Isolation, Identification and Free Radical Scavenging Potential of Acetogenin Rich Fractions from Annona muricata Leaves

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  • Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore-641 043, Tamil Nadu ,IN
  • Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore-641 043, Tamil Nadu ,IN
  • Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore-641 043, Tamil Nadu ,IN



Isolation, Identification And Free Radical Scavenging Potential Of Acetogenin Rich Fractions From Annona muricata Leaves


Herbal extracts are indicative of the most important areas of traditional medicine in the world. In order to establish their potential as a source of phytomedicine, the study of medicinal plants is important to facilitate the proper use of herbal medicine. Annona muricata L. commonly known as Soursop belongs to the family Annonaceae. The active constituents present in the plant have preventive effects for various diseases. "Acetogenins” are powerful phytochemicals present in the Annona muricata. These compounds collectively have shown antitumor, parasiticidal, pesticidal and antimicrobial activities. The objective of the present study is to isolate, identify acetogenin rich fraction from Annona muricata leaves and to evaluate the capacity of free radical scavenging. This evidence stated that acetogenin rich fraction could be isolated from Annona muricata leaves and this important phytochemical compound was easily identified by using kedde's reagent. All the four fractions (F1C, F2C, F3C and F4C) tested for radical scavenging activity possess considerable scavenging activity, among the extracts tested F4C fraction exhibited highest scavenging activity.


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How to Cite

Ilango, S., Jayachandran, P., & Nirmaladevi, R. (2021). Isolation, Identification and Free Radical Scavenging Potential of Acetogenin Rich Fractions from <i>Annona muricata</i> Leaves. The Indian Journal of Nutrition and Dietetics, 58(S1), 81–91.



Original Articles



Sultana, S., Asif, H.M., Nazar, H.M. I., Akhtar, N., Rehman, J.U. and Rehman, R. U. Medicinal plants combating against cancer- a green anticancer approach. Asian Pac. J. Cancer P., 2014, 15, 4385-4394. doi: 10.7314/APJCP.2014.15.11.4385. DOI:

Moghadamtousi, S.Z., Kamarudin, M.N.A., Chan, C.K., Goh, B.H. and Kadir, H.A. Phytochemistry and biology of Loranthus parasiticus Merr, a commonly used herbal medicine, The Am. J. Chinese Med., 2014, 42, 23-35. DOI:

Moghadamtousi, S.Z., Goh, B.H., Chan, C.K., Shabab, T. and Kadir, H.A. Biological activities and phytochemicals of Swietenia macrophylla king, Molecul., 2013, 18, 10465-10483. https:// DOI:

Cijo George, V., Naveen Kumar, D.R., Rajkumar, V., Suresh, P.K. and Ashok Kumar, R. Quantitative assessment of the relative antineoplastic potential o f the n-butanolic leaf extract of Annona Muricata Linn. in normal and immortalized human cell lines, As. Paci. J. Cancer Preven., 2012, 13, 699-704. DOI:

Zorofchian Moghadamtousi, S., Karimian, H., Rouhollahi, E., Paydar, M., Fadaeinasab, M. and Abdul Kadir, H. Annona muricata leaves induce G1 cell cycle arrest and apoptosis through mitochondria-mediated pathway in human HCT116 and HT-29 colon cancer cells, J. Ethnopharmacol., 2014, 156, 277-289,. DOI:

Jiménez, V.M., Gruschwitz, M., Schweiggert, R.M., Carle, R. and Esquivel, P. Identification of phenolic compounds in soursop (Annona muricata) pulp by high-performance liquid chromatography with diode array and electrospray ionization mass spectrometric detection. Fd. Res. Int., 2014, 65, 42-46. DOI:

Andrade, J.M.M. and Fasolo, D. Polyphenol antioxidants from naturalsources and contribution to health promotion, Academic Press, SanDiego, USA, 2014.

Coria-Téllez, A.V., Montalvo-Gónzalez, E., Yahia, E.M., Obledo-Vzquez, E.N. Annona muricata: A comprehensive review on its traditional medicinal uses, phytochemicals, pharmacological activities, mechanisms of action and toxicity. Arab. J. Chem., 2016, 11, 662-691. DOI:

Potts, L.F., Luzzio, F.A., Smith, S.C., Hetman, M., Champy, P. and Litvan, I. Annonacin in Asiminatriloba fruit: implication for neurotoxicity. Neurotoxicol., 2012, 33, 53-58. DOI:

Vení¢ncio, V.P., Ferreira, E.B., Brigago, M.R.P.L., Paula, F.B.D.A., Barbisan, L.F. and Azevedo, L. Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesis. Ciíªn Agrotec., 2013, 37, 343-349. DOI:

Hamizah, S., Roslida, A.H., Fezah, O., Tan, K.L., Tor, Y.S. and Ton, C.I. Chemopreventive potential of Annona muricata L. leaves on chemically-induced skin papillomagenesis in mice. Asian Pac J. Cancer Prev., 2012, 13, 2533-2539. DOI:

Paulinus, O., Kinsley, A. and Ikechi, E. Protective effect of ethanolic leaf extract of Annona muricata Linn on some early events in cycas-induced colorectal carcinogenesis in rats. J. Pharm. Sci. Innov., 2013, 2, 14-21. DOI:

Lima, L.A.S., Pimenta, L.P. and Boaventura, M.A.D. Acetogenins from Annona cornifolia and their antioxidant capacity. Fd. Chem., 2010, 122, 1129-1138. DOI:

Valko, M., Leibfritz, D., Moncol, J., Cronin, M.T., Mazur, M. and Telser, J. Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol., 2006, 7, 45-78.

Ahmed, M., Khan, M.I., Khan, M.R., Muhammad, N., Khan, A.U. and Khan, R.A. Role of medicinal plants in oxidative stress and cancer. Open Acce. Sci. Rep., 2013, 2, 641.

Lobo, V., Patil, A., Phatak, A. and Chandra, N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn. Rev., 2010, 4, 118-126. DOI:

Umarani, N. and Ilango, K. Exploration of in vitro antioxidant and antimicrobial studies of Momordicacymbalariafenzyl leaves extracts. Int. J. Pharm. Pharm. Sci., 2015, 7, 235-241.

Luna, J.D.S., Carvalho, J.M.D., Lima, M.R.F.D, Bieber, L.W., Bento, E.D.S., Franck, X. and Santana, A.E.G. Acetogenins in Annona muricata L. (annonaceae) leaves are potent molluscicides. Natural Product Res., 2006, 20, 253-257. DOI:

Brand-Williams, W., Cuvelier, M.E. and Berset, C.L.W.T. Use of a free radical method to evaluate antioxidant activity, LWT-Fd. Sci. Technol., 1995, 28, 25-30. S0023-6438(95)80008-5 DOI:

Shirwaikar, A., Rajendran, K. and Kumar, C.D. In vitro antioxidant studies of Annona squamosa Linn. leaves, Ind. J. Exp. Biol., 2004, 42, 803-807. DOI:

Ruch, R.J., Cheng, S.J. and Klaunig, J.E. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogen., 1989, 10, 1003-1008. DOI:

Klein, S.M., Cohen, G. and Cederbaum, A.I. Production of formaldehyde during metabolism of dimethyl sulfoxide by hydroxyl radical-generating systems. Biochem., 1981, 20, 6006-6012. DOI:

Shirwaikar, A. and Somashekar, A.P. Anti-inflammatory activity and free radical scavenging studies of Aristolochia bracteolata Lam. Ind. J. Pharmacy. Sci., 2003, 65, 67.

Yıldırım, A., Oktay, M. and Bilaloglu, V. The antioxidant activities of the leaves of Cydonia vulgaris. Tr. J. Med. Sci., 2001, 31, 23-27.

Olorunjuwon, O. J., Michael A. Gbadegesin, Oyeronke A. Odunola, In vitro free radical scavenging and antioxidant properties of ethanol extract of Terminalia glaucescens, Pharmacognosy Res, 2015, 7, 49-56. DOI:

Avani, P., Amit, P. and Patel, N. M. Determination of polyphenols and free radical scavenging activity of Tephrosia purpurea linn leaves (Leguminosae), Pharmacognosy Res., 2010, 2, 152-158 DOI:

Correa-Gordillo, J., Ortiz, J., Sa'nchez-Mejı'a, M., Pacho'n, H. Actividad antioxidantenguana' bana (Annona muricata L.) unarevisio'nbibliogra'fica. Bol. Latinoam. Caribe Plant. Med. Aromat, 2012, 11, 111-126.

Paz, M., Gúllon, P., Barroso, M.F., Carvalho, A.P., Domingues, V.F., Gomes, A.M., Becker, H., Longhinotti E. and Deleure Matos, C. Brazilian fruit pulps as functional foods and additives: Evaluation of bioactive compounds. Fd. Chem., 2015, 172, 462-468. DOI:

Pérez-González, A., Rebollar-Zepeda, A.M., León-Carmona, J.R. and Galano, A. Reactivity indexes and OH bond dissociation energies of a largeseries of polyphenols: Implications for their free radical scavenging activity. J. Mex. Chem. Soc., 2012, 56, 241-249. DOI:

Taira, J., Tsuchida, E., Katoh, M.C., Uehara, M. and Ogi, T. Antioxidant capacity of betacyanins as radical scavengers for peroxyl radical and nitric oxide. Fd. Chem., 2015 166, 531-536 DOI: