Potential of Zingiber officinale Extract against Chlorpyrifos Toxicity in Caprine Testicular Cells

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Authors

  • Priyanka Saraf
     Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra – 136119, Haryana ,IN
  • Harish Panchal
     Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra – 136119, Haryana ,IN
  • Kameshwar Sharma
     Department of Biochemistry, Sri Venkateshvara College, University of Delhi, Delhi – 110021 ,IN
  • Sanjeev Sharma
     Department of Library and Information Science, Kurukshetra University, Kurukshetra – 136119, Haryana ,IN
  • Jitender Kumar Bhardwaj
     Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra – 136119, Haryana ,IN

DOI:

https://doi.org/10.18311/ti/2022/v29i4/29728

Keywords:

Apoptosis, Chlorpyrifos, Testicular Cells, Zingiber officinale.
Toxicology

Abstract

Chlorpyrifos, an organophosphate insecticide, is currently under studies due to its reproductive toxicity in non-targeted organisms. The present study on its efficacy as reproductive toxicant, revealed its effect on testicular cells of caprines. CPF was found to decrease cell viability in testicular cells, induced histo-architectural alterations like loss of tubular interactions and empty luminal spaces in testis; initiated DNA damage associated apoptosis as revealed by MTT, Histology and EB/AO fluorescence assay respectively, at both the doses (10 and 50 μM). CPF mediated toxicity induced oxidative stress in testicular cells as revealed by dose- and time-dependent increase in lipid peroxidation and decline in ferric reducing antioxidant power. However, supplementation of Zingiber officinale (ginger) extract reduced the CPF mediated decrease in cell viability, histological defects, incidence of apoptosis, and generated oxidative stress parameters; thus, proving its potential as a therapeutic agent against CPF, preventing testicular cells toxicity mediated reproductive disorders.

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Published

2023-02-07

How to Cite

Saraf, P., Panchal, H., Sharma, K., Sharma, S., & Bhardwaj, J. K. (2023). Potential of <i>Zingiber officinale</i> Extract against Chlorpyrifos Toxicity in Caprine Testicular Cells. Toxicology International, 29(4), 465–473. https://doi.org/10.18311/ti/2022/v29i4/29728

Issue

Volume 29, Issue 4, December 2022

Section

Articles
Crossref
Scopus
Google Scholar
Europe PMC
Received 2022-03-05
Accepted 2022-05-06
Published 2023-02-07

 

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