Validating the Antimicrobial Potentiality of Peptides from Pods of Acacia nilotica Willd.ex Delile: A Spotlight on Bacterial Fauna
Authors
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Department of Bioscience and Biotechnology, Banasthali Vidyapith, Tonk - 304022, Rajasthan ,IN
Richa Shukla Shukla -
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Tonk - 304022, Rajasthan ,INVeena Sharma
DOI:
https://doi.org/10.18311/ti/2023/v30i1/31892Abstract
Contagious infections cause over 17 million human deaths in a year among which diseases caused by bacteria are the most prominent ones. Additionally, in the last few years, the haphazard usage of antibiotics has provoked the condition of multifarious defiance in the bacterial strains against these chemical drugs thus contributing to an upsurge towards severe economic and communal welfare-related uncertainties. Thus, to annihilate such situations there is a compelling demand to explore novel substitutes to overcome these concerns. Hence, such problematic situations bring up the limelight towards plant-derived proteins/peptides possessing antimicrobial activity which prove to be excellent alternatives against bacterial pathogens causing serious maladies in humans. Therefore, in this context the current study investigated the antibacterial potential of the Ethanol, Methanol and Chloroform peptides/protein isolate obtained from pods of Acacia nilotica by Disc-Diffusion Assay and MIC (Minimum Inhibitory Concentration) against E. coli and B. subtilis bacterial strains. The antibacterial activity of all three solvent peptide isolates was found to be exhibiting antimicrobial activity at a concentration of 100μg/ml against B. subtilis with Disc-Diffusion as well as MIC with Chloroform isolate displaying the highest activity against gram-positive Bacillus species. Therefore, such plant-derived antibacterial proteins/peptides in the future can be used as a therapeutic drug to combat various severe bacterial maladies thus providing a better alternative than antibiotics both in terms of safety against unwanted secondary effects as well as will also help in bringing down the graphs of Antimicrobial Resistance (AMR) posing a serious threat to the human health. As a result, the study concludes that peptide isolates obtained from Acacia nilotica pods are effective against gram-positive Bacillus species, making them an important antibacterial compound.
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Accepted 2023-01-02
Published 2023-03-20
References
Datta S, Roy A. Antimicrobial peptides as potential therapeutic agents: A review. Int J of Peptide Research and Therapeutics. 2021; 27:555-577. https://doi. org/10.1007/s10989-020-10110-x
Vouga M, Greub G. Emerging bacterial pathogens: Past and Beyond. Clinical Microbiology and Infection. 2015; 22:12-21. https://doi.org/10.1016/j.cmi.2015.10.010 PMid:26493844 PMCid:PMC7128729
Vogelmann R, Amieva MR. The role of bacterial pathogens in cancer. Current Opinion in Microbiology. 2007; 10:76-81. https://doi.org/10.1016/j.mib.2006.12.004 PMid:17208515
Farsimadan M, Motamedifar M. Bacterial infection of the male reproductive system causing infertility. Journal of Reproductive Immunology. 2020; 142. https://doi. org/10.1016/j.jri.2020.103183 PMid:32853846
Prakash D, Saxena RS. Distribution and antimicrobial susceptibility pattern of bacterial pathogens causing urinary tract infection in urban community of Meerut city, India. ISRN Microbiol. 2013; 29. https:// doi.org/10.1155/2013/749629 PMid:24288649 PMCid:PMC3830820
Raghunath D. Emerging antibiotic resistance in bacteria with special reference to India. J Biosci. 2008; 33:593- 603. https://doi.org/10.1007/s12038-008-0077-9 PMid:19208984
Sang Y, Blecha F. Antimicrobial peptides and bacteriocins: alternatives to traditional antibiotics. Anim Health Res Rev. 2008; 9:227-35. https://doi.org/10.1017/ S1466252308001497 PMid:18983725
Moretta A, Scieuzo C, Petrone AM, Salvia R, Manniello MD, Franco A, Lucchetti D, Vassallo A, Vogel H, Sgambato A, Falabella P. Antimicrobial peptides: A new hope in biomedical and pharmaceutical fields. Front Cell Infect Microbiol. 2021; 11. https://doi. org/10.3389/fcimb.2021.668632 PMid:34195099 PMCid:PMC8238046
Rana D. A review of ethnomedicine, phytochemical and pharmacological properties of Acacia nilotica (Babool/Kikkar). Int J of Bio Pharmacy and Allied Sciences. 2018; 7:856-863. https://doi.org/10.31032/ IJBPAS/2018/7.5.4443
Abdalla MS, Babiker IA , Al-Abrahim JS, Mohammed AE, Elobeid MM, Elkhalifa KF. Fodder potential and chemical composition of Acacia nilotica fruits for livestock in the dry lands of Sudan. Int J of Plant, Animal and Environmental Sciences. 2014; 4:366-369.
Bauer AW, Kirby WMM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966; 45:493-496. https://doi. org/10.1093/ajcp/45.4_ts.493 PMid:5325707
Pandian CJ, Palanivel R, Dhanasekaran S. Screening antimicrobial activity of nickel nanoparticles synthesized using Ocimum sanctum leaf extract. J of Nanoparticles. 2016; 2016:1-13. https://doi.org/10.1155/2016/4694367
Salas CE, Badillo-Corona JA, Ramírez-Sotelo G, Oliver- Salvador C. Biologically active and antimicrobial peptides from plants. Biomed Res Int. 2015; 2015. https://doi.org/10.1155/2015/102129 PMid:25815307 PMCid:PMC4359881
Benko-Iseppon AM, Galdino SL, Calsa T Jr, Kido EA, Tossi A, Belarmino LC, Crovella S. Overview on plant antimicrobial peptides. Curr Protein Pept Sci. 2010; 11:181-8. https://doi.org/10.2174/138920310791112075 PMid:20088772
de Souza Cândido E, e Silva Cardoso MH, Sousa DA, Viana JC, de Oliveira-Júnior NG, Miranda V, Franco OL. The use of versatile plant antimicrobial peptides in agribusiness and human health. Peptides. 2014; 55:65- 78. https://doi.org/10.1016/j.peptides.2014.02.003 PMid:24548568
Goyal RK, Mattoo AK. Multitasking antimicrobial peptides in plant development and host defense against biotic/abiotic stress. Plant Sci. 2014; 228:135-49. https:// doi.org/10.1016/j.plantsci.2014.05.012 PMid:25438794
Gruenheid S, Le Moual H. Resistance to antimicrobial peptides in Gram-negative bacteria. FEMS Microbiol Lett. 2012; 330:81-9. https://doi.org/10.1111/j.1574- 6968.2012.02528.x PMid:22339775
