Growth Parameters of some Isolates of Entomofungal Pathogens and Production of Dust-Free Spores on Rice Medium

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Authors

  • Entomology section, Sugarcane Breeding Institute, Coimbatore-641007 ,IN
  • Project Directorate of Biological Control (ICAR), Post Bag No. 2491, H. A. Farm Post, Bellary Road Hebbal, Bangalore, 560 024, Karnataka ,IN
  • Project Directorate of Biological Control (ICAR), Post Bag No. 2491, H. A. Farm Post, Bellary Road Hebbal, Bangalore, 560 024, Karnataka ,IN
  • Project Directorate of Biological Control (ICAR), Post Bag No. 2491, H. A. Farm Post, Bellary Road Hebbal, Bangalore, 560 024, Karnataka ,IN

DOI:

https://doi.org/10.18311/jbc/2005/3542

Keywords:

Beauveria bassiana, Biomass, Metarhizium anisopliae, Radial Growth, Spore Production, Verticillium lecanii.

Abstract

Four isolates in each of Beauveria bassiana (Bals.) Vuill, Metarhizium anisopliae (Metschinikoff) Sorokin and Verticillium lecanii (Zimmerman) Viegas, pathogenic to key pests of crops like, coffee berry borer, coconut rhinoceros beetle, cashew stem borer, citrus and coffee green scale etc. were assessed for the colony growth on Potato Dextrose Agar (PDA), biomass and spore production in Potato Dextrose Broth (PDB) and dry spore production in rice grown through di-phasic production system. The isolates Bb5a (B. bassiana), Ma4 (M. anisopliae) and V15 (V. lecanii) were found fast growing with the colony diameter of 6.3, 8.1 and 5.7cm, respectively on PDA after 15 days of incubation. Biomass production in PDB was comparatively higher in shake cultures than in stationary cultures for all isolates except Bb4, BbSa, and V13a. Among the B. bassiana isolates, biomass production in stationary cultures was highest in Bb4 isolate (0.78 g/100ml) and in shake culture in Bb3 isolate (1.02g/100ml). Ma2 in stationary eulture (0.8g/100ml) and Ma3 isolate in shake cultures produced maximum biomass (l.09g/100ml). V1 2a isolate among V. lecanii isolates produced maximum biomass in stationary culture and shake culture (0.77 and 1.03g/100ml, respectively). M. anisopliae isolates formed mycelial pellets of various sizes under shake culture condition in PDB, In two-stage system of mass production, the maximum spore production on rice was observed in BbSa, Ma4 and V15 isolates (96.0, 49.8 and 17.5 x 108 spores g−1) and maximum spores per gram of rice were harvested in Bb5a, Ma2 and V12a isolates (28.00, 45.50 and 15.60 mg g−1). Maximum viable spores per gram of spore dust were recorded in Bb5a, Ma4 and V15 isolates (4.7 x 1010, 2.5 x 1010 and 1.7 x 109g−1). Taking into consideration of quantity of spore dust production and viable spores in the spore dust, Bb5a, Ma2 and V12a were identified as potential isolates for large-scale production of dry conidial powder.

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Published

2005-12-14

How to Cite

Nirmala, R., Ramanujam, B., Rabindra, R. J., & Rao, N. S. (2005). Growth Parameters of some Isolates of Entomofungal Pathogens and Production of Dust-Free Spores on Rice Medium. Journal of Biological Control, 19(2), 121–128. https://doi.org/10.18311/jbc/2005/3542

 

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