Post Harvest Fruit Bioassay of Phylloplane, Pomoplane and Endophytic Microbes against Chilli Anthracnose Pathogen, Colletotrichum capsici (Syd.) E. J. Butler&bisby


  • National Bureau of Agriculturally Important Insects, Bangalore, Karnataka, 560024, India


Two hundred and fifty eight phylloplane/endophytic bacterial isolated from chilli leaves/fruits and one hundred pomoplane yeast isolates from vegetable/fruits were screened against Colletotrichum capsici by fruit bioassay (post harvest) method. Among the pomoplane bacterial isolates tested, Bacillus tequilensis (PMB-185) gave highest reduction (67.84%) of lesion development, where as among the phylloplane bacterial isolates, PHB-25 exhibited highest (48.65%) suppression of lesion caused by C. capsici. Among the endophytes tested, B. megaterium (ENB-86) produced the highest suppression of lesion (59.66%) and rhizospheric bacterium Pseudomonas putida (PBA-5) showed 50.68% suppression. Six bacteria exhibiting significant suppression (50.29 to 67.84%) were identified by 16s rDNA analysis and all of them belonged to Bacillus spp. including B. tequilensis (PMB-185), B. pumilus (PMB- 183), two B. subtilis (PMB-123 and ENB-24) and two B. megaterium (PMB-53 and ENB-86). Among the yeast isolates tested, the maximum reduction (72.16%) of lesion development was observed with the yeast isolate, Hanseniaspora uvarum (Y-73) which was the highest among all the antagonists tested. The results indicated that spraying of H. uvarum (Y-73) or B. tequilensis (PMB-185) on freshly harvested chilli fruits reduced post harvest fruit damage by C. capsici in chilli.


Chilli Anthracnose, Colletotrichum capsici, Fruit Bioassay, Pichia guilliermondii and Bacillus Species.

Subject Discipline

Agriculture Sciences

Full Text:


  • There are currently no refbacks.