A Rapid <I>in vivo</I> Bioassay Method for Testing and Selection of Fungal Antagonists of Plant Pathogens

R. K. Srivastava; R. D. Prasad; R. Rangeshwaran; A. R. Wasnikar; S. P. Singh; N. S. Rao

doi:10.18311/jbc/2002/4130

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Authors

R. K. Srivastava
Crop Research Station, Narendra Deva University of Agriculture and Technology, Bahraich 271 801, U. P. ,IN
R. D. Prasad
Project Directorate of Biological Control (ICAR), P. B. No. 2491, H. A. Farm Post, Hebbal, Bangalore 560 024, Karnataka ,IN
R. Rangeshwaran
Project Directorate of Biological Control (ICAR), P. B. No. 2491, H. A. Farm Post, Hebbal, Bangalore 560 024, Karnataka ,IN
A. R. Wasnikar
Department of Plant Pathology, Jawaharlal Nehru Krishi Vishwa Vidhyalaya, Jabalpur 482 004, M. P. ,IN
S. P. Singh
Project Directorate of Biological Control (ICAR), P. B. No. 2491, H. A. Farm Post, Hebbal, Bangalore 560 024, Karnataka ,IN
N. S. Rao
Project Directorate of Biological Control (ICAR), P. B. No. 2491, H. A. Farm Post, Hebbal, Bangalore 560 024, Karnataka ,IN

DOI:

https://doi.org/10.18311/jbc/2002/4130

Keywords:

Fungal Antagonists, in vivo Bioassay, Plant Pathogens.

Abstract

Eight Trichoderma isolates were tested for their bioefficacy against seed, root and seedling rot incited by Rhizoctonia solani by adopting an in vivo test method (blotter test). Vigor index ranging from 169.5 to 2239.4 and disease incidence ranging between 10 to 86 percent were recorded in various treatments. All bioagents were graded based on disease grading key proposed for their rating. The clear differentiation of efficacy of various Trichoderma species against R. solani obtained with the in vivo bioefficacy test method adopted (blotter test) in the present study shows suitability of this method for routine screening of fungal biocontrol agents against seed and soil borne plant pathogens.