Comparative Physicochemical Evaluation and Acute Toxicity Study of Crude and Processed (Detoxified) Samples of Saqmunia (Convolvulus scammonia L.) in Albino Wistar Rats
DOI:
https://doi.org/10.18311/jnr/2023/33454Keywords:
Acute Toxicity, Convolvulus scammonia, Detoxification, Physicochemical Standardization, SaqmuniaAbstract
Background and Objectives: Tadbīr va Islāh-i-Adwiya (detoxification/rectification of drugs) is a distinctive concept of Unani medicine in which drugs that are toxic by nature are detoxified before being administered to the human body. In Unani medicine, the resin called Saqmunia which is a derivative of the rhizome of Convolvulus scammonia, is used for therapeutic purposes. So far, limited research studies have been carried out to appraise the age-old concept of detoxification of Unani drugs. Therefore, the present study was designed to explore the physicochemical characteristics and acute toxicity of crude and detoxified samples of Saqmunia in albino Wistar rats. Methods: Saqmunia was detoxified through the tashwiya (roasting) method using apple and cow dung cakes. The physicochemical standardization includes moisture content, different ash values, extractive values; pH, fluorescence analysis, and TLC were performed. The acute toxicity study of both samples of Saqmunia was carried out at 300 and 2000 mg/kg dose levels in female albino Wistar rats. The data were analyzed using Oneway ANOVA followed by Dunnett’s comparison test. Results: The findings of the physicochemical standardization of crude and detoxified Saqmunia were found to be within an accepted range. The acute toxicity study has shown that the non-detoxified form of the test drug produced maximum toxicities, even causing death in rats. The LD50 of crude and detoxified Saqmunia was found to be 1000 mg and > 2000 mg/kg, respectively, in rats. Conclusions: The present study has suggested that Saqmunia used in this study was of good quality, and the physicochemical constants found in this study may be taken as a reference for future studies. Moreover, the acute toxicity study validates the Unani concept of detoxification of drugs, as is evident from the minimal toxic effects found in the detoxified sample of Saqmunia as compared to the crude sample in rats.
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Copyright (c) 2023 Huzaifa Ansari, Athar Parvez Ansari, Pankaj Goswami, Ifra Qayoom, Abdul Hasib, Bazilah Majeed Reshi, Seema Akbar (Author)
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This work is licensed under a Creative Commons Attribution 4.0 International License.
Accepted 2023-08-21
Published 2023-10-31
References
Rahman S, Jahan N, Makbul SA, Ahmad M, Gani MA. Scientific appraisal of Unani concept of IslaheAdvia (rectification/purification of drugs) and its importance. Journal of Ethnopharmacology. 2020; 258:1-8. https://doi.org/10.1016/j.jep.2020.112880 DOI: https://doi.org/10.1016/j.jep.2020.112880
Anonymous. National Health Portal. Gateway of authentic health information [Online]. 2015. NHP CC DC. Available at: https://www.nhp.gov.in/ayush_ ms
Kabiruddin M. Bayaz-i-Kabir, Vol. III. New Delhi: Idara Kitab al-Shifa; 2010. p. 97.
Ansari H, Ansari AP, Qayoom I, Reshi, BM, Hasib A, Ahmed NZ, et al. Saqmunia (Convolvulus scammonia L.), an important drug used in Unani system of medicine: A review. Journal of Drug Delivery and Therapeutics. 2022; 12(5):231-8. https://doi.org/10.22270/jddt.v12i5.5681 DOI: https://doi.org/10.22270/jddt.v12i5.5681
Al-Snafi AE. The chemical constituents and pharmacological effects of Convolvulus arvensis and Convolvulus scammonia- A review. IOSR Journal of Pharmacy. 2016; 6(6):64-75.
Parasuraman S, Thing GS, Dhanaraj SA. Polyherbal formulation: Concept of Ayurveda. Pharmacognosy Reviews. 2014; 8(16):73-80. https://doi.org/10.4103/0973-7847.134229 DOI: https://doi.org/10.4103/0973-7847.134229
Ansari AP, Sana SH, Ansari H. The concept of AbdāliAdwiya (drug substitution/ therapeutic interchange) in Unani medicine: A critical appraisal. Journal of Advanced Research in Pharmaceutical Sciences and Pharmacology Interventions. 2020; 3(1):1-11.
Sumbul S, Ahmad MA, Asif M, Akhtar M, Saud I. Physicochemical and phytochemical standardization of berries of Myrtus communis Linn. Journal of Pharmacy and Bioallied Sciences. 2012; 4(4):322–6.
https://doi.org/10.4103/0975-7406.103266 DOI: https://doi.org/10.4103/0975-7406.103266
Afolabi SO, Akindele AJ, Awodele O, Anunobi CC, Adeyemi OO. A 90-day chronic toxicity study of Nigerian herbal preparation DAS-77 in rats. BMC complementary and alternative medicine. 2012; 12(1):1-8. https://doi.org/10.1186/1472-6882-12-79 DOI: https://doi.org/10.1186/1472-6882-12-79
Khan NG. Khazain al-Adwiya, Vol. IV (Urdu translation by Central Council for Research in Unani Medicine). New Delhi: Dept. of AYUSH, Ministry of H and FW, Government of India; 2010. pp. 370-4.
Khan A. Muheet-i-Azam, Vol. III (Urdu translation by Central Council for Research in Unani Medicine).
New Delhi: Department of AYUSH, Ministry of H and FW, Government of India; 2014. pp. 105-10.
Sina I. Al-Qanoon fi al-Tib Vol. II (Urdu translation by Kanturi GH). New Delhi: Ejaz Publishing House; 2010. pp. 408-9.
Kabiruddin M. Ilm al-Adwiya al-Nafeesi. New Delhi: Ejaz Publishing House; 2007. pp. 159-61.
Betar I. Al-Jam‘ al-Mufredat al-Adwiyava al-Aghziya, Vol. III (Urdu translation by Central Council for Research in Unani Medicine). New Delhi: Department AYUSH, Ministry of H and FW, Government of India; 1999. pp. 51-4.
Haleem MA. Mufredat-i-Azizi (Urdu translation by Central Council for Research in Unani Medicine).
New Delhi: Department of AYUSH, Ministry of H and FW, Government of India; 2009. p. 116.
Evans WC, Evans D. Trease and Evans pharmacognosy, 16th Ed. New Delhi: Reed Elsevier India Private Ltd; 2009. p. 37, 122-5.
Ansari AP, Sana SH, Dar MY, Goswami P, Ahmed NZ. Validation of Unani concept of Abadāl-i-Adwiya (drug substitution) by physicochemical standardization and hepatoprotective activity of Aristolochia rotunda Linn. and its substitute Curcuma Zedoaria Rosc. in albino Wistar rats. Journal of Integrative and Complementary Medicine. 2021; 19(4):913-20. https://doi.org/10.1515/jcim-2020-0378 DOI: https://doi.org/10.1515/jcim-2020-0378
Kokoski CJ, Kokoski RJ, Slama FJ. Fluorescence of powdered vegetable drugs under ultraviolet radiation. Journal of the American Pharmaceutical Association. 1958; 47(10): 715-17. https://doi.org/10.1002/ jps.3030380612 DOI: https://doi.org/10.1002/jps.3030471010
Anonymous. Quality Control methods of Herbal Materials. Malta: World Health Organization (WHO); 1998. pp. 2, 9, 23-27, 29, 31, 35, 49.
Khandalwal KR, Sethi VK. Practical Pharmacognosy techniques and experiments. Pune: Nirali Prakashan; 1999. pp. 146-88.
Anonymous. OECD Guideline for Testing of Chemicals 423 [Online]. 2001. Available at: www.oecd.org
Ansari AP, Wadud A, Jahan N, Nagaraj RB, Irshad S, Iqbal F, et al. Evaluation of chronic toxicity of Kushta Sammul Far (calx of Arsenic trioxide). Journal of Xenobiotics. 2013; 3(1): 14-23. https://doi.org/10.4081/xeno.2013.e3 DOI: https://doi.org/10.4081/xeno.2013.e3
Ansari, MY. Pharmacognostical studies on Gumma (Leucas cephalotes Spreng.) [Master’s thesis].
Bangalore, KA: Rajiv Gandhi University of Health Sciences; 2011.
Anonymous. Evaluation of crude drugs. [Online]. Available at: http://www.jiwaji.edu/pdf/ecourse/ pharmaceutical/Evaluation%20of%20crude%20 drugs.pdf
Patwekar SL, Suryawanshi AB, Gaikwad MS, Pedewad SR, Potulwar AP. Standardization of herbal drugs: An overview. The Pharma Innovation Journal. 2015; 4(9):100-4.
Akula P, Lakshami PK. Effect of pH on weakly acidic and basic model drugs and determination of their ex vivo transdermal permeation routes. Brazilian Journal of Pharmaceutical Sciences. 2018; 54(2):1-8.
https://doi.org/10.1590/s2175-97902018000200070 DOI: https://doi.org/10.1590/s2175-97902018000200070
Ono M. Resin glycosides from Convolvulaceae plants. Journal of Natural Medicines. 2017; 71(4):591-604. https://doi.org/10.1007/s11418-017-1114-5 DOI: https://doi.org/10.1007/s11418-017-1114-5
Anwar N, Khan MS, Kabir H, Ahmad S. Effect of detoxification (tadbeer) in content of toxic metabolites of Strychnos nux-vomica: A Unani approach for its use in human. Journal of Pharmacy and Bioallied Sciences. 2015; 7(4):314–16. https://doi.org/10.4103/0975-7406.168034 DOI: https://doi.org/10.4103/0975-7406.168034
Akbar S, Khan SA, Masood A, Iqbal M. Use of Strychnosnux-vomica (Azraqi) seeds in Unani system of medicine: Role of detoxification. African Journal of Traditional, Complementary and Alternative Medicines. 2010; 7(4):286-90. https://doi.org/10.4314/ajtcam.v7i4.56689 DOI: https://doi.org/10.4314/ajtcam.v7i4.56689
Sulaiman CT, Deepak M, Praveen TK, Lijini KR, Anandan EM, Salman M, et al. Purification of Bhallathaka (Semecarpus anacardium L. f.) enhanced anti-cancer activity. Regulatory Toxicology and Pharmacology. 2021; pp. 122. https://doi.org/10.1016/j.yrtph.2021.104898 DOI: https://doi.org/10.1016/j.yrtph.2021.104898