Potency of Clitoria ternatea L. Ethanol Extract Against IFNγ and GLUT4 Protein in Type 2 Diabetes Mellitus Rat Model

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Authors

  • Philips Onggowidjaja
     Faculty of Medicine, Maranatha Christian University, Bandung - 40163, West Java ,ID
  • Rita Tjokropranoto
     Faculty of Medicine, Maranatha Christian University, Bandung - 40163, West Java ,ID
  • Richard Gunawan
     Faculty of Medicine, Maranatha Christian University, Bandung - 40163, West Java ,ID
  • Wahyu Widowati
     Faculty of Medicine, Maranatha Christian University, Bandung - 40163, West Java ,ID
  • Hanna Sari Widya Kusuma
     Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung - 40163, West Java ,ID
  • Faradhina Salfa Nindya
     Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung - 40163, West Java ,ID

DOI:

https://doi.org/10.18311/jnr/2024/32205

Keywords:

Clitoria ternatea, Diabetes Mellitus, IFNγ, Immunohistochemistry, GLUT4

Abstract

Diabetes Mellitus (DM) is a chronic disease caused by genetics, lifestyle, and immunology. Type 2 DM is caused by resistanceto insulin, which causes blood sugar to not be stored in glycogen. Clitoria ternatea L. (Fabaceae) flower contains a lot ofanthocyanins and has long been used in various traditional medicines. They are believed to treat fever, inflammation, anddiabetes. Anthocyanins have antidiabetic, anticancer, anti-inflammatory, and anticancer properties. This study aimed toevaluate anti-DM of Clitoria ternatea L. flower extract (CTE) in DM model rats with parameters such as liver weight ratio,IFNγ, and GLUT4 protein expression in pancreas and femoral muscle. Rats with a streptozotocin-induced (STZ) DM modelwere used to test the effects of oral administration of CTE at doses of 200, 400, and 800 mg/kg BW, glybenclamide (0.45mg/kg BW), and simvastatin (0.9 mg/kg BW). After blood sugar levels were >200 mg/dL, the rats were given various dosesof CTE and tested for protein expression of IFNγ and GLUT4 in femoral muscle by immunohistochemistry (IHC) method.Data analysis used ANOVA and continued with Tukey’s post hoc test. Results showed that CTE could increase liver ratiowhile decreasing IFNγ activation at CTE 400 mg/kg BW and upregulating GLUT4 at 400 mg/kg BW. CTE has an anti-DM activity potential for diabetes mellitus treatment due to its anthocyanin content.

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Published

2024-01-01

How to Cite

Onggowidjaja, P., Tjokropranoto, R., Gunawan, R., Widowati, W., Widya Kusuma, H. S., & Nindya, F. S. (2024). Potency of <i>Clitoria ternatea</i> L. Ethanol Extract Against IFNγ and GLUT4 Protein in Type 2 Diabetes Mellitus Rat Model. Journal of Natural Remedies, 24(1), 147–154. https://doi.org/10.18311/jnr/2024/32205

Issue

Volume 24, Issue 1, January 2024

Section

Research Articles

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Copyright (c) 2024 Philips Onggowidjaja, Rita Tjokropranoto, Richard Gunawan, Wahyu Widowati, Hanna Sari Widya Kusuma, Faradhina Salfa Nindya (Author)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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Europe PMC
Received 2022-12-20
Accepted 2023-10-17
Published 2024-01-01

 

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