In-vivo production of a Kashmir isolate of EPN, Heterorhabditis bacteriophora (SKUASTK-EPN-Hr 01) on test insect hosts from Srinagar (J&K)
Keywords:Heterorhabditis bacteriophora, Bombyx mori, Corcyra cephalonica, Galleria mellonella, In-vivo production, virulence
Under laboratory conditions, the virulence of the Kashmir isolate of EPN, Heterorhabditis bacteriophora (SKUASTK-EPN-Hr 01) to Galleria mellonella, Corcyra cephalonica and Bombyx mori showed that after 48-72 h, 100% mortality was recorded in all the test insects. Considering 48 h time and 20 IJs/larva as standard against G. mellonella, C. cephalonica and B. mori, the LC50 and LT50 values for the EPN were 10.17, 28.72 and 23.81 IJs/larva, and 38.64, 53.04 and 49.20 h after exposure, respectively. Again its virulence to the larvae of three different sizes of these insects, viz., small (0.12g, 0.04g, 0.17g), medium (0.18g, 0.05g, 0.59g) and large (0.22g, 0.07g, 2.58g) sized larvae showed that all the three sizes of G. mellonella were more susceptible than that of B. mori and C. cephalonica. Considering 20 IJs/larva as standard against small, medium and large sized larvae, the LT50 value for the EPN was 25.00, 26.45 and 27.21 h; 47.73, 48.92 and 53.16 h, and 46.04, 47.48 and 48.92 h for G. mellonella, C. cephalonica and B. mori, respectively. The production of nematode infective juveniles per larvae was directly proportional to the size and/or body weight of the insect species tested. The average production of nematode infective juveniles per gram of host body weight ranged from 1557.79 to 1933.55 x 103, 217.38 to 335.43 x 103 and 71.03 to 128.00 í— 103 in G. mellonella, C. cephalonica and B. mori, respectively. The total time period between the larval mortality and the initiation of emergence, and between larval mortality and the cessation of emergence of nematode infective juveniles from the cadavers ranged from 7-10, 6-8 and 9-12 days, and 19-28, 16-24 and 23-41 days in G. mellonella, C. cephalonica and B. mori, respectively.
Ali SS, Ahmad R, Hussain MA, Pervez R. 2005. Pest Management in Pulses through Entomopathogenic Nematodes. Army Printing Press, Lucknow, India, 58 pp.
Flanders KL, Miller JM, Shields EJ. 1996. In vivo production of Heterorhabditis bacteriophora `Oswego' (Rhabditida: Heterorhabditidae), a potential biological control agent for soil-inhabiting insects in temperate regions. J Econ Entom 89(2): 373-380.
Forschler BT, Gardner WA. 1991. Parasitism of Phyllophaga hirtula (Coleoptera: Scarabaeidae) by Heterorhabditis heliothidis and Steinernema carpocapsae. J Inv Path 58(4): 396-407.
Georgis R. 1992. Present and future prospects for entomopathogenic nematode products. Bio Sci Tech 2(1): 83-99.
Kim JB, Park JD, Kim CS. 1995. The distribution and pathogenicity of entomogenous nematodes in forest soil in Korea. FRI J For Sci (Seoul) 51(1): 74-79.
Lisansky SG, Coombs J. 1994. Developments in the market for biopesticides. Brighton Crop Protection Conference - Pests and Diseases, pp 1049-1054.
Singh SP. 1994. (Ed.) Project Directorate of Biological Control, Bangalore, pp. 221.
Sivakumar CV, Jayaraj S, Subramanian S. 1988. Observations on an Indian population of the entomopathogenic nematode Heterorhabditis bacteriophora Poinar, 1976. J Biol Control 2(2): 112-113.
Subramanian S. 2003. In vivo production of entomopathogenic nematodes. Ins Environ 9(1): 33.
White GF. 1927. A method for obtaining infective nematode larvae from cultures. Science 66: 302-303.
Zaki FA, Mantoo MA, Gul S. 2000. In vivo culturing of entomopathogenic nematodes Heterorhabditis bacteriophora and Steinernema carpocapsae on silkworm (Bombyx mori) and their effect on some lepidopterous insects. Indian J Nematol 30(1): 1-4.
How to Cite