Identification and Quantification of Kaempferol From Ethyl Acetate Fraction of Pluchea wallichiana DC Leaves

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Authors

  • Kinjal Bera
     Department of Pharmacognosy, Parul Institute of Pharmacy, Parul University, Limda, Vadodara - 391760, Gujarat ,IN
  • Madhavi Patel
     Department of Pharmacognosy, Parul Institute of Pharmacy, Parul University, Limda, Vadodara - 391760, Gujarat ,IN

DOI:

https://doi.org/10.18311/jnr/2023/33208

Keywords:

Ethyl Acetate Fraction, Kaempferol, P. wallichiana DC Leaves

Abstract

P. wallichiana DC, commonly known as camphor weed, is practically used in treatment of inflammatory disorders. Identification of kaempferol was done using High-Resolution Liquid Chromatography-Mass Spectrometry (HRLC-MS). High-Performance Thin-Layer Chromatography (HPTLC) method was performed and validated for the quantification of kaempferol. With a correlation coefficient of 0.997, the linearity range for kaempferol was 200 to 700 ng/spot. The suggested method had a recovery rate between 99.60 and 99.85 %. Limit of detection and Limit of quantification were determined to be 25 and 76.47 ng per spot, respectively. The amount of kaempferol was found to be 7.2% in ethyl acetate fraction of leaves. This is the first report of identification and quantification of kaempferol from P. wallichiana DC through HRLC-MS and HPTLC methods.

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Published

2023-08-31

How to Cite

Bera, K., & Patel, M. (2023). Identification and Quantification of Kaempferol From Ethyl Acetate Fraction of <i>Pluchea wallichiana</i> DC Leaves. Journal of Natural Remedies, 23(3), 999–1008. https://doi.org/10.18311/jnr/2023/33208

Issue

Volume 23 Issue 3 July 2023

Section

Research Articles

License

Copyright (c) 2023 Kinjal Bera, Madhavi Patel (Author)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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Europe PMC
Received 2023-03-01
Accepted 2023-06-26
Published 2023-08-31

 

References

Saggar S, Mir PA, Kumar N, Chawla A, Uppal J, Kaur A. Traditional and herbal medicines: opportunities and challenges. Pharmacogn Res. 2022; 14(2):107-14. https:// doi.org/10.5530/pres.14.2.15 DOI: https://doi.org/10.5530/pres.14.2.15

Kumari R, Kotecha M. A review on the standardization of herbal medicines. International Journal of Pharma Sciences and Research. 2016; 7(2):97-106.

Chothani DL, Patel MB, Mishra SH. HPTLC-fingerprint profile and isolation of marker compound of Ruellia tuberosa. Chrom Res Int. 2012; 2012:1-6. https://doi. org/10.1155/2012/180103 DOI: https://doi.org/10.1155/2012/180103

Patil PS, Shettigar R. An advancement of analytical techniques in herbal research. Journal of Advanced Scientific Research. 2010; 1(01):8-14.

Liang Y-Z, Xie P, Chan K. Quality control of herbal medicines. Journal of chromatography B. 2004; 812(1- 2):53-70. https://doi.org/10.1016/S1570-0232(04)00676-2 DOI: https://doi.org/10.1016/S1570-0232(04)00676-2

Ong ES. Chemical assay of glycyrrhizin in medicinal plants by Pressurized Liquid Extraction (PLE) with Capillary Zone Electrophoresis (CZE). Journal of Separation Science. 2002; 25(13):825-31. https://doi. org/10.1002/1615-9314(20020901)25:13%3C825::AIDJSSC825% 3E3.0.CO;2-I DOI: https://doi.org/10.1002/1615-9314(20020901)25:13<825::AID-JSSC825>3.0.CO;2-I

Kazmi MH, Khan S, Hameed S. Antimicrobial constituents from Pluchea wallichiana DC. Int J Bioand Biotechnol (Pakistan). 2012; 9(3):213-8

Mali PY, Goyal S. HPTLC densitometric quantification of kaempferol from leaves of euphorbia neriifolia. Indian J Pharm Educ Res. 2020; 54:s586-s92. https://doi. org/10.5530/ijper.54.3s.158 DOI: https://doi.org/10.5530/ijper.54.3s.158

Neupane P, Lamichhane J. Phytochemical profiling using HRLCMS and evaluation of antioxidant and antibacterial activities of Nepalese medicinal plants. Vegetos. 2020; 33(4):628-40. https://doi.org/10.1007/s42535-020-00143-4 DOI: https://doi.org/10.1007/s42535-020-00143-4

Lucci P, Saurina J, Núñez O. Trends in LC-MS and LC-HRMS analysis and characterization of polyphenols in food. TrAC Trends in Analytical Chemistry. 2017; 88:1-24. https://doi.org/10.1016/j.trac.2016.12.006 DOI: https://doi.org/10.1016/j.trac.2016.12.006

Shah MB. High Resolution-Mass Spectrometry (HR-MS) analysis of Bryonia laciniosa . Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]. 2022; 12(4):617-24.

Panchal H, Amin A, Shah M. Development of validated high-performance thin-layer chromatography method for simultaneous determination of quercetin and kaempferol in Thespesia populnea. Pharmacognosy Research. 2017; 9(3):277. https://doi.org/10.4103/0974-8490.210326 PMid:28827970 PMCid:PMC5541485. DOI: https://doi.org/10.4103/0974-8490.210326

Bhandare SB, Laddha KS. Simultaneous quantification of kaempferol and quercetin in medicinal plants using HPTLC. International Journal of Pharmaceutical Sciences and Research. 2016; 7(6):2379.

Laila O, Murtaza I, Abdin M, Ahmad S, Ganai NA, Jehangir M. Development and validation of HPTLC method for simultaneous estimation of diosgenin and quercetin in fenugreek seeds (Trigonella foenum-graceum). International Scholarly Research Notices. 2014;2014. https://doi. org/10.1155/2014/583047 DOI: https://doi.org/10.1155/2014/583047

Guideline ICH validation of analytical procedures Q2 (R1). International Conference of Harmonization Geneva, Switzerland; 2022.