Bioefficacy and mass culturing of Heterorhabditis bacteriophora (Nematoda:Heterorhabditidae) on cabbage butterfly, Pieris brassicae (Lepidoptera:Pieridae) in temperate region of Kashmir, Indi
Keywords:Bioefficacy, entomopathogenic nematode, Heterorhabditis bacteriophora, Pieris brassicae
AbstractA laboratory investigation was carried out to assess the bioefficacy and mass culturing of Kashmir isolate of entomopathogenic nematode (EPN), Heterorhabditis bacteriophora on cabbage butterfly, Pieris brassicae in Srinagar. The pathogenicity of H. bacteriophora against 3rd, 4th and 5th larval instars of P. brassicae showed cent per cent mortality of the pest in all the three larval instars at the dosages of 20 IJs/larva and above. The LC50 values of H. bacteriophora against 3rd, 4th and 5th larval instars of P. brassicae ranged from 46.61 to 9.32, 76.33 to 9.82 and 91.36 to 10.08 IJs/larva at 24 to 96 h and that the LT50 values from 65.15 to 23.12, 84.54 to 29.20 and 105.36 to 33.52 h at 10 to 60 IJs/larva, respectively. The average production of nematode infective juveniles per larvae was found to be 7.02 x 103, 25.74 x 103 and 58.45 x 103 and that from per gram of host body weight was 91.49 x 103, 151.30 x 103 and 233.78 x 103 in 3rd, 4th and 5th larval instars of P. brassicae, respectively. The total time period between the larval mortality and the initiation of emergence of the infective juveniles from the cadavers ranged from 7-8, 9-10 and 10-11 days and the peak period of emergence of infective juveniles ranged from 10-13, 14-19, 16-21 days after mortality from the cadavers of 3rd, 4th and 5th larval instars of P. brassicae, respectively. The total duration of nematode emergence recorded during the period between larval mortality and cessation of emergence of infective juveniles from the cadavers ranged 17-19, 23-25 and 26-29 days after mortality in case of 3rd, 4th and 5th larval instars of P. brassicae, respectively.
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