the surface of aphid and Xea beetle cuticles. Mycological Research, 99: 945-950.
Beron, C. M. and Diaz, B. M. 2005. Pathogenicity of hypomycetous fungi against Cyclocephala signaticollis. Biocontrol, 50: 143-150.
Chandla, V. K. 1985. Potato pests and their management. Indian Farming, 34: 31-32.
Desai, M. T. and Patel, R. M. 1965. Some observations on the biology and control of white grub in soil (Holotrichia consanguinea Blanch.) affecting groundnut and cereals in Gujarat. Indian Journal of Entomology, 27: 89-94.
Finney, D. J. 1964. Probit Analysis. A statistical treatment of the sigmoid response curve. 2nd ed., Cambridge University Press, London, 318 pp.
Gomez, K. A. and Gomez, A. A. 1984. Statistical Procedures for Agricultural Research. 2nd Edn., John Wiley and Sons, New York, 680 pp.